|
Research purposes. Polymorphism distribution analysis Gansu region of Han and Hui population the Ghrelin gene Arg51Gln and Lue72Met sites; 2. Explore Ghrelin gene polymorphism and Gansu Han, Hui type 2 diabetes and insulin resistance, blood pressure, blood lipids correlation of risk factors for diabetes. Objects and method objects Han patient group (T2DM) 113 patients (58 males, 55 females), mean age 56.68 ± 13.84 years, the control group (NGT), 106 patients (58 males and 48 females), mean age 55.11 ± 14.98 years; the Hui Nationality cases group (T2DM) 137 patients (male 71 cases, female 66 cases), the average age of 53.80 ± 11.90 years, the control group (NGT) 112 patients (68 cases, 44 females), mean age 54.29 ± 14.59 years old. Study each other unrelated objects. Type 2 diabetes are in line with the provisions of the 1999 World Health Organization diagnostic criteria, and the control group were healthy adults of the same period outpatient physical examination were excluded from serious heart, liver and kidney diseases. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method, exon 2 outside of the study Ghrelin gene on Arg51Gln, and Leu72Met polymorphism detection; simultaneously anthropometry, blood pressure (BP), fasting plasma glucose (FPG), fasting insulin (FINS), the detection of blood lipids, creatinine (CR), uric acid (UA) and other laboratory parameters, calculate the waist-to-hip ratio (WHR), body mass index (BMI), insulin resistance index (HOMA-IR) and islet p-cell function (HOMA-β). The establishment of a database, SPSS13.0 software for statistical analysis using Excel. Results Gansu Han, Hui population Ghrelin gene Arg51Gln exists, and Leu72Met Loci; 2 of ghrelin the gene Arg51Gln and Leu72Met genotype GG, GA, AA and CC, CA, AA; allele G, A and C, A frequency difference between type 2 diabetes mellitus group and the control group, the Hui type 2 diabetic group and the control group was not statistically significant (all P gt; 0.05), ghrelin gene Arg51Gln, genotype distribution and allele gene frequency differences between the Han and Hui was statistically significant (P lt; 0.01); groups of type 2 diabetes in Han, Hui GA AA genotype of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) were higher than the GG genotype; while the control group, GA AA genotype TC than GG genotype, the difference was statistically significant (P lt; 0.05); 4 of the Han control group CC genotype TC higher than CA AA genotype; TC higher than CA AA genotype in type 2 diabetes group Hui CC genotype, were higher than the control group, the CC genotype TC, LDL-C CA AA genotype, the difference was statistically significant (all P lt; 0.05). The remaining indicators showed no significant difference (P gt; 0.05). Research conclusions. Gansu Han, Hui population Ghrelin gene Arg51Gln exists and Leu72Met bit point mutation and Arg51Gln locus polymorphisms in different ethnic distribution of frequency; Ghrelin gene Arg51Gln and Leu72Met mutations in Gansu Province Han, Hui 2 independent of diabetes; 3 of ghrelin gene Arg51Gln of, and Leu72Met mutation dyslipidemia.
|