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Cordycepin as the main active ingredient of Cordyceps natural medicine , anti-tumor, anti-viral , anti-fungal, immune regulation, and a variety of biological activity , clinical studies found that loss of efficacy due to deamination , and the impact of further research . Deaminase inhibitor , likely to cause human immunodeficiency , increase the amount of the increased costs. Select a new drug carrier to solve the deamination Cordycepin important direction of current research . This article filter liposomes as drug carriers for a preliminary study . Cordyceps militaris ethanol ultrasonic method from extraction to the cordycepin crude , refined sample was purified by column chromatography using ultraviolet and infrared spectra as well as high performance liquid chromatography prepared Cordycepin qualitative . Extract lecithin, lecithin extracted factors , and filter out the the lecithin best extraction conditions . To soybean oil as raw materials , acetone, ethanol as solvent and high purity lecithin . Ethanol as solvent, the cholesterol was purified by recrystallization method . The resulting lecithin , cholesterol, refined products were compared with the standard confirmed are high-purity samples . To the homemade Cordycepin , lecithin and cholesterol as raw material , through the reverse-phase evaporation method Cordycepin liposomes , and the products were TEM characterization , entrapment efficiency , stability testing results obtained liposome pale yellow spherical particle diameter of 170 to 350 nm , the encapsulation efficiency was 45% , and good stability . The cordycepin liposomes in vitro experimental results show that Cordyceps liposome can effectively inhibit the U937 proliferation of tumor cells , the protective effect of the liposomes to avoid cordycepin adenosine deaminase degradation , increased Cordyceps the efficacy of hormone , reducing the role of the effective dose . The caterpillar fungus liposome U937 tumor cell proliferation inhibition rate when the the cordycepin content to 0.08 mg , with 2.36 times the concentration Cordycepin .
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