|
Objective: In this study, rat limb replantation model to detect edaravone on serum superoxide dismutase (SOD), malondialdehyde (MDA) and skeletal muscle mitochondrial Na-K-ATP enzyme, Ca2-ATP activity and light microscopy, electron microscope, skeletal muscle structure changes, Xie Ming edaravone on rats after replantation of severed limbs protection. Methods: 40 a clean healthy female SD rats were randomly divided into four groups (n = 10): S sham operation group, M group limb replantation model group, E1 edaravone low-dose group, E2 group The edaravone high dose group. M group: rats were anesthetized after the success of fixed along the femoral vessels in the the right hind conventional skin disinfection direction of travel longitudinal skin incision to expose and free the femoral artery and vein and femoral nerve, blunt separation of the layers of tissue, cut at the level of the middle of the right femur All thigh muscle to the femur, leaving only the blood vessels. Right hind limb exsanguination, vascular clips occlusion of the right femoral artery and vein, at room temperature for 3h, relax vascular folder, the formation of reperfusion, the suture muscles, nerves and skin. Surgical field with saline soaked sterile gauze. By tail vein before reperfusion give sodium heparin 1000U/kg. E1, E2 group in addition to the same as the operation of the M group, before reperfusion by intravenous injection of edaravone 0.5mg/kg and 1mg/kg. S and M groups given saline. S group to free the femoral artery and vein, exclusion 3h after skin closure. Each group at reperfusion 5h and 24h, respectively, the external jugular vein blood each lml centrifugation after standing, the determination of serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. And reperfusion 24h taken right hind gastrocnemius, to extract mitochondrial determination of skeletal muscle mitochondrial Na-K-ATPase enzyme, Ca2-ATPase activity changes. In another right hind tibial anterior muscle structural changes in skeletal muscle in the light and electron microscope. Results: 40 rats in the result analysis. The 1 gross morphology observed: S group right hind limb muscles, skin red and warm. M group right hind limb muscles, skin color is a little dark, slightly lower temperature. The E1 group right hind appearance change than the M group reduced. The E2 group right hind appearance is a significant improvement compared with the M group. 2 skeletal muscle tissue sections observed: normal S group of muscle fibers, interstitial edema, no inflammatory cell infiltration. M group muscle fiber structural disorder, disintegration, vascular dilatation and congestion, interstitial edema, inflammatory cell infiltration of more. E1 group of muscle fibers edema, interstitial edema, slightly inflammatory cell infiltration. The E2 group interstitial slightly edema, muscle scattered inflammatory cell infiltration. 3 TEM observation: S group myofibrils arranged in neat rows, Z line is clear. Mitochondria dense, membrane integrity, no swelling. M group myofibrillar part of the fracture, Z line is blurry, mitochondrial swelling significantly, crest disorders, vacuoles. E, the original group of muscle fibers arranged in neat, Z line is a little fuzzy, contain more lipid droplets, mitochondrial part vacuolization. E2 group myofibrils arranged in neat, Z line is clear, containing a small amount of lipid droplets, mitochondrial part vacuolization. 4 SOD activity and MDA content: SOD activity: group S than M, E1, E2 group (P <0.05) lower SOD activity. Than with group M, E1, E2 group SOD activity were increased (P lt; 0.05). The E1 group than in the E2 group SOD activity increased (P <0.05). 24h after reperfusion, M, E1, E2 group SOD activity compared to 5h. The content of MDA: S group than M, E1, E2 MDA levels significantly increased (P <0.05). M group than E1, E2 group MDA content were lower (P <11.05). The E1 group than in the E2 group MDA content decreased (P <0.05). 24h after reperfusion, M, E1, E2 content of MDA 5h. The S group 24h after reperfusion of the two indicators and reperfusion 5h difference was not statistically significant (P> 0.05). 5 skeletal muscle mitochondrial Na-K-ATPase enzyme, Ca2-ATPase activity: group S than M, E1, E2 group Na-K-ATPase enzyme, Ca2-ATP enzyme were lower (P <0.05). M group than E1, E2 group Na-K-ATPase enzyme, Ca2-ATP enzyme were increased (P <0.05). E1 than E2 group Na-K-ATPase enzyme Ca2-ATPase enzyme increased (P <0.05). Conclusion: 1 edaravone reduce rat limb ischemia-reperfusion injury, has a protective effect on the replantation after amputation of limbs, and the high-dose group was better than the low-dose group. 2 and its mechanism may be increased SOD activity and decreased MDA content, increase in skeletal muscle mitochondria Na K-ATPase enzyme, Ca2-ATPase activity.
|