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Part I: tonifying the kidney, liver ovulation disorders affect the purpose of the model rat HPOA: of model rat HPOA impact study comparing kidney and dredging liver Ovulatory similarities and differences to explore tonifying the kidney, liver treatment of ovulatory dysfunction the mechanism of the disease, in order to enrich the theory of traditional Chinese medicine reproductive, and promote the development of Chinese Gynecology. : Model: use the 9-day-old healthy female SD rats born the same batch of 204, weighs about 15g. Randomly selected 170 rats, a single injection of testosterone propionate subcutaneously in the back of the neck of 1.25mg / (0.05ml /) modeling. 22 weaning, free food, keeping the same conditions. Vaginal opening 70 days old model of continuous vaginal epithelial cell smears two periods (4-5 days) a sexual cycle, vaginal epithelial cells continued keratosis prompt androgen induced ovulation obstacles rat model of successful modeling. More than 34, a one-time injection of neutral tea oil 0.05ml subcutaneously in the back of the neck as the normal control group. Model: selection of 204 3-month-old healthy female Wistar rats, weighing 180 2 50g. 170 randomly selected rats improved Zhengjia Run, Liang Wenbo and other methods. Tripterygium 35mg/kg/d (dose 20 times the clinical adult dosage), gavage administration for 57 consecutive days. Made module continuous vaginal epithelial cells smear two cycles estrous cycle into irregular, major obstacles rat model of successful modeling cycle is incomplete and extended cycle, suggesting that triptolide induced ovulation. The remaining 34 gavage to distilled water as a control group. Both ovulation disorders successful model rats were randomly divided into the model group and the treatment group, the treatment group include: kidney to regulate menstruation party high dose group, Bushen Tiaojing side low dose group, spearheading the high-dose group, spearheading the low-dose group, every 34 each group; treatment group were orally kidney to regulate menstruation party spearheading the model group and normal group fed with distilled water, 1 day. Ig five weeks and vaginal cell smear two cycle. Fasted for eight hours after the last dose, select the same estrous cycle (proestrus, metestrus) decapitated blood, preparation of plasma, serum by radioimmunoassay plasma follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen diol (E 2 ), progesterone (P), testosterone (T), prolactin (PRL). Light microscopy fixative immediately aseptic operation to remove part of the ice tray adenohypophysis, ovaries, uterus, weighed and quickly and gently into pre-cooling and internal fixation. Results: 1 modeling results: (1) Tripterygium induced ovulation disorder model serum reproductive hormones compared with normal group, before and after E 2 reduce emotional (both P lt; 0.05), emotional ago the after reducing PRL (P lt; 0.05, P lt; 0.01), emotional before and after the LH increased (all P lt; 0.01) estrous late T increased (P lt; 0.05) increased proestrus P (P lt ; 0.01). Ovary and uterus weight index did not change compared with normal group (all P gt; 0.05). Have mild abnormalities of the pituitary, ovaries, uterus morphology. (2) androgen induced sterile the model rat serum reproductive hormone, in comparison with the normal group, and emotionally before and after FSH lower (all P lt; 0.01), proestrus LH lower (P lt; 0.05), emotional after PRL liter high (all P lt; 0.01), emotionally before and after T increased (all P lt; 0.01), to emotionally before after E 2 increased (all P lt; 0.01). Emotionally before after ovarian mass index, emotional, the uterine weight index and the normal group were lower (all P lt; 0.01) showed abnormal morphology of the pituitary, ovaries, uterus morphology. The 2 kidney rat HPOA two models: (1) Tripterygium induced the Ovulatory model group Bushen therapy, compared with the model group, Bushen Tiaojing low group enables the the model rats metaestrus E 2 increase (P lt; 0.05), returned to the normal level (P gt; 0.05). Proestrus LH was significantly lower (P lt; 0.01), returned to the normal level (P gt; 0.05). Allows damage to the pituitary, ovary, uterus back to normal. Kidney to regulate menstruation high group allows the model rat proestrus E 2 increased significantly (P lt; 0.01), returned to the normal level (P gt; 0.05). Metestrus E 2 increased significantly (P lt; 0.01), and higher than normal (P lt; 0.05). Metaestrus PRL increase (P lt; 0.05), returned to the normal level (P gt; 0.05). The proestrus P was significantly lower (P lt; 0.01), returned to the normal level (P gt; 0.05). Emotional before and after the LH lower (P lt; 0.01, P lt; 0.05), returned to normal levels (all P gt; 0.05). Metaestrus T lower (P lt; 0.05), and returned to the normal level (P gt; 0.05). Allows damaged pituitary, ovary, uterus morphology returned to normal. (2) androgen induced ovulation model group Bushen therapy, compared with the model group, Bushen Tiaojing low group the estrous modeling before and after FSH increased (P lt; 0.01, P lt; 0.05) can return to the normal levels (all P gt; 0.05). The ovarian mass index of metaestrus significantly increased (P lt; 0.01), returned to the normal level (P gt; 0.05). Metestrus uterus weight index increased significantly (P lt; 0.01), returned to the normal level (P gt; 0.05). Emotionally before after E 2 decreased (all P lt; 0.05), returned to normal levels (all P gt; 0.05). Emotional ago, after PRL significantly lower (all P lt; 0.01), and returned to the normal level (P gt; 0.05). Emotional before and after the T was significantly lower (all P lt; 0.01), restored to normal levels (all P gt; 0.05). Can improve the damaged ovaries, uterus, pituitary. Before the the Bushen Tiaojing high group can estrous modeling after FSH increase (P lt; 0.01, P lt; 0.05), returned to normal levels (all P gt; 0.05). Proestrus LH was significantly increased (P lt; 0.01), returned to the normal level (P gt; 0.05). Emotionally before, after ovarian mass index increased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Before estrus, uterine weight index increased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Worked up before the after E 2 decreased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Emotional before and after PRL decreased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Emotional before and after the T was significantly lower (all P lt; 0.01), restored to normal levels (all P gt; 0.05). Allows damaged ovaries, uterus, pituitary basically back to normal. 3 Shugan rat HPOA two models: (1) Tripterygium caused by the the Ovulatory model group and dredging liver treatment, compared with the model group spearheading the low group enables modeling estrous after PRL increased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Proestrus LH lower (P lt; 0.01), returned to the normal level (P gt; 0.05). Allows damaged pituitary, ovary, uterus morphology returned to normal. Spearheading the high group enables the model rats metaestrus E 2 increase (P lt; 0.05), returned to normal levels (P gt; 0.05). Emotional ago, after PRL increased (all P lt; 0.01), returned to normal levels (all P gt; 0.05). The lower modeling, estrous early P to normal levels (P gt; 0.05). Emotional before and after LH was significantly lower (all P lt; 0.01) returned to normal levels (all P gt; 0.05). Allows damaged pituitary, ovary, uterus morphology returned to normal. (2) after the androgen kidney treatment, compared with the model group, spearheading the low group enables modeling the rat estrous ago, after FSH increase (P lt; 0.01, P lt; 0.05), returned to normal levels (all P gt; 0.05). Increase modeling rat proestrus LH to normal (P gt; 0.05). Emotional before and after PRL decreased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Emotional before and after the T was significantly lower (all P lt; 0.01), restored to normal levels (all P gt; 0.05). Can improve the damaged ovary, uterus, pituitary morphology. Spearheading the high group allows modeling of rat proestrus FSH was significantly increased (P lt; 0.01), and higher than normal (P lt; 0.05). Metestrus FSH increased (P lt; 0.05), returned to the normal level (P gt; 0.05). The increased modeling rat proestrus LH higher than normal (P lt; 0.05). Estrous increase modeling before, after uterine weight index to normal levels (all P gt; 0.05). The metestrus E 2 reduce (P lt; 0.05), returned to normal levels (all P gt; 0.05). Emotional before and after PRL decreased significantly (all P lt; 0.01), returned to normal levels (all P gt; 0.05). Emotional before and after the T was significantly lower (all P lt; 0.01), restored to normal levels (all P gt; 0.05). Can improve the damaged ovary, uterus, pituitary morphology. Tonifying the kidney, of Shugan law affect the comparison of the two models in rats HPOA: (1) Tripterygium model group induced ovulation disorders, kidney Tiaojing high group the model rats metaestrus E can 2 significantly higher than spearheading the higher than spearheading the high group (P lt; 0.05), emotional pre PRL below spearheading the high group (P lt; 0.05), kidney Tiaojing low group estrous late E 2 group (P lt; 0.01), the metestrus LH is higher than spearheading the low group (P lt; 0.05), emotional early PRL below spearheading the low group (P lt; 0.05), metaestrus PRL below spearheading the low group ( P lt; 0.05) in improving ovarian morphology, kidney to regulate menstruation, low group, the number of follicles, mature follicle diameter, number of layers of the GC, the number and arrangement of dense degree of superior spearheading the high and low groups. (2) androgen induced ovulation model group, the group emotional kidney Tiaojing high, after ovarian mass index was significantly higher than spearheading the high group (all P lt; 0.01), metestrus uterus weight index higher than spearheading the high group (P lt; 0.05), kidney Tiaojing low group estrous the early LH lower than spearheading the low group (P lt; 0.05). In improving the pituitary, spearheading the high number of low group anterior pituitary gland cells, sinusoids than the number of kidney to regulate menstruation, the low-dose group. Improve ovarian morphology, the shape of the kidney to regulate menstruation high group to be rough in spearheading the high group, theca cell layer is thinner than spearheading the high group, the number of follicles, follicular diameter, number of luteal higher than spearheading the high group, GC layer number, the number and arrangement of density higher than spearheading the high group. In improving the uterine morphology, kidney, the low group, the thickness of the myometrium, the number of the gland, the continuity and integrity of the intimal layer of columnar cells superior to Xiaoyao high, the low group. Part II: tonifying the kidney, liver and the purpose of human ovarian granulosa cells function: ovarian granulosa cell culture, observed two ovarian granulosa cells function, to explore two of ovarian autocrine system occurrence of ovulation disorders and ovarian granulosa cell secretory function of the relationship between kidney to regulate menstruation party with spearheading the similarities and differences between human ovarian granulosa cell secretory function effects for of Bushen Tiaojing side with spearheading the treatment ovulation disorders clinical application to provide experimental evidence . Methods: 21-week-old female rats were randomly divided into 7 groups according to body weight, daily normal saline blank control group 2ml/100g; Bushen Tiaojing party group in the high-dose group and spearheading the, , the low-dose group were give of Bushen Tiaojing side and spearheading the. In each group one hour after the last administration of rat femoral artery blood. Room temperature for 2h, 3000r/min centrifugal 10min, the supernatant, 56 ° C water bath for 30 min inactivated complement, 0.22μm sterile filter sterilization, packaging, -20 ℃ and stored. Select the age of 20-30 years of age, healthy, Ovulatory infertility treatment in the outpatient department, Medical College of Hebei Medical University, four female volunteers. B ultrasound monitoring is no mature follicles, and exclude ovarian, uterine organic disease, bilateral tubal angiographic results show patency past March and not taking hormone. Respectively, oral of Bushen Tiaojing side and spearheading the. Medication continuously for three days, four days fasting medication (medication before fasting 12h), taking the first-day dose, 1 hour after the last administration venous blood 5ml allowed to stand at room temperature for 2h, 3000r/min centrifugal 10min, take on Ching, 56 ℃ water bath for 30 min inactivated complement, 0.22μm sterile filter sterilization packaging. Preparation of blank serum: age 20-30 years of age, health, menstrual regularity, B-monitoring mature follicles, and no ovaries, a uterus organic diseases of female volunteers, fasting venous blood sampling 5ml, the rest of my colleagues containing serum preparation. Stored at -20 ℃. From Hebei Province, the Second Affiliated Hospital of assisted reproductive Branch fetch follicular fluid containing granulosa cells by 2000r/min centrifuged, and the supernatant was discarded, precipitation granulosa cells were washed with DMEM medium for 0.5Percoll suspensions by density gradient 2500 r / min centrifugation 30min separation to remove red blood cells. Adding 0.25% trypsin, 37 ° C digestion of about 5-10min, added a solution containing 10% FBS and 1640 medium to terminate the digestion. Washed with PBS and centrifuged. By trypan blue staining, cell viability, 85% -90%, containing 10% fetal bovine serum and RPMI 1640 medium to adjust the concentration of the granulosa cells the single cell suspension of 2 × 105, the single cell suspension was placed in 24-well of the culture plates, each plate 1 ml and cultured in a 5% CO2, 37 ° C incubator. Adherent cells after 24h. Join of Bushen Tiaojing side and spearheading the people containing serum and rat serum containing made of high-dose (18%), medium dose (12%), low dose (6%) treated cells 48h. Plus on normal human serum as a blank control. Results: 1 the kidney Tiaojing square containing serum people luteinized granulosa cell secretory function of: (1) human serum containing: ① E 2 , P, cAMP level: blank group In comparison, the kidney to regulate menstruation, low group E 2 higher than the blank group (P lt; 0.05, P lt; 0.01, P lt; 0.01). Kidney to regulate menstruation in group P was significantly lower than the control group (P lt; 0.01). The Bushen Tiaojing, high group cAMP significantly higher than the control group (all P lt; 0.01). Pairwise comparisons, Bushen Tiaojing high group E 2 above the kidney to regulate menstruation, low group (P lt; 0.05, P lt; 0.01), the Bushen Tiaojing the group E 2 above the kidney to regulate menstruation low group (P lt; 0.01). The Bushen Tiaojing low group P than kidney Tiaojing in group (P lt; 0.05, P lt; 0.01). Kidney to regulate menstruation, the high group cAMP significantly higher than Bushen Tiaojing low-group (all P lt; 0.01). ② on the the FSHR expression of affect: Western blot: kidney to regulate menstruation, the FSHR expression of the low group are higher than the control group, kidney to regulate menstruation the FSHR expression of the group to be higher than Bushen Tiaojing low-group. RT-PCR method: Compared with the control group, the kidney to regulate menstruation, the high group FSHR expression was significantly higher than the control group (all P lt; 0.01). Pairwise comparisons kidney Tiaojing in FSHR value was significantly higher than kidney to regulate menstruation, low group (all P lt; 0.01), and kidney to regulate menstruation high group FSHR significantly higher than kidney Tiaojing low group (P lt; 0.01). (3) P450, PPARγ expression: Compared with the control group, the kidney to regulate menstruation, the high group P450 expression is higher than the blank group (P lt; 0.01, P lt; 0.05). Kidney to regulate menstruation, low group PPARγ expression lower than the control group (all P lt; 0.01). Pairwise comparisons kidney to regulate menstruation group of P450 expression was significantly higher than in the kidney to regulate menstruation, low group (all P lt; 0.01). Kidney to regulate menstruation, group PPARγ expression was significantly lower than kidney Tiaojing high group (all P lt; 0.01). (2) rat serum containing ① E 2 , P, cAMP levels of impact: Compared with the control group, the kidney to regulate menstruation high in group E 2 high in the control group (all P lt; 0.01). Kidney to regulate menstruation in group P was significantly lower than the control group (P lt; 0.01). Kidney to regulate menstruation, the high group cAMP significantly higher than the blank group (P lt; 0.05, P lt; 0.01). Two kidney to regulate menstruation, higher than in group E 2 kidney Tiaojing low group (all P lt; 0.01). P the Bushen Tiaojing group below the kidney to regulate menstruation, low group (P lt; 0.05, P lt; 0.01). Kidney to regulate menstruation high group cAMP than kidney Tiaojing low group (P lt; 0.05). ② on the the FSHR expression of affect: Western blot: kidney to regulate menstruation, low group FSHR expression were higher than the control group, the kidney to regulate menstruation in the group than kidney Tiaojing low group. RT-PCR method: Compared with the control group, the kidney to regulate menstruation, the high group FSHR expression was significantly higher than the control group (all P lt; 0.01). Pairwise comparisons kidney to regulate menstruation, the high group FSHR expression was significantly higher than kidney Tiaojing low group (all P lt; 0.01). (3) P450, PPARγ expression: Compared with the control group, the kidney to regulate menstruation, the high group P450 expression is higher than the blank group (P lt; 0.05, P lt; 0.01). Kidney to regulate menstruation group PPARγ expression was significantly lower than the control group (P lt; 0.01). Pairwise comparisons kidney to regulate menstruation the group P450 expression of kidney Tiaojing high is higher than in the group (P lt; 0.01, P lt; 0.05). Kidney to regulate menstruation group PPARγ expression was significantly lower than the kidney to regulate menstruation, the high group (P lt; 0.05, P lt; 0.01). 2 spearheading the drug-containing serum of human luteinized granulosa cell secretory function of (1) human serum containing: ① E 2 , P, cAMP levels of impact: Compared with the control group, spearheading high, medium and low group E 2 higher than the control group (all P lt; 0.01). Spearheading the high group P was significantly lower than the control group (P lt; 0.05). Spearheading the low, medium and high group cAMP significantly higher than the control group (all P lt; 0.01). Pairwise comparisons, spearheading the high group E 2 above spearheading the low group (P lt; 0.01). Spearheading the high group cAMP significantly higher than spearheading the low group (all P lt; 0.01). The ② FSHR expression: Western blot: spearheading the high, medium and low group expression were higher than the control group, spearheading the high group than in spearheading the low in the group, spearheading the group higher than spearheading the low group. RT-PCR method: Compared with the control group, spearheading the high group FSHR expression was significantly higher than in the control group (all P lt; 0.01). Pairwise comparisons, spearheading the high group FSHR higher than spearheading the low group (all P lt; 0.01). (3) P450, PPARγ expression: Compared with the control group, spearheading the high group P450 expression was significantly higher than the blank group (P lt; 0.01, P lt; 0.05). Spearheading the high, medium and low group expression of PPARγ lower than the control group (P lt; 0.01, P lt; 0.01, P lt; 0.05). Two high spearheading the group P450 was significantly higher than spearheading the low group (all P lt; 0.01). Spearheading the low group PPARγ expression below spearheading the high group (P lt; 0.01, P lt; 0.05). (2) rat serum containing: ① E 2 , P, cAMP levels of impact: Compared with the control group, spearheading, in group E 2 high in the control group (P lt; 0.01, P lt; 0.01, P lt; 0.05). Spearheading the high group P was significantly higher than the blank group (P lt; 0.05). Spearheading the high group cAMP significantly higher than the control group (all P lt; 0.01). Pairwise comparisons, spearheading the the high group E 2 higher than spearheading the lower in the group (all P lt; 0.01) the spearheading the the group E in 2 was significantly higher than Happy The Pill low group (P lt; 0.01). In spearheading the low group P was significantly lower than spearheading the high group (all P lt; 0.01). Spearheading the high group cAMP than spearheading the low group (P lt; 0.05, P lt; 0.01). ② FSHR expression. Protein blotting: spearheading the high, the low group were higher than the control group, spearheading the high group than in spearheading the low in the group, spearheading the group higher than spearheading the low group. RT-PCR method: Compared with the control group, spearheading the high group FSHR expression was significantly higher than the blank group (P lt; 0.01). Two, spearheading the high group than in spearheading the medium and low group (P lt; 0.05, P lt; 0.01). (3) P450, PPARγ expression: Compared with the control group, the high group spearheading the P450 was significantly higher than the blank group (P lt; 0.05). Spearheading the group PPARγ expression was significantly lower than the control group (P lt; 0.01). Two serum containing human luteinizing granulosa cell function affected comparison: (1) containing serum group: ① E 2 , P, cAMP level impact: kidney Tiaojing low group E 2 below spearheading the high group (P lt; 0.01). Kidney to regulate menstruation in group E 2 above spearheading the low group (P lt; 0.05). The the Bushen Tiaojing the high group E 2 higher than spearheading the high, medium and low group (all P lt; 0.01). Kidney to regulate menstruation in group P below spearheading the low group (all P lt; 0.01). Kidney to regulate menstruation cAMP is lower than the low group spearheading the middle group (all P lt; 0.01). Kidney to regulate menstruation group of cAMP than spearheading the low group (P lt; 0.05), below spearheading the high group (P lt; 0.01). Kidney to regulate menstruation cAMP is lower than the high group spearheading the high group (P lt; 0.01). ② FSHR expression. Protein blotting: kidney to regulate menstruation, FSHR expression in the low group were higher than the corresponding spearheading the high, medium, low group. Higher than the the FSHR expression of spearheading the high group and of Bushen Tiaojing low group FSHR expression. RT-PCR method: the kidney Tiaojing low group FSHR expression below spearheading the high school group (P lt; 0.05, P lt; 0.01). Kidney to regulate menstruation group FSHR expression above spearheading the high, medium, low group (all P lt; 0.01). Kidney to regulate menstruation high group FSHR expression above spearheading the low in the group (all P lt; 0.01). ③ the P450, PPARγ expression: Bushen Tiaojing low group P450 below spearheading the high group (P lt; 0.01, P lt; 0.05). Kidney to regulate menstruation group P450 above spearheading the low group (P lt; 0.01). Kidney to regulate menstruation P450 higher than the high group spearheading the low group (all P lt; 0.05). Kidney to regulate menstruation low group PPARγ below spearheading the high group (P lt; 0.05). PPARγ the Bushen Tiaojing group higher than spearheading the low group (P lt; 0.05), below spearheading the high group (P lt; 0.01). Kidney to regulate menstruation the high group PPARγ above spearheading the low group (all P lt; 0.01). (2) rat serum containing: (1) of E 2 P cAMP levels: the Bushen Tiaojing low group E 2 below spearheading the high in the group (all P lt; 0.01). Kidney to regulate menstruation in group E 2 higher than spearheading the low group (P lt; 0.05, P lt; 0.01). The the Bushen Tiaojing the high group E 2 higher than spearheading the low group (all P lt; 0.01). Kidney to regulate menstruation P is lower than the low group spearheading the high group (P lt; 0.01). P the Bushen Tiaojing group below spearheading the high group (P lt; 0.05, P lt; 0.01). Kidney to regulate menstruation high group P below spearheading the high group (P lt; 0.01). Kidney to regulate menstruation cAMP is lower than the low group spearheading the middle group (all P lt; 0.01). Kidney to regulate menstruation group cAMP below spearheading the high group (P lt; 0.01). Kidney to regulate menstruation cAMP is lower than the high group spearheading the high group (P lt; 0.01). ② FSHR expression. Western blot: kidney to regulate menstruation, low group of people luteinized granulosa cell FSHR expression corresponding spearheading the low group. Kidney to regulate menstruation in the group of people luteinized granulosa cell FSHR expression above spearheading the high, medium and low groups FSHR expression. RT-PCR method: Bushen Tiaojing low-group FSHR expression below spearheading the high group (P lt; 0.05). Expression of the kidney Tiaojing group FSHR above spearheading the low group (P lt; 0.05, P lt; 0.01). The FSHR expression of the kidney to regulate menstruation high group above spearheading the low group (all P lt; 0.01). ③ the P450, PPARγ expression: Bushen Tiaojing low group P450 below spearheading the high group (P lt; 0.01, P lt; 0.05). Kidney to regulate menstruation group P450 above spearheading the low group (P lt; 0.01). Kidney to regulate menstruation P450 higher than the high group spearheading the low, medium and high group (all P lt; 0.05). Kidney to regulate menstruation group PPARγ below spearheading the low and high groups (all P lt; 0.05). The high kidney to regulate menstruation group PPARγ lower than the spearheading the in group (P lt; 0.05). Conclusion: (1) two ovulation disorder model serum reproductive hormone disorders, pituitary, ovary, uterus morphology were abnormal; androgen induced ovulation model of ovarian, uterine weight index decreased. Androgen induced ovulation reduced to re-model of ovarian, uterine shape and weight index in the Tripterygium induced ovulation disorder model. (2) tonifying the kidney, liver law by improving the pituitary, ovarian, uterine morphology, regulating reproductive endocrine hormone levels, increased ovarian, uterine weight index, role to play to promote follicle development and maturation, ovulation disorders. Kidney method to improve ovarian, uterine morphology, increased ovarian, uterine weight index, better than dredging liver to regulate ovarian secretion of serum reproductive hormone, dredging liver in improving the pituitary, regulating pituitary secretion of serum reproductive hormone superior Shugan law. (3) kidney Law Shugan method can improve the human luteinized granulosa cell secretory function, regulate ovarian autocrine system, improve ovarian function role. The kidney 2 , of FSHR improve human luteinized granulosa cells E to reduce PPARγ level and expression than Shugan law, of Shugan law method was superior to improve human luteinized granulosa cell cAMP levels kidney law
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