Dissertation > Excellent graduate degree dissertation topics show

The Inhibiting of SIRNA on VP1 Expression in Human Cell

Author: LvYuChun
Tutor: ChenQuan
School: Chongqing Medical University
Course: Immunology
Keywords: VP1 gene RNAi siRNA Attenuated Salmonella
CLC: R392
Type: Master's thesis
Year: 2010
Downloads: 69
Quote: 0
Read: Download Dissertation

Abstract


AIM: To obtain the SiRNA which can efficiently inhibit the expression of VP1 of EV71 virus steadily; and to transform it into attenuated Salmonella to construct a oral gene vaccine targeting to VP1 gene of EV71, then to provide the theoretical and experimental basis for targeting treatment of hand-foot-mouth disease with RNA interference technology。METHODS: By using molecular cloning technique, the specific SiRNA interference plasmids targeting to VP1 mRNA were constructed based on the pSEB-HUS vector which has EGFP maker, U6 and H1 promoters, which were confirmed by double-enzyme digestion and DNA sequencing. After these plasmids were transinfected into A549 cell respectively, the expression level of vp1 mRNA and protein was detected by Q-PCR, Western blot and fluorescence microscope, in order to screen the clones in which VP1 was steadily inhibited.RESULTS: The SiRNA interference plasmids (pSVsi1, pSVsi2 and pSVsi3) were successfully constructed. The expression level of VP1 mRNA and VP1 protein of pSVsi1 group decreased significantly and the inhibition rate were 90% and 89% respectively. The selected SiRNA plasmid pSsi1 was contructed and transformed into the attenuated Salmonella successfully.CONCLUSION: The specific SiRNA interference plasmids targeting to VP1 gene mRNA is successfully designed and constructed. The SiRNA which can efficiently inhibit the expression of VP1 of EV71 steadily is obtained, which provides the experimental models for developing EV71 vaccine in further study.

Related Dissertations

  1. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  2. Toxicities of Several Newly-Typed Insecticides to Colorado Potato Beetle, Leptinotarsa Decemlineata (Say) and Molecular Cloning of Two Kinds of Targets,S435.32
  3. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  4. RNAi Silencing of the TTF-1 Gene Induces Apoptosis in the A549 Lung Cancer Cell Line,R734.2
  5. Brain myocarditis virus VP1 fragment gene of stability of the , prokaryotic expression and the Applied Research,S852.65
  6. The Role and Mechanism of SDF-1/CXCR4 Axis in Small Cell Lung Cancer Invasion and Metastasis,R734.2
  7. Effects of siRNA Inhibit Class I PI3K on Humangastric Cancer SGC7901 Cells,R735.2
  8. Effect and mechanism in pediatric neuroblastoma organ-specific metastasis of CXCR4 expression,R739.4
  9. The Effect of Treatment from Dual Target in Murine Breast Tumors by Survivin and Vegf-c,R737.9
  10. Research FADD gene silencing mechanism of apoptosis in Parkinson 's disease on the,R742.5
  11. Study of Apoptosis in MCF-7 and Its Mechanism Caused by VEGF siRNA Transfected into It,R346
  12. The Expression of Stathmin in Ovarian Cancer and the Effect on Chemosensitivity to Cisplatin in Ovarian Cancer Cells,R737.31
  13. EPPIN gene polymorphism and low expression on male reproductive function,R698.2
  14. The Preliminary Analysis of Differential Expression Genes in the Development of Esophageal Carcinoma,R735.1
  15. The Expression and Role of SOX2 in Esophageal Squamous Cell Carcinoma,R735.1
  16. DNA Vaccine of Avian Reovirus σC Gene Delivered by Attenuated Salmonella Typhimurium and Its Immunogenicity,S852.659.4
  17. Preliminary Study on the Inflammatory Mechanism of Brucella Omp25 to Embryonic Trophoblast Cells,S852.61
  18. Construction of Lentiviral Vector siRNA of APP695 Gene and Its Effect on the APP695 Gene Expression in SH-SY5Y Cells,R346
  19. Inhibition of siRNA on the Expression of Cytoglobin Gene in Rat Hippocampus Cell in Vitro,R329
  20. Influence of Silencing NF-kB p65 by RNA Interference on BSEP and NTCP Expression in Estrogen Effected of Human Hepatic Cells,R329
  21. The Role of Stress PKCs on the Transition from Cardiac Hypertrophy to Heart Failure,R541.6

CLC: > Medicine, health > Basic Medical > Medical Immunology
© 2012 www.DissertationTopic.Net  Mobile