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This paper successfully prepared a new type of activated carbon fiber / PLGA copolymers (ACF / PLGA) bone tissue engineering composite scaffold stent hydrophilic, cell adhesion and histocompatibility following specific research : (1) Preparation of a certain strength and having a good adsorption performance of activated carbon fibers (ACF), and the surface of the activated carbon fiber, the hydrophilic modifier. Rayon-based carbon fiber as raw material, the concentration 0.1mol / L the NaH2PO4 solution is impregnated pretreatment at 800 ° C, 850 ° C and 900. C physical - chemical activation method, a surface rich in macroporous activated carbon fiber prepared by steam activation, and found that the activation temperature of the rayon-based carbon fibers of ACF into holes and yield, density, surface-sectional morphology, specific surface area, fiber pull extensor strength and other properties have a great impact on yield, density and tensile strength of the downward trend, as the activation temperature increases, the number of fiber surface hole is gradually increased, the ratio of the surface area and the adsorption capacity of methylene blue. 900 ℃ activated from the activated carbon fiber, specific surface area, pore size distribution and the overall performance is superior to other temperature ranges are prepared samples of methylene blue adsorption capacity. 900 ° C activation resulting activated carbon fiber surfaces, chemical and plasma treatment, to make the surface hydrophilic group, there are more-COOH,-OH, which is conducive to cell adhesion. (2) to explore the impact of the of stent porosity of the support structure and biological properties of polylactic acid-glycolic acid (PLGA). Solvent casting / particle leaching prepared porosity of 55 ± 2%, 65 ± 2% and 75 ± 2% three-dimensional PLGA bone tissue engineering scaffold porosity on the bracket aperture distribution has little effect, but the stent The surface morphology, compressive strength and degradation rate. The compressive strength of the stent as the stent porosity increases and decreases, the rate of degradation of the stent increases with increasing porosity. L929 cell transplantation in three porosity stent, 1,3,5 and 7 days of cell activity, a porosity of 75 ± 2% of the stent on cell proliferation more favorable, the viscosity of the cells in this porosity bracket attached better and PLGA scaffolds displayed in mice, one month after the tissue slice organization does not have an adverse immune response occurs. Although the impact of the compressive strength of the stent, but there are conducive to the growth of the cells thus indicating higher porosity. (3) in a fixed porosity investigate doping ACF on the structure and properties of the PLGA scaffolds. PLGA and surface treatment of ACF as raw material, using the method of solvent casting / particle leaching system ACF / PLGA bone tissue engineering composite scaffolds, the added amount of ACF were 0%, 2.75% and 8.26%, respectively. Found that ACF Add amount of the surface morphology of the composite scaffolds, compressive strength, hydrophilicity and pore size distribution has an impact. With the increase in the amount of ACF add, hydrophilicity is gradually increased, the pore size distribution of the stent is more reasonable, but the compressive strength of the composite scaffold is slightly reduced. L929 cells transplanted into the bracket of three ACF content on culture, cells with ACF / PLGA composite scaffolds have good adhesion and normal proliferation. ACF / PLGA stent in ACF content was 8.26% of the mice one month after the tissue slices show that the organization has no adverse immune reaction occurs, and ingrowth into a more uniform distribution of the composite stent tissue relatively pure PLGA scaffolds, tissue To better compatibility. Overall, the PLGA composite scaffold doped ACF superior to pure PLGA scaffolds. The paper selection ACF doped PLGA preparation of composite scaffolds for bone tissue engineering, the scaffold has good hydrophilic effect of cell adhesion and tissue compatibility, and has potential applications in the field of bone tissue engineering.
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