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Protein Quality Evaluation and Innovation of Soybean Germplasm in Zhejiang Province

Author: JiangYing
Tutor: ZhuDanHua
School: Zhejiang Normal University
Course: Botany
Keywords: Soybean Germplasm Storage protein 11S/7S ratio Lipoxygenase Lectin
CLC: S565.1
Type: Master's thesis
Year: 2010
Downloads: 53
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Abstract

Soybean storage protein is soybean important part soybean nutritional quality and characteristics of one of important factors. Zhejiang Province and its surrounding areas like eating soybeans and soybean processing products, the region soy food consumption. Focus on the quality of life in the modern society, the nutritional quality of soy protein to the concern, therefore, improve the nutritional quality of soy protein soybean breeding expert solved one of the important topics of the experiment soybean sub-centers in Hangzhou, Zhejiang saved part summer soybean germplasm resources for research material, for soybean storage protein-7S globulin and 11S globulin and its subunits, soybean lipoxygenase, soybean lectin, etc., SDS-denaturing polyacrylamide gel electrophoresis, color and spectrophotometry methods tested soybean germplasm research and analysis, achieved the following key findings: 1 found through analysis of the tested soybean germplasm protein electrophoresis 7S globulin α 'α subunit content Zhejiang Province within the soybean germplasm material or low 4 parts: ZDD06245, ZDD06063, ZDD13928 and ZDD06066, and found 30KD to 17KD unknown small molecule peptide. Zhejiang Province, 343 soybean germplasm material 7S globulin changes in the relative content of the range between 0.152-0.371; 11S globulin changes in the relative content in 0.428-0.728:11 S/7S ratio range between 1.270-4.008, with an average 2.291; the 7S globulin relative content is less than 20%, while the relative content of 11S globulin 5 were higher than 60% of the germplasm, accounting for 1.46% of the total; province 262 soybean germplasm material 7S globulin relative content , 11S globulin the Zhejiang Province soy relative changes in the content and 11S/7S ratio range of 0.181-0.389,0.403-0.696,1.425-3.369,11 S/7S ratio average of 2.025, the highest value and the average than those tested germplasm highest value, low average. Tested 343 soybean germplasm materials in Zhejiang Province the in 11S/7S ratio higher than 3.0 the 26 germplasm material 11S/7S ratio higher than 4.0, 1, and outside the province's 262 soybean germplasm material 11S / 7S ratio higher than 3.0 and 14. All tested soybean germplasm materials 7S globulin ratio relative content and 11S/7S of germplasm coefficient of variation greater than 0.10, indicating that soybean seed protein content and composition of the existence of large genetic variation. The study also found that, Zhejiang province in northern Zhejiang Province and the northern provinces Huanghuai the germplasm materials 11S/7S (excluding the northeast and northwest of spring soybean) ratio than those in the same classification regions, Description the soybean germplasm protein 11S/7S ratio distribution has ecological culture. Soy protein and 7S globulin and 11S globulin subunits correlation analysis showed that: the total content of the soy protein 7S globulin is relatively content, 11S globulin relative content and 11S/7S of the ratio is not relevant, and soybean 7S globulin relative content The relative content of 11S globulin was highly significant negative correlation. Missing in soybean lipoxygenase wild soybean germplasm material found of of 1 part the soybean lipoxygenase Loxl and Lox2 missing: Variety Selection ZYD04511; the five soybean lipoxygenase Lox2 missing the germplasm materials: ZDD06271 ZDD06044, ZDD13834 ZDD06245 and VP35; the soybean fat the oxidase Lox2 and Lox3 missing wild soybean germplasm material: ZYD04452 3, this experiment 150Gy 60Coγ ray irradiation '3618 'germplasm, 1 part soy lectin subunit mutants ; compared to normal '3618 'germplasm mutant soybean agglutination factors to be genetically stable mutant trait; the M6 ??generation of this mutant plant from flowering bloom 16 days until 46 days after soybean lectin expression in different developmental stages character study, initially identified Soybean Agglutinin in about 34 days after flowering synthesized starting to mature content gradually increased; with soybean lectin mutant early from synthetic exhibit mutant trait may stage began at the molecular level expression mutated.

Protein Quality Evaluation and Innovation of Soybean Germplasm in Zhejiang Province

Abstract

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