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Effect of Intraoperative Interorganizational Injection Chemotherapy Targeted on Ki-67, Apoptosis in Metastatic Lymph Nodes of Advanced Gastric Carcinoma
Author: XuXueFeng
Tutor: JiangHuaiWu
School: Luzhou Medical College
Course: Surgery
Keywords: Advanced gastric cancer Targeted chemotherapy Ki-67 gene Apoptosis
CLC: R735.2
Type: Master's thesis
Year: 2010
Downloads: 56
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Abstract
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Objective: To understand the injection targeted chemotherapy transfer lymph Ki-67 expression and apoptosis (apoptosis, AI) , explore the treatment of advanced gastric cancer surgery to strengthen the effectiveness of advanced gastric cancer surgery organization . Methods : Radical preoperative cut stomach No. 3 No.6 or No.7 lymph nodes in a rapid frozen section confirmed as metastatic cancer patients included in the study , to meet the requirements of 28 patients with advanced gastric cancer were randomly divided into two groups direct surgical control group ( treatment group and the control group ) , the treatment group in the tumor tissue and surrounding injection of methylene blue and mitomycin mixture ( of methylene blue 2ml mitomycin 8mg0.9 % NS20m1 ) after surgery, . Surgery take metastatic lymph nodes with 4% paraformaldehyde fixed , paraffin-embedded , HE staining , each group select 20 of metastatic lymph nodes (treatment group were selected staining ) prepared for the study . Specimens (including the radical former specimens ) the effectiveness of observation and ki-67 expression, apoptosis detection . Results: Before treatment, the transfer of lymph node tumor apoptosis and necrosis of the validity of the difference was not statistically significance ( P gt ; 0.05 ); before treatment of metastatic lymph nodes ki-67 expression , apoptosis index was no statistical difference ( P gt ; 0.05) ; after treatment, the metastatic lymph node tumor cell apoptosis , necrosis of the validity of ( rate ) than control group , the difference was statistically significant (P lt; 0.05); after treatment, the transfer of lymph node Ki-67 expression positive rate is lower than before treatment , the apoptotic index is higher than before treatment , before and after differences with statistical significance ( P lt ; 0.05 ); after treatment, the metastatic lymph nodes Ki-67 expression is lower than that of the control group , the apoptotic index higher than that in control group , the difference between the two groups was statistically significant (P lt; 0.05) Conclusion: The inter-organizational injection targeted chemotherapy can inhibit metastasis of gastric cancer lymph node tumor cell proliferation and promote apoptosis of tumor cells have a direct role in the treatment of lymph node metastasis stove ; organization targeted injection of chemotherapy treatment is an effective technique to strengthen , to prevent iatrogenic proliferation micrometastases , micro - residue and postoperative recurrence and metastasis may have a certain effect .
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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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