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Separation/Preconcentration of Sulfonamides in Environment by Polypropylene Glycol-Based Aqueous Two-Phase Systems

Author: JieXueQiao
Tutor: ZuoYongSheng
School: Jiangsu University
Course: Applied Chemistry
Keywords: Aqueous two- phase extraction Polypropylene glycol Organic salts Inorganic salts Sulfa antibiotics
CLC: X832
Type: Master's thesis
Year: 2010
Downloads: 144
Quote: 2
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Abstract


Sulfa antibiotics are widely used in the prevention and treatment of human and animal bacterial infections, these antibiotics will be discharged into the aquatic environment with human and animal feces or urine. The sulfa antibiotic pollution of the water environment and their potential ecological harm has caused widespread concern at home and abroad. It is necessary to establish a fast and efficient method of separation / enrichment in environmental water samples sulfa antibiotic residues. We build a polypropylene glycol (PPG) - salt aqueous two-phase systems, phase separation ability of different salt solution of polypropylene glycol, and laid a solid foundation for further analysis and application of the aqueous two-phase system of theory and practice. The first time the the polypropylene glycol aqueous two-phase extraction technology and fluorescence spectrophotometry / high-performance liquid chromatography coupled with, and successfully used for the study of the sulfa antibiotic residues in environmental water samples. The main findings are as follows: (1) measured at T = 298.15 K, PPG400 inorganic salts (K3PO4/K2CO3/K2HPO4) H2O ternary system phase diagram. The screening has been the best equation fitting applies double line data in PPG400-inorganic salt aqueous two-phase system; the statistical geometry-based method, the calculated K3PO4, K2CO3, K2HPO4, the three salt in the solution of PPG400 effective discharge volume ( EEV), draw three inorganic salts phase separation: K3PO4 gt; K2HPO4, gt; of K2CO3. Then use Setschenow-type, Othmer-Tobias and Bancroft equations verified the the experiment resulting liquid - liquid equilibrium data for accuracy; The final calculated from three plait point system. (2) Determination of T = (298.15,308.15,318.15) K, PPG 400 organic salt (K3C6H5O7/K2C4H4O6/K2C2O4) of H2O aqueous two-phase system of liquid - liquid equilibrium data. Screened apply to PPG400-organic salt aqueous two-phase system of double line data fitting equation. The relative ability through the to calculated the three kinds of salt in PPG400 solution EEV value, derived its points: K3C6H5O7 gt; K2C4H4O6 ≈ K2C2O4. Investigated the influence of the temperature on the double line, the results show that gradually increases as the temperature rises, the two-phase region. Finally, the liquid - liquid equilibrium data into the Othmer-Tobias and Bancroft equations to verify the accuracy of the experimental data. (3) establish a double PPG400-NaH2PO4 aqueous phase extraction joint molecule fluorescence spectroscopy of trace sulfamethazine separation / enrichment and environmental water samples. Were investigated the influence of the quality of the salt, the solution pH, centrifugation time and temperature, and draw the optimum conditions of the extraction. Experimental results show that the salting-out effect and hydrophobic effects play an important role in the extraction process. Under optimal conditions, the amount of spiked 10.0 and 20.0μg · L-1 Shi actual water samples spiked recoveries base 93.2-97.7% and 1.5-3.8% relative standard deviation. (4) to establish a joint performance liquid chromatography separation / enrichment and environmental water samples sulfamethoxazole antibiotics PPG400-salt aqueous two-phase extraction. Effects of salt type, the amount of salt added, the pH value and the influence of temperature, and to further explore the mechanism of aqueous two-phase, split-phase and extraction mechanism. Under optimal conditions, the measured content of the the sulfa antibiotic in the Yangtze River water, Yudai river, fish pond water and tap water spiked amount of 5.0 and 10.0μg · L-1 pm, sample recoveries were 96.0-100.6% The relative standard deviation of 1.0-2.4%. The results show that the, polypropylene glycol aqueous two-phase system is an effective separation / enrichment of environmental water samples trace sulfa antibiotics.

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CLC: > Environmental science, safety science > Environmental Quality Assessment and Environmental Monitoring > Environmental monitoring > Water quality monitoring
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