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Cell gap junction protein Cx26 and Cx43 gene in bladder transitional cell carcinoma invasion and proliferation activity of

Author: TanWeiFa
Tutor: ZengXiangYang
School: Nanhua University
Course: Surgery
Keywords: Cx26 Cx43 T24 bladder cancer cell lines Attack Proliferation
CLC: R737.14
Type: Master's thesis
Year: 2010
Downloads: 20
Quote: 0
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Abstract


[Objective] To construct the cell gap junction protein Cx26 and Cx43 gene plasmid and transfected into T24 bladder transitional cell carcinoma , and its expression was observed after the invasion of cancer cells and proliferation activity. [Method] Using primer premier5.0 software design primers by PCR method doubled the cell gap junction protein Cx26 and Cx43 gene electrophoresis. Restriction endonuclease digested eukaryotic expression vector pEGFP-N3, Cx26 and Cx43 gene underwent genetic recombination, the Cx26 and Cx43 gene is inserted into the multiple cloning sites of pEGFP-N3 on . Cx26 and Cx43 gene containing plasmid transfected T24 bladder cancer cells , LB medium for the growth of plants after selection , green fluorescence and Western blot and expressed successfully transfected cells were cultured after artificial basement membrane invasion and proliferation assays , through count number of cells expressed through Matrigel invasion , MTT experiment measuring the absorbance indicates cell proliferation . [Results] 1 . Successfully constructed cell gap junction protein Cx26 and Cx43 gene plasmids . 2 transfected with Cx26 or Cx43 gene in bladder cancer T24 cells underwent artificial basement membrane invasion experiment , and only with the T24 cells transfected with pEGFP-N3 of T24 cell invasion compared with significantly reduced. 3 transfected Cx43 gene Cx26 gene or T24 bladder cancer cell line MTT assay showed that , at different times , and only with the T24 cells transfected with pEGFP-N3 of T24 cells compared to their proliferative capacity decreased significantly. [Conclusion] can be constructed cell gap junction protein Cx26 and Cx43 gene plasmids ; Cx26 or Cx43 gene expression may inhibit bladder cancer cell invasion and cell proliferation effect.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Bladder tumor
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