Dissertation > Excellent graduate degree dissertation topics show

Isolation and Identification of Antimicrobial Substance Produced by Bacillus Subtilis fmbJ

Author: JuYanJuan
Tutor: BieXiaoMei
School: Nanjing Agricultural College
Course: Fermentation Engineering
Keywords: B.subtillus fengycinB isolation and purification structural indentification 4" -isovaleryl-spiramycinⅢ
CLC: TQ920.6
Type: Master's thesis
Year: 2010
Downloads: 29
Quote: 0
Read: Download Dissertation

Abstract


Recently, an increasing number of people are focusing on exploring the natural antimicrobial substance which is broad spectrum antibacterial, safer and more stable. Natural antimicrobial substance can be divided into three kinds according to the sources originated from animal, plant and microorganism. Microorganism is becoming the predominant source for natural antimicrobial substance as they could be easily cultivated during a short period with low cost.At present, bacteriocin and lipopeptide produced by Bacillus subtilis had been throughly investigated and already been used in plant disease control and pharmacy industry. Several antimicrobial substances from Bacillus subtilis fmbJ have been identified by our lab. This study mainly focused on isolation and structural identification of natural antimicrobial substance from Bacillus subtilis fmbJ. This research provided theories about antimicrobial substance application in the food processing and preservation. The main conclusions are as follow:1. Establishing the isolation and purification methods of the antimicrobial substance. The crude extracts were isolated from the metabolic products using silica gel column chromatography and Sephadex LH-20. Then, the purified substance was obtained by preparative HPLC. In the process of silica gel column chromatography, the effect factors of mobile phase, sample load and flow rate were optimized. The optimal conditions were as below:flow rate 0.9 ml/min, sample load 1 ml, chloroform/methanol(V/V=1;97/3;19/ 1;93/7) as eluting solvent.2. The inhibitory test showed that the antimicrobial substance I exhibited a strong antagonistic activity against Penicillium meleagrinum. The antimicrobial substanceⅡinhibited gram-positive bacteria such as Staphylococcus aureus and Bacill us cereus.3. Identifying the antimicrobial substance I with method of ESI-MS. The result displayed that the molecular weight of antimicrobial substance I was 1534D, on base of the characteristic fragment m/z 994,1108, the antimicrobial substance I of m/z 1535 is identif- ed as FengycinB.4. Identifying the antimicrobial substance II by use of ES1-MS, IR and NMR. The result of IR indicated that the infrared spectrum of the antimicrobial substance II was similar to acetyl spiramycin. These data of 3350 cm-1(OH),1728 cm-1(CO),1580 cm-1 (HC=CH-CH=CH),1030 cm-1 and 1110 cm-1 (C-O-C) suggested that antimicrobial substance II has macrolide residues with the hydroxyl and the acyl group.ESI-MS demonstrated that the molecular weight of antimicrobial substance II was 982D. MS/MS analysis showed that the antibacterial substanceⅡshared the same characteristic fragment of m/z 755,596and 582 with acetyl spiramycinⅢ, which indicated that the both substances had the same structure.The 1H-NMR and 13C-NMR of the antimicrobial substances II were close to acetyl spiramycinⅢ. The proton signals and carbon signals of forosamine, mycaminose, isovalerylmycarose unit and macrolide residues could be easily found in the 1H-NMR and 13C-NMR. Summarizing all above analysis,the antimicrobial substancesⅡwas identified to be 4"-isovaleryl-spiramycin.

Related Dissertations

  1. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  2. Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS221
  3. Study on Antioxidant Activities and Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS202.3
  4. Angelica Purification and Houttuynia injection of qualitative and quantitative analysis,R284
  5. Rana skin separation and purification of the bioactive peptides and some of its biological activity,R284
  6. The Research on the Antioxidant Fat-Solube Compositions of Salicornia Herbacea L.,R284.1
  7. Study on Antioxidive Peptides from Fermented Soybean Meal,TQ464.7
  8. Fermentation, Isolation, Purification and Properties of Antifungal Active Substances Produced by Paenibacillus Polymyxa Cp-S316,S476
  9. Strain TR-20 purified ginsenoside into research and transformation products in Notoginseng separation,S567.236
  10. Preliminary Studies on Purification and Characterization of Chlorophyllase in Camellia Sinensis (L)O.Kuntze,S571.1
  11. Researches on Fermentation Conditions for the Production of Chloroperoxidase,Q93
  12. Study on Improving Efficiency of Bacteria for Biological Nitrogen Removal by Cell Fusion,X703
  13. Studies on Purification and Enzymatic Characteristics of Cholesterol Oxidase,Q814
  14. The Influence on the Function of the Islet Isolation and Purification in Rat by Fatty Infiltration in Pancreas,R657.5
  15. Effects of Ultra-High Pressure Treatment on G-actin of Bovine Skeletal Muscle,TS201.21
  16. Study on the Mechanisms of MCF-7 Cells Apoptosis Induced by Ganodema Lucidum Polysaccharide Containing Selenium SeGLP-2B-1,R737.9
  17. Study on Extraction Separation and Purification of Apigenin from Viola Yedoensis Makino,R284.1
  18. Isolation and Purification of Endophyte Fungus from Huperzia Crispata (Ching) and Study on Biologic Fermentation Technology of Potassium Hydrogen Oxalate Hydrate,S567.239
  19. Purification and Active Texting of Antimicrobial Peptides in Apostichopus Japonicus,Q51
  20. Research on Extraction, Purification and in Vitro Antitumor Activity of Polysaccharides from Porphyra Yezoensis,R730.5

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > General issues > Fermentation process
© 2012 www.DissertationTopic.Net  Mobile