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Objective: To investigate the intradural local infusion of the protective effect of MP on spinal cord injury, to provide theoretical guidance for clinically localized perfusion MP treatment of spinal cord injury. Methods: 72 New Zealand white rabbits were randomly divided into four groups: I local perfusion group, damage control group II, III intravenous group, Ⅳ blank control group. To cause rabbit incomplete paralysis model, subdural catheter, local infusion group in the immediate postoperative modified Allen's method 60.0 gcm force impact T8 segmental spinal cord, 6h, 12h, 18h injected methylprednisolone MP (1mg/kg dubbed the lml solution, four injection), injury control group at the same point in time, given the volume of normal saline. Intravenous group of successful modeling did not mention the pipe, and 30mg/kg first dose intravenous administration time after injury 1h ie 5.4mg/kg/h calculate 23h total, four intravenous injection. Blank control group only open lamina subdural catheter, not injured, not administration. Hour before injury, 1 h after injury, 24h, 7d, 14d with modified Tarlov score motor function score; 6 h after injury, 24h, 48h, 72h, blood was centrifuged spectrophotometer SOD and MDA absorbance detection its content and change; injury after 24h, 7d, 14d three time points observed injury spinal cord histological changes (HE staining) and neural cell apoptosis (TUNEL staining). The resulting data were analyzed using SPSS16.0 statistical software. Results: 1. Motor function score Ⅰ, Ⅲ group neurological recovery was better than group Ⅱ, Ⅲ group was better than group Ⅰ. After spinal cord injury, SOD levels reduce MDA levels (P lt; 0.05); Ⅰ group III SOD levels significantly higher than that in group Ⅱ, group Ⅲ SOD levels significantly higher than those in group Ⅰ; Ⅰ group III MDA levels and increase in group Ⅱ, Ⅲ group was significantly lower than the level of MDA and the increase was significantly lower than in group Ⅰ (P lt; 0.05). 3. Ⅰ, Ⅱ, Ⅲ group were axonal swelling, liquefaction necrosis, cavity formation and gliosis, but more serious group Ⅱ. Ⅰ, Ⅲ group of TUNEL-positive cells rate was significantly less than group Ⅱ, Ⅲ group of TUNEL-positive cells rate less than group I (P lt; 0.05). Conclusion: intradural local perfusion and intravenous MP in the treatment of SCI, could promote the recovery of neurological function; increase the concentration of SOD, reduce the concentration of MDA, reduce free radicals and lipid peroxidation in spinal cord injury; reduced spinal cord injury partial apoptosis, inhibition and reduce cell necrosis after spinal cord injury. But less than the intravenous regional perfusion intradural MP efficacy may be related to the size of local perfusion, pending further study.
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