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Analysis on Genetic Character of Type Ⅰ Poliovirus in China in 2009

Author: AnJunJing
Tutor: XuWenBo
School: Disease Control and Prevention Center
Course: Immunology
Keywords: Type 1 polio virus VP1 coding region of the gene characteristics Real - time fluorescence quantitative Reverse transcription - polymerase chain reaction Identification of type within
CLC: R512.4
Type: Master's thesis
Year: 2010
Downloads: 40
Quote: 0
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Abstract


Poliomyelitis (polio) is an acute infectious disease that occurs mainly in children can be inactivated poliovirus vaccine (Inactivated Poliovirus vaccine, IPV) or oral polio live attenuated vaccine (Oral Poliomyelitis Attenuated Vaccine, OPV) to prevent it. Global polio eradication program began in 1988, the type Ⅱ global spread of wild poliovirus has been interrupted in 1999; the end of 2009, in addition to the four countries, Afghanistan, India, Nigeria, Pakistan, and type Ⅰ and Ⅲ indigenous wild polioviruses The spread of the virus has also been blocked. China since 1995, found no indigenous wild poliovirus in 2000, including China, the World Health Organization (WHO) Western Pacific (WPRO) declared a polio-free state, China is currently still to use OPV maintain polio-free status. Replication in the human gut OPV strains mutate, some variation can lead to strong neurovirulence Reply cause of vaccine-associated polio (VAPP). The unvaccinated population will be served the Miao who excretion vaccine virus to the variability in the outside environment, and caused its circulation loop to a certain extent (VP1 coding region nucleotide mutation rate ≥ 1%) in the crowd, the spinal cord is called polio vaccine-derived virus (VDPV). Although China is currently in a polio-free state, but because of the wild poliovirus type Ⅰ popular still in individual countries and regions, so there is still the risk of wild poliovirus importation. 57 Ⅰ maintain polio-free status for our country in order to study the genetic characteristics of workers poliovirus (PV), provide the basis for isolated case surveillance system in 2009 Acute Flaccid Paralysis (Acute Flaccid Paralysis, AFP) VP1 coding region of the type of PV by reverse transcription - polymerase chain reaction (Reverse Transcription Polymerase Chain Reaction, RT-PCR) method for amplification, and PCR products were sequenced and analysis, not found in the PV field strains or vaccine-derived PV; mutant but found 5 plant height, which separated the two AFP cases plant height mutant nucleotide sequence homology of 100%. While 57 type I PV VP1 region sequence than the results show that the two nucleotides (nt2747 and nt2749) mutational hotspots. Jiangxi Province and Hubei Province plant height mutant epidemiological and laboratory test results can not be ruled out they the short cycle need to be in-depth study. Meanwhile, the presence of the mutation hotspot Step strains by selection pressure easily reply mutation into the wild type, resulting in a series of neurovirulence phenotype changes. World Health Organization recommended by the U.S. Centers for Disease Prevention and Control Center developed real-time quantitative reverse transcriptase (Reverse Transcription, RT) PCR 57 type I PV six representative strains type within identification and vaccine-derived poliovirus ( Vaccine-derived Polioviruses, VDPVs) screening, comparative analysis and test results with the strains of VP1 nucleotide sequence of measurement results. The results show that the real-time quantitative RT-PCR unidentifiable virus 6 5 Pre-VDPVs new internal identification methods used as recommended by WHO, the real-time quantitative RT-PCR method is applicable in China PLN (poliomyelitis laboratory network) retrospective and prospective studies of large sample polio virus has yet to be real-time quantitative RT-PCR method.

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CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Poliomyelitis ( polio )
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