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Objective To evaluate the expression of CD49d, CCR9, CD62L on T lymphocytes and co-receptor CCR5 on CD4+ T lymphocytes in AIDS patients before and after HAART,and furthermore to explore its clinical significance.Methods The study was performed in 42 cases of AIDS patients and 18 cases of healthy controls. The expression of CD49d, CCR9, CD62L on CD3+, CD4+ , CD8+T lymphocytes and CCR5 on CD4+ T lymphocytes, and CD45RO on CD4+CCCR9+ and CD4+CCR5+ T lymphocytes in AIDS patients and healthy controls were analysed by Flow cytometry. Software BD FACSDiva was used to calculate the percentage of expression,then study the changes and significance between untreated group and therapy group .Results Compared with therapy group , the frequency of CD3+CD49d+, CD3+CCR9+,CD3+CD62L+, CD4+CD49d+, CD4+CCR9+,CD4+CD62L+, CD8+CD49d+, CD8+CD62L+, CD4+CCR9+CD45RO+,CD4+CCR5+CD45RO+ T lymphocytes in untreated group were significantly decreased, while the frequency of CD4+CCR9+CD45RO - , CD4 + CCR5+, CD4+CCR5+CD45RO - T lymphocytes significantly increased; compared with controls, the frequency of CD3+CD49d+, CD3+CCR9+, CD3+CD62L+, CD3+CD4+ , CD4+CD49d+, CD4+CCR9+, CD4+CD62L+ , CD4+CCR9+CD45RO+, CD4+CCR5+CD45RO+ T lymphocytes in untreated group were significantly decreased, while the frequency of CD4+CCR9+CD45RO-, CD4+CCR5+, CD4+CCR5+CD45RO-T lymphocytes significantly increased; compared with controls, the frequency of CD3+CD4+,CD3+CCR9+, CD4+CD62L+, CD8+CCR9+, CD8+CD62L+ T cells in therapy group were significantly decreased,while the frequency of CD3+CD8+,CD4+CCR5+ T lymphocytes significantly increased.Conclusion The expression of gut homing receptor molecule CD49d, CCR9 and lymph node homing molecule CD62L, co-receptor molecule CCR5 in AIDS patients on T lymphocytes was impaired; HAART can partially reverse this immunologic pathological phenomena; CD49d, CCR9 and CD62L may be suggested to indicate the progression of AIDS and immunologic reconstitution after HAART.
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