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Melaleuca tower , scientific name Huperzia serrata ( Trev , Huperzia serrata ( Thunb.ex Murray , ) , ) , is one of the traditional Chinese herbal Huperzia genus (Huperzia) plants , huperzine A (Huperzine A) is its main biologically active substances . Pharmacology studies have shown that Huperzine A is an important low toxicity, efficient , highly selective and reversible inhibitor of acetylcholine , a variety of effects with the treatment of Alzheimer's , myasthenia gravis , schizophrenia disease . Melaleuca tower shoot tips as explants , the establishment of the the Melaleuca tower tissue culture system , induced to obtain a green spherical body and profusion of seedlings , and to study the impact of hormones on the growth of clusters of seedlings synthetic huperzine . The main findings are as follows : ( 1 ) Melaleuca Fatah Establishment of culture system . The study found that the the , the December acquisition Melaleuca tower shoot tip to the tissue culture explants higher success rate , 2% sucrose 1/4MS mineral elements 1/2MS organic elements modified MS medium is the most suitable for the growth of explants . With carried cultured on a modified MS medium , the four types of explants morphology observed occurred, namely: green spherical body , two fork branches , multi - fork branch , and goes fork branch . ( 2) hormone profusion seedling growth and synthesis of huperzine . Material profusion seedlings , effects of auxin ( indole acetic acid IAA , indole butyric IBA ) and cytokinin ( 6 - benzyl aminopurine BA , kinetin KT) the profusion seedlings growth and synthetic huperzine impact . The results showed that the synthesis of IAA and IBA on the profusion seedling growth and accumulation of huperzine with the promotion of the role of higher concentrations of BA ( gt; 2 μM) ; , and KT ( gt ; 5μM) profusion seedling growth and huperzine to a certain extent ; After 60 days of culture , the effect of 10 μM IBA the profusion seedlings growth , growth rate of 9.4 mm / month , and new leaves form 4 / biomass increased 5.1 times ; 2μM IAA huperzine A synthesis is more favorable , after 40 days of culture , content to reach 612.3μg / g DW ; 10 μM IBA can significantly promote the profusion rooting and root growth ; 2 μM BA favor the profusion of the regeneration of seedlings . Results not only for the in-depth study of huperzine A biosynthetic pathway raw technical information , but will also provide a new way to solve Huperzine A shortage of resources .
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