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The gramineus the active ingredients β - asarone anti- Alzheimer's rat model of the role and mechanism of hippocampal apoptosis

Author: LiChengChong
Tutor: NiuYingCai
School: Jiamusi University
Course: Pharmacy
Keywords: Senile dementia Shichangpu β - asarone Apoptosis PT-PCR
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 288
Quote: 2
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Abstract


Objective To investigate Shichangpu the active ingredient beta-asarone anti Aβ1-42 bilateral hippocampal injection induced AD model rat hippocampal neuronal apoptosis and its molecular mechanisms. 140 method adult male SD rats were randomly divided into blank control group, sham operation group, model control group, hydrochloric donepezil control group, β-Asarum ether low dose group (12.5 mg / kg · d), β-fine octyl ether middle dose group (25 mg / kg · d) and β-asarone high dose group (50 mg / kg · d). Bilateral hippocampal injection of Aβ1-42 induced Alzheimer's rat hippocampal nerve cell apoptosis model. 7 d after administration, modeling ig 28 d. Hippocampal cell apoptosis by TUNEL assay, using immunohistochemical detection of Bax, Bcl-2 and Caspase-3 protein expression; quantitative real-time RT-PCR analysis of Bax, Bcl-2 and Caspase-3 mRNA expression; Western blot assay Bax, Bcl-2 and Caspase-3 protein expression. TUNEL assay apoptosis in rat hippocampus: The results showed that β-asarone low-dose group [(32.0 ± 5.0) apoptotic cells / mm2, the middle dose group [(24.4 ± 5.5) apoptotic cells / mm2] high dose group [(18.0 ± 5.2) apoptotic cells / mm2] with the model control group [(50.2 ± 5.1) apoptotic cells / mm2] compared a significant difference, indicating that β-asarone AD hippocampal nerve cell apoptosis significantly inhibited. Qualitative immunohistochemical detection results show that: the normal control group and the sham group hippocampus of cleaved Caspase-3, Bax expression less Bcl-2 expression more; model control group and donepezil hydrochloride Qi Haima organization of cleaved Caspase-3, Bax expression was significantly increased Bcl-2 expression was significantly reduced; β-asarone dose hippocampus of cleaved caspase-3 and Bax expression significantly reduced Bcl-2 expression was significantly increased; quantitative detection of the Western blot results show that: the hippocampus cleaved Caspase ,(165.9 ± 23.2)%] with significant differences; quantitative real-time RT-PCR experiments results show that: β-asarone each dose group reduced the expression of Bcl-2 mRNA was significantly inhibited, Bax and Caspase-3 mRNA expression increased significantly inhibited. Conclusion The experiments show that β-asarone Aβ1-42 bilateral hippocampal injection induced AD model rat hippocampus neuronal apoptosis has a protective effect and its mechanism of action may be in the nerve cells by inhibiting Bax, Caspase-3 expression and promote the expression of Bcl-2, and the dose-effect relationship.

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