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Study of the Gastric Mucosa Cancer Cells Using Raman Spectroscopy

Author: DaiJianHua
Tutor: PengGuiYong
School: Third Military Medical University
Course: Internal Medicine
Keywords: Raman spectroscopy Surface Enhanced Raman Spectroscopy Gastric mucosal cells Intestinal metaplasia Canceration Genomic DNA
CLC: R735.2
Type: Master's thesis
Year: 2010
Downloads: 137
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Abstract


Gastric cancer is one of the most common malignant tumor, the global prevalence and mortality are high, our country is also a high incidence of gastric region. The incidence of gastric cancer tend to go through the normal gastric mucosa - atrophy - intestinal metaplasia - dysplasia - cancer, cancerous gastric mucosal cells can be divided according to the degree of malignancy of high, medium, low, undifferentiated. Various diseased cells generate all of its internal structure is the embodiment of change. May be better understood by studying the different stages of the cell structure characteristics, the process of the occurrence of gastric cancer. Raman spectroscopy is a very good method of research material and spatial structure, which is formed by molecules of high-frequency inelastic scattering of incident light phenomenon emission spectra. Raman spectroscopy is used for the biological sample test has many advantages, such as: the biological sample is lower - the sample morphology may be a gas, liquid or solid; small amount of sample, can be a minor or trace; less susceptible to the interference of the water, water environment can be used to detect; without damage to the sample; fast test organisms to achieve real-time diagnostics, dynamic observation of the cells, using near-infrared laser can avoid fluorescence interference. Raman spectroscopy is just to meet the requirements of the biological sample test has been widely applied to the biomedical field. Currently, Raman spectroscopy has been used for the study of tissue, cells, etc., by the measured spectral analysis structural characteristics observed dynamic changes. But for gastric mucosal cells from the normal development of the cancer process, the various characteristic Raman spectroscopy study also little. Raman spectra of normal gastric mucosa, intestinal metaplasia and cancerous tissue and genomic DNA, and different degree of differentiation of gastric cancer cell line genomic DNA discriminate analysis, the main results are as follows: 1. Using laser confocal Raman spectrometer Raman spectra characteristic detection HE staining of normal tissues of gastric mucosa, intestinal metaplasia and adenocarcinoma cell nucleus. Through a comparative analysis, the results suggest that: Raman spectroscopy to detect normal and intestinal tissue sections is quite similar, and the Raman spectra of the cancerous tissue was significantly different from the previous two regions separately, can be very good. Raman spectrum peaks in the cancerous tissue mainly in: 1) 600 00cm -1 963cm -1 performance relative strength strong peaks; nucleic acids, proteins and carotenoid content in the cancerous tissue is higher; 2) in 1090 cm , -1 at peak intensity decreased and shifted to 1088 cm -1 < near / sup>, suggesting that DNA single-and double-stranded break occurred; 3) in 1610 cm -1 at band intensity was significantly weaker than the 1526 cm -1 at The band intensity, normal tissue in the contrary, it shows that the protein in the cancerous tissue with respect to the content of the lipids of carotenoids reduce. Cell genomic DNA extraction technology will be organized with Raman spectroscopy combined approach, the normal tissues of gastric mucosa, intestinal metaplasia and cancerous tissue genomic DNA extract used for Raman detection of Raman spectrum analysis, the results suggest that the stability of the phosphate backbone: the genomic DNA of normal tissue of gastric mucosa; intestinal metaplasia genomic DNA Raman characteristic peak at 1090 cm -1 at peak intensity is less than 1050 cm -1 at a peak intensity that its phosphate backbone becomes unstable; adenocarcinoma genomic DNA Raman peaks near 1090 cm -1 doublet, relative to the 1050 cm -1 at the stronger the intensity of the spectral peaks, suggesting the DNA there may scission and again the formation of a stable phosphate backbone at 950 cm , -1 , 1010 cm -1 , 1100 1 600 cm -1 band characteristic peaks compared with normal tissue DNA change is also larger, indicating deoxyribose and base may cause changes in the structural symmetry of the DNA chain scission. We can infer that gastric cancer may be in the process of changes in the structure of DNA: gastric normal tissue stable DNA phosphate backbone pathogenic factors, the development of intestinal metaplasia with unstable phosphate backbone of DNA, the last tissue DNA phosphate backbone fracture and re-forming a stable DNA phosphate backbone induced gastric carcinogenesis. Normally cultured gastric mucosal epithelial cell line (GES -1 ), well-differentiated gastric cancer cell line (MKN-28), moderately differentiated gastric cancer cell line (SGC-7901), poorly differentiated gastric cancer cell lines (BGC-823) and undifferentiated gastric cancer cell line (HGC-27), and cell genomic DNA extraction, the to extract surface enhanced Raman spectroscopy detection. Analysis of the Raman spectrum: with normal gastric mucosa cells than the well-differentiated cell genomic DNA Raman spectra in addition to be enhanced in 1010 cm -1 at both spectral spectrum is extremely similar;, undifferentiated cell genomic DNA Raman spectra in the 750-800 cm -1 , 890 cm -1 , 960 cm -1 < / sup>, 1010cm -1 at the crescendo peaks, 1060 cm -1 , 1090 cm -1 seen at weak peaks. These peaks may be prompted to the higher the degree of malignancy of the cell genome DNA has a more vibration mode is activated, the relative strength of the stronger; less differentiated cell genomic DNA structural arrangement even more diversified chain scission; simultaneously with compared to normal cells, the DNA of the overall chargeability may also occur a change. System study of gastric cancer in the course of various Raman spectroscopy, and help us to a better understanding of its structure variation of Raman spectroscopy applied to the real-time diagnosis of clinical disease, early diagnosis, study the pathogenesis observed drug efficacy have guiding significance. This thesis, the application of Raman spectroscopy to detect normal gastric mucosa, intestinal metaplasia and cancerous tissue and genomic DNA, as well as the degree of differentiation of gastric cancer cell lines genomic DNA for gastric cancer pathogenesis of early clinical diagnosis and research new ideas.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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