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The Chronomodulated Radiosensitization by Cmna on Nasopharyngeal Carcinoma in Vivo

Author: ChenZuo
Tutor: LiShaoLin
School: Chongqing Medical University
Course: Oncology
Keywords: Glycodidazolum sodium Hour radiotherapy Hypoxic radiation sensitizers HIF-1α γ-H2AX
CLC: R739.63
Type: Master's thesis
Year: 2010
Downloads: 23
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Abstract


Objective: To observe the glycodidazolum sodium (CMNa) nasopharyngeal tumor-bearing nude mice hour radiation sensitizing tumor growth, HIF-1α, γ-H2AX and apoptotic protein expression levels in tumor tissue was detected to explore sodium glycodidazolum mechanisms of radiosensitization nasopharyngeal carcinoma xenografts hour. Methods: 130 Balb / c nude mice under uniform lighting conditions synchronize breeding, the establishment of a unified biological rhythm for at least three weeks. Establish a unified biological rhythms, poorly differentiated nasopharyngeal carcinoma cells (CNE-Ⅱ), at the outer side of the right thigh of nude mice inoculated establish nude mice nasopharyngeal xenograft model. Tumors and achieve inclusion criteria, 112 nude mice were randomly divided into three groups: radiotherapy (, betel quid, RT) group, RT CMNa group, blank control group, and each group four hour 3HALO (light after hours, hours after light onset), 9HALO 15HALO 21HALO divided into four subgroups. Which the RT CMNa group 3HALO, 9HALO, 15HALO, 21HALO four point in time before the one hour given intraperitoneal injection of sodium 20mg/kg glycodidazolum and RT group, RT CMNa group at four time points were single radiotherapy dose of 10Gy. Subgroup half nude mice were sacrificed 24 hours after the end of the hour radiotherapy for tumor specimens; remaining in nude mice was measured growth and extension of time (regrowth delay time, TGD), growth curve; using immunohistochemical methods to detect tumor specimens HIF-1α, γ-H2AX and apoptotic protein expression levels, and semi-quantitative analysis of immunohistochemical image analysis software. In this study, using completely randomized single-factor analysis of variance on the immunohistochemical detection results of each group were statistically analyzed. Results: processing factors are different degrees of inhibition of nasopharyngeal carcinoma xenografts in nude mice. By the comparison of each group TGD best to the RT CMNa group inhibitory effect on the tumor. In the group TGD: 15HALO gt; 21HALO gt; 9HALO gt; 3HALO 15HALO with 3HALO of TGD was statistically significant (P lt; 0.05). Immunohistochemical detection of tumor specimens (PI value): HIF-1α protein expression levels of γ-H2AX and apoptosis was time rhythm, while Chen's radiosensitizer group and radiotherapy group between HIF- 1α, γ-H2AX and apoptotic protein expression differences were statistically significant (P lt; 0.05). The expression of HIF-1α in the RT CMNa group and RT group: 3HALO gt; 9HALO gt; 21HALO gt; 15HALO of 15HALO with 3HALO HIF-1α expression level of statistical significance (P lt; 0.05). expression of γ-H2AX in the RT CMNa group and RT group: 15HALO gt; 21HALO gt; 9HALO gt; 3HALO of 15HALO with 3HALO γ-H2AX expression level of statistical significance (P lt; 0.05). Apoptotic protein expression in the RT CMNa group and RT group: 15HALO gt; 21HALO gt; 9HALO gt; 3HALO the of which 15HALO with 3HALO, apoptosis protein expression level of 9HALO statistically significant (P lt; 0.05). Conclusion: glycodidazolum sodium Joint hour obvious hour radiosensitizing effect of radiotherapy for nasopharyngeal carcinoma xenografts in nude mice, tumor inhibitory effect of 15HALO RT CMNa group. Confirmed the the sodium glycodidazolum radiosensitizing effect of tumor hour time rhythm, its inhibitory effect on tumor immunohistochemistry results have good consistency. Its mechanism may be related to HIF-1α expression, DNA double-stranded injury, apoptosis.

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CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
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