Dissertation > Excellent graduate degree dissertation topics show

Near-Isogenic Line Development and Precise Mapping of Major Scab Resistance QTL Qfhi.nau-5A in Wheat

Author: TangMingZhi
Tutor: MaZhengQiang
School: Nanjing Agricultural College
Course: Biochemistry and Molecular Biology
Keywords: wheat Fusarium head blight QTL Wangshuibai Near-isogenic line Precise mapping
CLC: S512.1
Type: Master's thesis
Year: 2009
Downloads: 0
Quote: 0
Read: Download Dissertation

Abstract


Fusarium head blight or scab, caused mainly by Fusarium graminearum Schwabe, is a destructive disease of wheat (Triticum aestivum) worldwide, result in great yield losses and poor grain quality. Deployment of scab-resistant varieties is the most economical and effective strategy to curb this disease, and the genetic resistance is the basis of scab resistance breeding. As two of the best scab-resistant germplasms in the world, Wangshuibai and Sumai No.3 were researched much more on their genetic basis of scab resistance.Scab resistance of wheat is controlled by multiple genes and has been classified into two types:Resistance to initial infection (TypeⅠ) and Resistance to spread within the spike (TypeⅡ). We have identified two major QTLs against Fusarium graminearum infection in Wangshuibai by genetic mapping. In this study, near-isogenic lines (NILs) for one of the QTLs, namely Qfhi.nau-5A were developed using elite lines PH691 and Mianyang 2000-1 as the recurrent parents with the help of marker-assisted selection. Using PIS (percentage of infected spikes) as the typeⅠresistance index, resistance performance of the NILs were significantly high than recurrent parent. Genetic diversity analysis of Qfhi.nau-5A interval among six cultivars showed Wangshuibai and Sumai No.3 had the same genetic content in Qfhi.nau-5A region and the resistant cultivars very different from susceptible cultivars in the interval of Xbarc180-Xgwm304-Xgwm293 and Xmag3794 locus.For precise mapping Qjhi.nau-5A, recombinant plants of three genetic background were evaluated for FHB reaction. Using recombinants from the Nanda2419×Wangshuibai RIL population, recombinants with Mianyang 2000-1 background, and recombinants with Mianyang 99-323 background, Qfhi.nau-5A was placed in the interval of Xmag781～Xwmc446, Xbarc180-Xmag3794 and Xgwm415-Xgwm304. The three interval overlap each other, we precise mapped Qfhi.nau-5A within a genetic distance of 0.3 cM flanked by GWM415 and GWM304. Using Chinese Spring deletion lines, Qfhi.nau-5A linked molecular markers was physically mapped on 5AS chromosome C-5AS1-0.75 bin. The development of near-isogenic lines and precise mapping of the Qfhi.nau-5A could be used in disease resistance genes pyramiding and marker-assisted selection in wheat breeding program.

Related Dissertations

  1. Genetic Model Analysis and QTL Mapping of Agronomic and Quality Traits in Soybean,S565.1
  2. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  3. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  4. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  5. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  6. Genetic Analysis and Molecular Mapping of QTL for Rice Black-Streaked Dwarf Disease Resistance in Rice,S511
  7. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  8. Genetic Segregation Analysis and QTL Mapping as Well as Association Analysis for Panicle Angle Trait in Japonica Rice (Oryza Sativa L.),S511.22
  9. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  10. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  11. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  12. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  13. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  14. New Methodology for Mapping Resistance Trait Loci in Crop Cultivar Population,S336
  15. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  16. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  17. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  18. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  19. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  20. Differences in Winter Wheat Productivity and Nitrogen Use Efficiency between the Main Varieties Released in Different Times in Jianghuai Area,S512.1
  21. Quantifying the Responses of Weak Gluten Wheat Leaf Photosynthesis and Chlorophyll Fluorescence Parameters to Weak Light at Filling Stage,S512.1

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile