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Molting is an important physiological behavior of the insect growth and development, the insect's body wall due to the hardening of the outer skin, the formation of the exoskeleton, hardened exoskeleton ductility, so the insect body is the growth, development must periodically molt process. Molt, the insects do not eat less dynamic, less harmful to the crop, not molt, the larvae can not becoming an adult, the formation of the food consumption is reduced, there is no vitality or can not reproduce the abnormal individual. Therefore, the insect molting process has been a pest control research hotspot. Molting that will not only help to clarify the the insect molt physiological mechanism, also helps to find a new insecticide targets. Early molting research focuses on the ecdysone regulation of molting regulate transcription factor expression and molting fluid Purification involves molting Cloning and Functional Analysis rarely reported. Zhang our laboratory for the study of the Oriental migratory locust, isolated and cloned a putative encoded product tryptase serine protease gene, named LM-TSP, the use of RNAi technology study found that injection of LM-TSP-specific dsRNA oriental migratory locust larvae from the fourth instar to fifth instar larvae molt from fifth instar larvae to adult blocked, and ultimately death. LM-TSP gene silencing cause molting fluid protease activity and capsid protein degradation activity decreased. These results indicate that the LM-TSP gene oriental migratory locust molting related, suggesting that the gene can be used as Locusticide target the gene for the study, and further analysis to verify its functionality. Analysis of the gene in the fifth instar larvae to adult expression of different developmental stages, and the expression of the fifth instar larvae of different tissues and organs; the embryo incubation process involved in molting, we also analyzed the gene in different incubation time of oriental migratory locust expression in the egg; induced by Pichia pastoris means of expression for functional studies of the LM-TSP; also clone the gene isoforms. The subject to study the insect molting physiological development process, looking for a new insecticide targets are of great significance. The main results are as follows: 1, the analysis of the oriental migratory locust fifth instar larvae to adult LM-TSP gene expression at different developmental stages. The gene 72h, 24h, 16h fifth instar larvae expressed mainly in the emergence, after the decline in the amount of expression. 2, oriental migratory locust fifth instar larvae of different tissues and organs Lm-TSP gene expression. The gene main fifth instar larvae torso, head and hind expression almost no expression in blood cells, midgut and fat body. 3, embryo hatching the molting process involves analysis of the Lm-TSP gene expression of different incubation period of locust eggs. LM-TSP gene expression in Locusta migratoria eggs, but the expression amount is lower expression levels increase with extended incubation time. 4, LM-TSP in Pichia pastoris expression 45kD bands detected a treaty, which laid the foundation for the next step to continue to study. The 5 cloned five isoforms of Lm-TSP gene sequence. Were named Lm-TSPA, Lm-TSPB, Lm-TSPC, Lm-TSPD, Lm-TSPE.
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