Dissertation > Excellent graduate degree dissertation topics show

Effects on Physiological Metabolism and Fermentation of Saccharomyces Cerevisiae with Proteinase A Deletion

Author: LiuXiaoJie
Tutor: ChenQiHe
School: Zhejiang University
Course: Food Engineering
Keywords: Brewer's yeast Protease gene Yeast protein Beer Fermentation Process Knockout Beer quality Yeast strains Beer Industry Foam stability Foam durability Yeast cells Master's degree thesis Wort Pyruvate kinase Crude protein content Cell disruption Medium Beer foam Tolerance test Fermentation late
CLC: X792
Type: Master's thesis
Year: 2012
Downloads: 11
Quote: 0
Read: Download Dissertation

Abstract


Proteinase A (prA) is one of the most significant aspartic proteinases in Sacharromyces cerevisaie. It plays an essential role in the protelytic maturation precesses of many vacuolar enzymes including many vacuolar proteinases such as preteinase B. In nitrogen starvation circunstances, proteinase A can hydrolyse both vacuolar and extracellular crude proteins so as to provide nitrogen source for new cells growth and sporulation. To beer industry, the residual prA in unpasteurized beer will hydrolyze those active-proteins in beer foam such as lipid transfer protein, and lead to a poorer foaming ability and worse foam ability, which will inversely influence beer quality. So in recent years, many researchers paid their attentions on the biological reconstruction of prA in order to extend beer shelf life. But if these changes can really meet the original expections and lead no negative influcene on the fermentitation abilities of the parent strain are still controversial. Based on this, we carried out this study. In this paper, the influences caused by the mission of prA on glucose and nitrogen metabolism, influences on circumstance stress tolerance ability, influences on fermentation abilities and influences on cell apotosis were studied. And results show that the mission of prA will obviously depress some glucolysis key enzymes activities and the accumulation of intracullar ATP. In nitrogen starvation circumstances, the activity of pyruvate kinase of the mutant strain was very low all over the first 4 days after inoculation, and the accumulation of intracellular crude protein in the mutant was much more than that of the parent strain. According to circumstamce stress, tolerance abilities of the mutant according to alcohol, H2O2 and low nitrogen stress was obviously dpressed. In beer fermentation, the fresh beer fermentated by the mutant was hard to flitration. The mission of prA lead to a higher apoptosis rate under circumstance stresses.

Related Dissertations

  1. Postprandial Lipids Levels after a Pure Fatty Meal and Their Relationships with Carotid Atherosclerosis in Type 2 Diabetics,R587.1
  2. On the Operating Mode of Relational Credit,F832.4
  3. Process-based empirical research enterprise technology innovation performance,F224
  4. Informal Finance and SME Financing Problem,F832.4
  5. Application of Rough Set and Flex in Mid-long Term Runoff Forecasting,P338
  6. Alleviate the Financial Dilemma of Small and Medium-sized Enterprises System Design,F276.3
  7. The Study and Development of Production Planning and Management System for Small and Medium Discrete Enterprises,TP311.52
  8. Isolation and Initial Identification of Human Ovarian Cancer Stem Cells,R737.31
  9. A Study of Perfecting the Legal System of Folk Financing by Small and Medium-sized Enterprise,F832.3
  10. Credit Strategy of Commercial Bank for Small and Medium-sized Enterprises,F832.4
  11. Establishment of a Staff Training System for SMEs,F276.3
  12. Human Resource Management in Small and Medium-sized Technology Enterprises Incentive Mechanism,F276.44
  13. Study on Employees’ Compensation Incentive Institution in Small and Medium-sized Private Enterprises,F272.92
  14. Research on the Degree of Satisfaction of the Staff in Medium and Small Private Enterprises,F272.92
  15. Insulin Resistance and Beta-cell Dysfunction in the Pathogenesis of Chinese Type 2 Diabetes Mellitus in Shenyang,R587.1
  16. The Processing Technology Studying of Fermented,TS251.65
  17. Effects and Influence on Glucose Metabolism of Replacement Therapy with Domestic Recombinant Human Growth Hormone in Children with Growth Hormone Deficiency,R725.8
  18. Screening of Serum Glucose and Clinical Study in Patients with Coronary Artery Disease.,R743.3
  19. Analysis of Clinical Presentations in a Large-Scale Chinese Women with Polycystic Ovary Syndrome (PCOS).,R711.75
  20. Study on the Third Oil Production Plant of Changqing Oilfield Company Medium-level Performance Management System,F272.92

CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > Light industrial waste disposal and comprehensive utilization > Food Industry
© 2012 www.DissertationTopic.Net  Mobile