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1.HIFU: To investigate the purpose of killing effect of rabbit VX2 breast cancer, HIFU (High Intensity Focused Ultrasound, HIFU) rabbit VX2 breast microvascular density (microvessel density, MVD), micro-lymphatic vessel density (lymphatic microvessel density, LMVD in) and proliferating cell nuclear antigen (proliferating cell nuclear antigen, PCNA) impact. Methods: the rabbit VX2 breast cancer, model 30, were randomly divided into a control group of 5, HIFU treatment group 25. Respectively before treatment, three days after the treatment, color Doppler flow imaging (CDFI) and contrast-enhanced ultrasound (CEUS) examination, analysis of tumor perfusion changes before and after HIFU treatment. By immunohistochemical staining of tumor tissue the MVD, LMVD and PCNA expression. Results: After HIFU treatment, CDFI was not detected in the target area and any color flow signal; CEUS examination found the 21 rabbit tumor tissue contrast agent filling, four linear echogenic. Observed under the microscope 400 magnification, HIFU group MVD average of 2.3 ± 0.56, LMVD an average of 1.7 ± 0.78, PCNA positive rate was 0, and the control group MVD LMVD in PCNA difference between statistical significance (P lt; 0.05 ). Conclusion: HIFU can completely kill breast cancer cells and destroy tumor metastasis ways to cure the effects of breast cancer. 2 CEUS sentinel lymph purpose: to explore the prospects of contrast-enhanced ultrasound detection of sentinel lymph nodes (sentinel lymph node, SLN) compared with methylene blue (Methylene blue, MB) tracer method. Methods: Rabbit VX2 model of breast cancer 24 subcutaneous injection of SonoVue, contrast-enhanced ultrasound imaging SLN, to observe the contrast-enhanced; tracer injected subcutaneously MB; axillary lymph node dissection resection SLN parallel AE1/AE3 immunohistochemical staining . Results: 24 tumor rabbit ultrasound contrast detection echo enhanced uniformity SLN 12 and SLN 22 echo uneven enhancements and partial filling defect; the MB tracer method detection SLN 32; axillary lymph node dissection intraoperative SLN 38 a. Surgical resection of the SLN immunohistochemical results for the standard ultrasound contrast detection rate (89.47%), sensitivity (95.24%) and MB tracer method detection rate (84.21%) and sensitivity (91.30%) significant differences (P gt; 0.05); but its specificity (84.62%) and accuracy (91.18%) was significantly higher than that the MB tracer method specificity (26.67%) and accuracy (65.79%) (P lt; 0.05). Conclusion: by subcutaneous injection of ultrasound contrast agent for imaging breast cancer SLN MB tracer method has some advantages, is expected to become a common method of detection of SLN, SLN lay the foundation for HIFU treatment of breast cancer.
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