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Genetic Diversity, Origin and Genetic Differentiation of Ten Sheep Breeds Using Mitochondrial DNA D-loop Region

Author: GuoYanBin
Tutor: WangHui
School: Shandong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Sheep Mitochondrial DNA Alternative ring Genetic Diversity Genetic distance Origin Population expansion Genetic differentiation
CLC: S826
Type: Master's thesis
Year: 2010
Downloads: 87
Quote: 1
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Abstract


In this study, the structure and variability of the complete sequence of the sheep mitochondrial DNA D-loop area, eight local sheep breeds from five provinces in China and two varieties introduced the origin and genetic diversity for indigenous sheep breeds of science Paul species and effective use of the foundation. Measured on the basis of the total of 133 individuals of 10 species mitochondrial DNA D-loop region complete sequence, phylogenetic analysis, the two lineages of A and B, while 10 sheep populations in genetic structure and population expansion analyzed. The main findings are as follows: nucleotide variations of to 1.10 sheep breeds mitochondrial DNA D-loop region of mtDNA D-loop region length of 133 individuals of 10 domesticated sheep breeds for 1106 1 182bp, by length proportion speculated that the length of the D-loop region of 1181bp. Apart from a small number of insertion / deletion length variation is mainly the result of the different number of repetitions of the tandem repeat. In addition, the three length for the the 1176bp sequence in consecutive four bases (CCAC) Absence 143-146 at there. The average content of 133 individual A, T, G, C, respectively, 33.0%, 29.7%, 14.4% and 22.9%, respectively, which AT 62.7% to 37.3% in the GC and AT were significantly higher than the GC content. 133 sequences were found in more than 155 polymorphic loci, including single polymorphic loci 53, 102 parsimony informative sites. 155 polymorphic loci, conversion takes place 143 times transversion 16 times, including four sites simultaneously transitions and transversions, the nucleotide variations mainly to the conversion. The 2.10 genetic diversity of sheep breeds found in more than 155 polymorphic loci identified 103 kinds haplotype, Lanzhou fat-tailed sheep and of Hanzhong Sheep and Sunite sheep each have a shared haplotype (Hap 3 0 and Hap 8 1). 10 sheep populations average nucleotide diversity: .01553 ± 0.00347; haplotype diversity: 0.947 ± 0.048; average number of nucleotide differences 20.532 ± 9.125. 10 varieties of nucleotide diversity performance a large difference in the range of variation: 0.00697 0 .02209 nucleotide diversity largest and smallest groups were Bashibai sheep (0.02209) and Zhaotong sheep (0.00697); single type diversity range of variation for 0.803 , 1 .000, haplotype diversity of the highest and lowest groups are the Taxkorgan sheep and Hanzhong sheep. Results, Zhaotong sheep and Hanzhong sheep genetic diversity than the poor, Bash thanks to sheep and the Taxkorgan sheep's genetic diversity is extremely rich Overall, 10 domesticated sheep breeds abundant genetic diversity of eight species of . 3.10 sheep populations interspecific and intraspecific genetic distance and genetic differentiation of the sheep populations interspecific and intraspecific genetic distances calculated using Kimura 2-parameter model in MEGA4.0 results: 10 sheep breeds The genetic distances 0.011 0 .031; minimum genetic distance between Zhaotong the sheep and taxkorgan sheep and Zhaotong sheep, with Bayinbuluke sheep, are 0.011; Zhaotong sheep introduced species genetic farthest 0.031 10 sheep breeds intraspecific genetic distance 0.007 0 .023. The intraspecific genetic closest and farthest varieties were Zhaotong sheep (0.007), and Bashibai sheep (0.023). 10 sheep breeds average genetic distance of 0.022. AMOVA analysis showed: the sheep genetic variation comes mainly from within the group. Genetic differentiation results from the 10 varieties of view, except for the obvious degree of differentiation of Bashibai sheep with the introduction of varieties, the remaining Chinese indigenous sheep breeds with the introduction of sheep breeds differentiation is obvious. 4.10 analysis of the origin and evolution of the sheep populations in the Kimura 2-parameter model, the 103 haplotype and five NCBI download sequence (three wild sheep sequences MEGA4.0 program, has been reported in an Asian sequence of type A and one type B sequence) of the phylogenetic tree, and Bootstrap test repeated 1000 times, results showed that: There were the sheep clustered into two branches, Mofo Lun sheep (AY091490) and one branch clustered into one group, Yuan sheep (AJ238300) and argali (AF039580) clustered into one group alone; Asia Type A (AF039578) and European type B (AF039577) were clustered into one group with two branches. The intermediary network diagram 103 haplotype build also a clear demonstration of the two developmental Group. Using MEGA downloaded in GenBank sequence of sheep, and in this study 133 sequences Construction NJ tree, the results show that these sequences are divided into three branches, the present pilot study sequences appear in the first and in the second branch, without appears in the third branch. This further illustrates: test of 10 sheep breeds exist two separate maternal origins Mofo Lun sheep is one of the ancestor of domestic sheep, and the the Yuan sheep and argali is not the ancestor of the domestic sheep. 5. Groups expansion analysis of the two branch nucleotide mismatch analysis shows two branches showed a single peak, and the value of the two branches Fu's Fs significantly negative (-24.91442 -19.19121) (P lt; 0.01), shows that the two lineages may have experienced population expansion.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Sheep
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