Dissertation > Excellent graduate degree dissertation topics show

Studies on the Processing Procedures of Huperzia Serrata and HupA In-situ Forming Gel

Author: ZhangZuo
Tutor: WangJinHui
School: Shenyang Pharmaceutical University
Course: Traditional Chinese Medicine and science
Keywords: Melaleuca tower Vinegar Huperzine A PLGA-PEG-PLGA copolymers Gel Vivo correlation
CLC: R283
Type: Master's thesis
Year: 2008
Downloads: 40
Quote: 0
Read: Download Dissertation

Abstract


Chinese medicine Melaleuca tower as Huperzia Huperziaceae is Huperzia Serrata plant Huperzia serrata (Thunb.) Trev. Grass synonyms: lice grass, dwarf cedar lice po drugs, life-saving king, raw pull rope. Melaleuca tower only contained in the plant name, map test \Eighties, China's pharmaceutical scientists and technicians from the Melaleuca tower isolated strong bioactive alkaloids Huperzine A (Huperzine A HupA). Huperzine A as a clinical treatment of Alzheimer's disease drugs, is an efficient and highly selective inhibitor of acetylcholinesterase. Scattered due to the Distribution of Melaleuca tower resources, resource update cycle is longer for the exploitation of natural resources, the reasonable use of the existing resources, vinegar, wine system processing methods to improve the extract in huperzine content. The research results show that the wine made, vinegar made Melaleuca Tati extracts of huperzine A content were 0.016%, 0.020%, the huperzine content than uncooked food extracts 0.0095% doubling therefore Melaleuca tower of the rational use of the new means. The use of modern chromatographic means from the total alkali vinegar Melaleuca tower isolated two compounds, and were identified by chemical and spectroscopic methods Huperzine A (HuperzineA, Hup A) and huperzine B (Huperzine B Hup B). Through the establishment of the determination the Melaleuca tower preparation technique and the extraction process in huperzine A method for the determination, using single factor and orthogonal design experiments, and ultimately establish a the Huperzia serrata vinegar manufacturing process and optimum conditions of the extraction process. Huperzine A as a treatment for Alzheimer's drugs, tablets, injections, capsules are listed, Huperzine A short biological half-life, clinical need multiple daily dosing, This article was prepared Huperzine A temperature-sensitive PLGA-PEG-PLGA block copolymer subcutaneous injection of sustained-release gel in order to achieve the purpose of to extend the duration of action of the drug, to reduce the number of medication to reduce adverse reactions, improve patient compliance. Huperzine in vitro and in vivo analysis methods, and huperzine nature. The results showed that in vitro and in vivo analysis method is accurate, reliable, simple, and able to meet the analytical requirements; seen by solubility experiments, the solubility of Huperzine A in pH7.4 PBS solution about 1mg/ml; 25 ° C when Huperzia alkali A positive octanol - water system in the oil-water partition coefficient of 10.02. The film dispersion method was successfully prepared the huperzine injectable sustained release gel. The effects of different prescription and technological factors on the in vitro drug release behavior. Determined on the basis of this best prescription and craft, the best prescription process repeatability visits, the results show that the process reproducibility and stability. Finally, huperzine injectable sustained-release gel rat in vivo pharmacokinetic studies in vivo analysis method was determined by HPLC. The results show that: huperzine injectable sustained release gel formulations T max 3h; the C max (112.96 ± 10.64) ng · ml -1 ; AUC 0 → T (2331.30 ± 364.90) ng · h · ml -1 ; the the AUC 0 → ∞ ( 3016.22 ± 400.57) ng · h · ml -1 ; t 1/2 (39.420 ± 2.41) h. In vivo sustained release time for 3 to 4 days, a good correlation between in vitro and in vivo. In summary, on the best vinegar process of traditional Chinese medicine Melaleuca tower and the optimal conditions of the extraction process, so that the content of the active ingredient in the extract huperzine were improved: by film dispersion method Huperzine A injectable sustained-release gel, results of in vitro and in vivo studies indicate that injectable sustained release gel sustained release four days, can be reached to extend the time of drug action, the purpose of reducing the number of drugs.

Related Dissertations

  1. Preparation and Photocatalytic Properties for Water Decomposition of Titanate Photocatalyst,O643.36
  2. Preparation of ITO Quai-1D Nanostructures by Sol-Gel Method Combined with Porous Anodic Aluminum Oxide Template,TB383.1
  3. Research on Feed Safety of Transgenic Rice in Broiler,S831.5
  4. Cryopreservation and Damage Research of Sperm of Cobia (Rachycentron Canadum) and Crimson Snapper (Lutjanus Erythopterus),S961.2
  5. Preparation and Performance of V2O5-WO3/MOx/Cordierite Catalyst Modified with Cu、Mn、Ce for DeNOx,X701
  6. Study on Processing Functionalities and Application of Bovine Plasma Proteins,TS251.1
  7. Effect of Wheat Straw Wood Vineger on Grain Yield and Qualities of Rice,S511
  8. Establishment of Transgenic Soybean, Maize and Wheat Informationg Platform and Effect of Transgenic Soybean on Soil Microorganisms,S565.1
  9. Effects of Mannose-oligosaccharide on Intestinal Morphology and Bacterial Community in Gastrointestinal Tract of Broiler Chickens,S831.5
  10. Iron, lanthanum -doped nano TiO \u003csub\u003e 2 \u003c/ sub\u003e Preparation and photocatalytic properties,O614.411
  11. Multiple intermediate silicate silicate polymerization system theory,O613.72
  12. Preparation and Properties of Filtering Ceramics for High Temperature Exhaust Gas,TQ174.6
  13. Preparation and Characterization of Doped BiFeO3 Nanocrystals,TB383.1
  14. Investigation into ZnO Thin Film Transistors Prepared by Sol-gel Method,TN321.5
  15. Early Cytogenetic Damage of Plywood Workers Occupationally Exposed to Formaldehyde,R131
  16. Nano- phenyl silicone resin preparation and application,TB383.1
  17. α crystal protein and retinal ganglion cell membrane associated proteins of exploration and study,R774.6
  18. Boron nitride fiber reinforced ceramic matrix composites through the waves and Properties,TB332
  19. Establishment of Double Antibody-Sandwich ELISA for Detecting E.ovis,S855.99
  20. Neonatal intensive care unit , multi-drug resistant Acinetobacter baumannii resistance mechanisms and homology,R446.5
  21. Nano- TiO_2 composite photocatalytic oxidation of elemental mercury removal experimental study,X51

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Chinese medicine processing,preparation
© 2012 www.DissertationTopic.Net  Mobile