|
Objective: cell transplantation is an important research direction in spinal cord injury treatment. Rabbit Schwann cells in rats after half cross section of spinal cord injury, transplant immune isolation effect microcapsules, by observing the rat alkaline into the expression of fibroblast growth factor and hind limb motor function changes, explore microencapsulated Schwann cells transplantation for spinal cord regeneration. Methods: under sterile conditions to take the rabbit sciatic nerve Schwann cell suspension into the legal system, and then the gas is injected into the legal system into Schwann cells microencapsulated double enzyme digestion. Empty sac Schwann cells with the prepared package. 80 SD rats were randomly divided into four groups: the cell group (N = 25), the empty capsule group (N = 25), microcapsules group (N = 25) and normal group (N = 5). Among them, the first three sets of the establishment of the first 10 thoracic spinal cord the right half cord injury model, respectively gelatin sponge implantation damage at the adsorption 10μL Schwann cell suspension (cell group), 10 μL of empty capsule (empty capsule group), 10μL Schwann cells microcapsules (microcapsules group,); normal group without any intervention. BBB rated Determination of hindlimb motor function recovery and production of paraffin sections of the spinal cord observed in experimental animals. 1,3,7,14,28 d after 4% paraformaldehyde in rats, the cell group, empty capsule group, microencapsulated interception injury level spinal cord; normal group take the corresponding parts of the spinal cord, four groups of rat spinal cord organizations are made of paraffin sections. Slice by HE staining and Nissl staining for pathological examination, and do immunohistochemical staining and image analysis observed basic fibroblast growth factor expression changes in the spinal cord injury site. Results: After modeling rat right hind limb instantly come to a standstill BBB Rated for O. Postoperative recovery of hind limb motor function of the animals in each group have different levels over time, but did not reach normal levels. Days 7,14,28 mc group hindlimb motor function recovered rapidly after transplantation, BBB score better than the cell group and the empty capsule group (P lt; 0.05 or P lt; 0.01). Pathological examination, and the first 3 days after transplantation, each group of spinal cord injury area have a large number of macrophage and leukocyte infiltration, while the proliferation of glial cells, but with the cell group, the empty capsule group, the microencapsulated cell infiltration to a lesser extent, gum stromal cell proliferation significantly. Materials transplant after 7 days, a large number of microencapsulated gliosis, with no white blood cells and macrophage infiltration, cell groups, empty capsule group still leukocyte and macrophage infiltration. Basic fibroblast growth factor-positive cells expressed mainly seen in the cytoplasm of neurons and glial cells in the nucleus. 1, 3 and 7 days after transplantation, glial cells, mainly in the vicinity of the spinal cord injury at the expression of three days maximum expression level; 14 days in the cytoplasm of neurons within the visible expression of basic fibroblast growth factor, microencapsulated expression level was significantly higher than the cell group and the empty capsule group (P lt; 0.01); expression levels decreased in 28 days. Conclusion: microencapsulated rabbit Schwann cells transplanted into the injured rat spinal cord, can suppress immune rejection after xenotransplantation, reduce inflammation, increase the expression of basic fibroblast growth factor to promote the restoration of hind limb function and spinal cord regeneration
|