Dissertation > Excellent graduate degree dissertation topics show

Functional Analysis of Solanum Torvum StPGIP Gene Related to Verticillium Resistance

Author: ZhuYanPing
Tutor: YangQing
School: Nanjing Agricultural College
Course: Cell Biology
Keywords: StPGIP potato tabacoo genetic transformation expression analysis disease resistance
CLC: S572
Type: Master's thesis
Year: 2011
Downloads: 10
Quote: 0
Read: Download Dissertation

Abstract


PGIP (Polygalacturonase-inhibiting protein) is an inhibitor to polygalacturonase secreted by pathogenic fungi. It mainly exists in the cell walls and endomembrane system and its content differs with growth time, varieties and organs. It has been reported that PGIP could suppress or delay the fungi growth by delaying the degrade process of pathogenic fungi cell wall.After the construction of the StPGIP over-expression vector, genetic transformation was carried out on potato and tobacco by Agrobacterium-mediated method. A total of 14 hygromycin B-positive tobacco lines and 16 hygromycin B-positive potato lines were obtained.5 tobacco lines and 6 potato lines were determined to be positive by PCR analysis. Southern blot analysis on 2 PCR positive tobacco lines and 4 PCR positive potato lines indicated that the StPGIP gene has been integrated into the genome of the potato and tobacco transgenic lines.Spatiotemporal expression analysis and induced expression analysis of StPGIP by RT-PCR and qRT-PCR revealed the different expression levels in different transgenic lines, and the expression level was higher in those plants with stronger disease resistance. Being compared with wild-type plants, the transgenic lines showed a higher expression level of StPGIP. Both of the transgenic and wild-type plants via the infection of Verticillium dahliae exhibited a higher level of StPGIP mRNA than the plants without infection. Additionally, the StPGIP expression of the transgenic and wild-type plants reached their highest expression point at the same time though there was a great difference in the later period, which can be confirmed by the research on the protein level of StPGIP of transgenic potato plants thereafter.The measurement of the soluble protein content by the SDS-PAGE electrophoresis and the antibacterial experiment in vitro were performed to analyze the StPGIP translation level. Before the infection of V. dahliae, the transgenic plants showed a higher protein level, compared with the wild-type plants. After the infection, the soluble protein content increased with different incensement in different time point. Moreover, the protein extraction from the transgenic plant showed an intensive inhibition on the growth of V. dahliae. The observation of phenotypes on the transgenic and wild-type plants after the infection of V. dahliae revealed that the transgenic plant exhibited a resistance to the V. dahliae infection within a certain time. At the same time, we found that the transgenic lines 11 was a high-resistant line.In conclusion, the overexpresion of StPGIP increased resistance to V. dahliae in transgenic tobacco as well as in transgenic potato, suggesting an evidence for application of StPGIP in plant Verticillium wilt resistance breeding.

Related Dissertations

  1. Study on Preparation of Composite Cross-linked Modified Modified Potato Starch,TS236.9
  2. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  3. Research on Rapid Freezing Process of French-fried Potatoes,TS215
  4. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  5. Incidence and Mechanisms of Insecticides Resistance to Pyrethroids and Endosulfan in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32
  6. Incidence and Mechanisms of Insecticide Resistances to Organophosphates and Carbamates in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32
  7. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  8. Molecular Clonging and Functional Analysis of Heat Shock Transcriptional Factor CarHSFB2 in Chickpea (Cicer Arietinum L.),Q943.2
  9. Studies on the Methodology for Resistance Identification of Rice Cultivar to Rice Black-Streaked Dwarf Disease and the Susceptible Growth Stages of Rice to Rbsdv,S435.111.4
  10. Toxicities of Several Newly-Typed Insecticides to Colorado Potato Beetle, Leptinotarsa Decemlineata (Say) and Molecular Cloning of Two Kinds of Targets,S435.32
  11. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  12. Influence of Controlled Partial Root Drying Irrigation on Water Use Efficiency of Potato,S532
  13. The Genetic Transformation and Functional Analysis of GmFtsH,S565.1
  14. Study on the Transgenic Tobacco Expressing Protein Kinase Like Gene TA50-10 of Wheat and Analysis of Disease Resistance,S572
  15. Cloning of Proteinase Inhibitor Gene CYS and API from Wild Potatoes and Preliminary Study of Potato and Tobacco Transformation with Spcys,S532
  16. Construction of Expression Vector and Genetic Transformation in Phalaenopsis of Phalaenopsis Floral Meristem Identity Gene LFY,S682.31
  17. Study on Transformation of Cut Chrysanthmemum with DdICE1 Gene,S682.11
  18. Study on Agrobacterium-Mediated Transformation of ’Benihonpe’ Strawberry with ALA Synthase Gene,S668.4
  19. Cloning and Expression of Responsive Genes Related to Cadmium Stress in Radish (Raphanus sativus L.),S631.1
  20. Preliminary Study on the Regeneration System and the Reporter Gene GFP Instantaneous Expression of Soft-seed Pomegranate Variety ’Tunisia’,S665.4

CLC: > Agricultural Sciences > Crop > Economic crops > Tobacco ( tobacco )
© 2012 www.DissertationTopic.Net  Mobile