Dissertation > Excellent graduate degree dissertation topics show

The Research of Chemical Components and Functions of Essential Oil of Rosa Damascena

Author: YingLiYa
Tutor: SuPing
School: Zhejiang University
Course: Food Processing
Keywords: Rosa damascene essential oil chemical composition antibacterial extract antidepressant
CLC: TQ654.2
Type: Master's thesis
Year: 2012
Downloads: 658
Quote: 1
Read: Download Dissertation

Abstract


Rose essential oil has antibacterial, antioxidant effect, antiallergic effect, moisturizing effect. It can also promote cells regeneration and have good effect on respiratory system, digestive system, circulation system and reproductive system. Apart from that, rose essential oil can help reducing pressure, easing tension, promoting sleep and prevent neurological disease such as depression, anxiety and so on. It has wide application prospects in cosmetics industry, food industry and pharmaceutical industry. This paper mainly studies the steam distillation in Rosa damascena,which planted in Huzhou prefecture. The components of Rosa damascena essential oil were extracted by HS-SPME, Steam distillation, including coeno-water distillation and water and steam distillation were analysed by GC-MS. At the same time, the differences of the volatile composition of Rosa damascena were also contrasted. In this paper, Rosa damascena essential oil’s bacteriostatic action and antidepressant action were studied too, which aimed at providing a reference basis for application of Rosa damascena and its essential oil. The main research results are as follows:1.The effect of distillation time, material to liquid ratio, concentration of sodium chloride and pretreatment of Rosa damascena on the extraction rate of Rosa damascena essential oil were researched by single factor experiment. On the basis of single factor experiment, the Rosa damascena essential oil extracted by Steam distillation process were optimized by using of orthogonal optimization experiment. Optimization of process parameters for Rosa damascena essential oil was mixing the whole flowers and 5 times distillation liquid contained 15% sodium chloride and distilling 5 hours. The finally extracted rate of Rosa damascena essential oil was 0.0799%.2.The chemical composition of Rosa damascena essential oil extracted by HS-SPME, Steam distillation including coeno-water distillation and water and steam distillation were analysed by GC-MS.45,55and48 chemical components were respectively identified in the above three methods and the percentage content of them was respectively 96.34%,96.17% and 96.22%.6-Octen-1-ol,3,7-dimethyl-, (R)-was the highest content in three methods.3.87 chemical components were identified in Rosa damascena essential oil extracted by HS-SPME, Steam distillation including coeno-water distillation and water and steam distillation,19 chemical components could find in all three methods. According to a contrast and analysis, the author found that the chemical composition and percentage of content was quite different. The major chemical components of Rosa damascena essential oil extracted by HS-SPME were tepenes, alcohols and aldehydes, respectively accounting for 29.20%,49.10% and 7.64% of the total amount of rose essential oil. The major chemical components of Rosa damascena essential oil extracted by coeno-water distillation were alcohols, esters and alkanes, respectively accounting for 46.39%、25.81% and 16.77% of the total amount of rose essential oil. The major chemical components of Rosa damascena essential oil extracted by water and steam distillation were alcohols, esters, alkanes and terpenes, respectively accounting for 47.93%、19.98%、13.74% and 9.05% of the total respectively accounting for rose essential oil.4.The in vitro antibacterial effect of Rosa damascena essential oil was studied. The bacteriostasis function of rose essential oil on Eschirichia coli, Staphylococcus aureus and Salmonella typhimrium were tested by filtering paper method and tube double dilution method. The determination of diameter of inhibiting Eschirichia coli, Staphylococcus aureus and Salmonella typhimurium were 17.21,16.14,17.43mm, which belonged to moderate sensitive. The method of doubling deletion in test tube was used to detect the minimal inhibitory concentration (MIC) and minimal bactericidal concentration. The MICs of Rosa damascena essential oil on Eschirichia coli, Staphylococcus aureus and Salmonella typhimurium were all 1.25μl/ml, and the MBCs on three tested bacteria were 1.25μl/ml,2.5μl/ml,1.25μl/ml. Bacteriostatic experiment proved that Rosa damascena essential oil did not only have the bacteriostatic effect, but also have sterilization effect at certain concentration. These results show that Rosa damascena essential oil have great potential application in the prevention and treatment of disease caused by bacteria.5.Experimental model mice of depression were induced by reserpine for observing the correlation of the drug with Rosa damascena essential oil through olfactory system, including the degree of ptosis and body temperature. The results showed that there were different antagonisms in the degree of ptosis and body temperature. In reserpine model, there was no significant difference in antagonist mice hypothermia, however, there was significant difference in antagonist mice the degree of ptosis after building model 4 hours. Rose essential oil may play a better role in the long-term antidepressant effect. Independent activity test showed that rose essential oil could significantly inhibit the mice aromatherapy independent activities, which suggested that rose essential oil might restrain central nervous system. Furthermore, there were no statistical differences in antagonist mice the degree of ptosis among the high dosage group, middle dose group and the low dosage group. Whereas, the middle dose group and the low dosage group exhibited an excellent antagonism to mice hypothermia which induced by reserpine. In preliminary, it can predict that rose essential oil has the potential of preventing depression.

Related Dissertations

  1. Study on the Syntheses and Antibacterial Acticity of Metal Organic Complexes Built from Three Carboxylate Ligands,O621.13
  2. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  3. Properties and Application Research of Multi-Antibacterial Polyamide Fibers,TQ342.1
  4. Distribution of the Contents of Pectin and Cellulose and Their Effect on Smoking Quality in Flue-cured Tobacco of China,S572
  5. Study on the Dynamic Growth and Components Accumulation of Lavadula Angustifolia,S573.9
  6. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  7. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  8. The M Company Essential Oil Skincare Marketing Strategy Study,F274
  9. Study of Isolation and Identification on Antimicrobial Substances Produced by Lactobacillus Acidophilus NX2-6,TS201.3
  10. Study on Key Technology Optimization of Flue-cured Tobacco by Bulk Curing,TS44
  11. Effects of Climate Factors for Different Transplanting Dates on Quality and Ripe Tissue in Flue-cured Tobacco,S572
  12. Region Division and Quantitative Characteristics of Climate Factors for Qujing Tobacco-growing Areas,S572
  13. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  14. Fundamental Studies on the Chemical Constituents of Brassica Rapa L.seed.,R284
  15. Study on the Enrichment of Antimicrobial Peptides and Purification Technology in Wheat Germ,TQ936.16
  16. More than enough calcium and magnesium metal ligand complexes Synthesis, Structural Characterization and Antibacterial Activity,O641.4
  17. Schiff base Synthesis, Characterization and Antibacterial Properties,O641.4
  18. Dark purple Fritillaria medicinal active ingredient extraction technology and the chemical composition of,TQ461
  19. Preparation of Mesoporous Silica Inorganic Antibacterial and Its Application on Cotton Fabrics,TS195.6
  20. Studies on Impact of Transplanting the Flue-cured Tobacco Yield and Quality in Yongzhou,S572
  21. Antibacterial plastic with silver coating developed,TQ637

CLC: > Industrial Technology > Chemical Industry > Spices and cosmetics industries > Natural spices > Plant spices
© 2012 www.DissertationTopic.Net  Mobile