Dissertation > Excellent graduate degree dissertation topics show

The Correlation between the Expression Level of the Nuclear Factor-κB in Peripheral Blood Mononuclear Cells and the Airflow Limitation in Patients with Chronic Obstructive Pulmonary Disease

Author: SongShan
Tutor: ChenShaoPing
School: North Sichuan Medical College
Course: Internal Medicine
Keywords: Chronic obstructive pulmonary disease NF-κB Interleukin-8 Interleukin-10
CLC: R563.9
Type: Master's thesis
Year: 2010
Downloads: 38
Quote: 0
Read: Download Dissertation

Abstract

Objective: Chronic obstructive pulmonary disease (COPD) due to its large number of sick, high mortality, the burden on the social and economic weight, has become a major public health problem. 4 COPD largest cause of death, the World Bank / World Health Organization (WHO) announced that COPD will be among the first five of the world's burden of disease by 2020. In our country, COPD is still a serious harm to the health of people with chronic respiratory diseases, recent population survey of some regions of China, the prevalence of COPD accounted for 8.2% of the population over the age of 40. The pathogenesis of COPD is not yet clear, well-recognized airway inflammation in COPD pathogenesis. Single nuclear factor-κB (NF-κB) is involved in a variety of inflammatory cells, immune cells, gene transcription, regulating the role of inflammation and immune responses. The study by the level of NF-κB in peripheral blood mononuclear cell nucleus and heavy - very severe AECOPD patients with glucocorticoid treatment of peripheral blood mononuclear cell nucleus level of NF-κB study in patients with acute exacerbation of COPD (AECOPD) and peripheral blood mononuclear cell nucleus level of NF-κB and serum interleukin-8 (IL-8), leukocyte interleukin -10 (IL-10) content compared to peripheral blood mononuclear cell nucleus level of NF-κB and COPD lung function in patients with correlation analysis to investigate the role of NF-κB in the pathogenesis of COPD and its relationship with the COPD disease severity, as well as the possible mechanisms of glucocorticoid treatment of COPD. : AECOPD inpatients observed object and set it to a total of 60 cases of the observation group, based on the severity of the disease is divided into two subgroups: mild - moderate group (30 cases), heavy - very severe group of 30 patients, the basic treatment the same way (anti-infection, oxygen, antispasmodic asthma, cough symptomatic treatment), and - very severe group of patients with glucocorticoid treatment on the basis of the basic treatment plus. The same period in the outpatient healthy individuals as a control group of 30 patients. Were taken light - moderate the AECOPD group of patients, re-- the the AECOPD group of patients before and after treatment and a control group of healthy volunteers were extremely severe peripheral anticoagulant tube at 4 ℃. Detected by Western blotting (Western blotting) levels of NF-κB in mononuclear nucleus, detected by ELISA, IL-8, IL-10 content, and COPD underwent pulmonary function testing (measured FEV1 percent predicted value%, FEV1/FVC value). Results: 1 AECOPD patients 1.1 AECOPD patients peripheral blood mononuclear cell nucleus changes of NF-κB in peripheral blood mononuclear cell nucleus NF-κB levels (4.37 ± 1.89) gray scale, higher than the normal control group, peripheral blood mononuclear nucleus NF grayscale than-κB levels (1.70 ± 0.39), the difference was statistically significant (P lt; 0.01). 1.2 weight - very severe group AECOPD patients peripheral blood mononuclear cell nucleus NF-κB level gray scale (5.87 ± 0.28), higher than the light - moderate group AECOPD patients peripheral blood mononuclear cell nucleus NF-κB levels (2.75 ± 0.95 ) grayscale than the difference between the two groups was statistically significant (P lt; 0.01). 1.3 weight - very severe group of patients with AECOPD glucocorticoid treatment on peripheral blood mononuclear cell nucleus NF-κB level gray scale (2.52 ± 0.42), lower than the pre-treatment of the group of patients with peripheral blood mononuclear cell nucleus level of NF-κB gray scale (5.87 ± 0.28), the difference was statistically significant (P lt; 0.01), but still higher than the level of NF-κB in peripheral blood mononuclear cell nucleus normal control group (1.70 ± 0.39) gray than , the difference was statistically significant (P lt; 0.01). 2.1 AECOPD patients 2 AECOPD patients with serum IL-8 changes of serum IL-8 content (49.18 ± 13.96) pg / ml, higher than the normal control group, serum IL-8 content (35.87 ± 6.35) pg / ml difference between the two groups has statistically significant (P lt; 0.05). 2.2 weight - very severe group AECOPD patients of serum IL-8 content (52.89 ± 10.97) pg / ml, higher than light - moderate group of patients with AECOPD of serum IL-8 content (40.99 ± 5.54) pg / ml difference between the two groups has statistically significant (P lt; 0.05). 2.3 weight - very severe group of patients with AECOPD glucocorticoid treatment serum levels of IL-8 content (40.87 ± 5.97) pg / ml, lower than the pre-treatment of the group of patients with serum IL-8 levels (52.89 ± 10.97) pg / ml, two group, the difference was statistically significant (P lt; 0.05), compared with the normal control group, serum IL-8 levels (35.87 ± 6.35) pg / ml, the difference was not statistically significance (P gt; 0.05). 3.1 AECOPD 3 AECOPD serum IL-10 changes of serum IL-10 content (82.91 ± 3.83) pg / ml, lower than the normal control group, serum IL-10 levels (148.72 ± 2.52) pg / ml difference between the two groups has statistically significant (P lt; 0.01). 3.2 weight - very severe group AECOPD patients of serum IL-10 content (52.98 ± 1.30) pg / ml, below the light - moderate group AECOPD serum IL-10 levels (113.68 ± 2.36) pg / ml difference between the two groups has statistically significant (P lt; 0.01). 3.3 weight - very severe group AECOPD patients by sugar corticosteroid therapy serum IL-10 levels (102.00 ± 2.88) pg / ml, higher than the pre-treatment of the group of patients with serum IL-10 levels (52.98 ± 1.30) pg / ml, two group difference was statistically significant (P lt; 0.01), but still lower than the normal control group, serum IL-10 levels (148.72 ± 2.52) pg / ml, and the difference was statistically significant (P lt; 0.01). 4 AECOPD patients with peripheral blood mononuclear cell nucleus NF-κB levels and serum IL-8, IL-10 content in patients with AECOPD peripheral blood mononuclear cell nucleus NF-κB levels increased with the increase in serum IL-8 levels were positively correlated The correlation coefficient of r = 0.84. AECOPD patients with peripheral blood mononuclear cell nuclear NF-κB levels with serum IL-10 levels increased and decreased, a negative correlation, the correlation coefficient r = -0.78 5 AECOPD patients with NF-κB levels in the peripheral blood mononuclear cell nucleus and lung function correlation AECOPD patients with peripheral blood mononuclear cell nucleus NF-κB correlated with FEV1 percentage is expected to decline in value% increase, and a negative correlation, the correlation coefficient r = -0.97. Conclusion: NF-κB inflammatory response involved in AECOPD is a reflection of AECOPD severity of illness indicators, is an important part of AECOPD waterfall inflammatory response. 2, IL-8 involved in the AECOPD inflammatory reaction, by the regulation of NF-κB in inactive cytosolic NF-κB by inflammatory injury or immunological stimulation after entering the start of IL-8 gene transcription in the nucleus, promote IL -8 expression, but its specific adjustment process has yet to be further explored. 3, IL-10 can inhibit the inflammatory response of AECOPD involved in the inhibition of AECOPD inflammatory response through the inhibition of NF-κB in other ways. 4, glucocorticoids inhibit airway inflammation by promoting the inhibition of inflammatory cytokine IL-10 expression, thereby inhibiting NF-κB and IL-8 expression, to control the airway inflammation of COPD. In addition glucocorticoid inhibition of the inflammatory response may also participate in a variety of links, yet to be further explored.

Related Dissertations

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Other
© 2012 www.DissertationTopic.Net Mobile