Dissertation > Excellent graduate degree dissertation topics show

Studies on Cytoplasmic Male Sterility and Restoration in Cotton (Gossypium Hirsutum L.)

Author: XuQi
Tutor: LiuKang
School: Nanjing Agricultural College
Course: Crop Genetics and Breeding
Keywords: Cotton Cytoplasmic male sterility (CMS) mitochondria cDNA-AFLP proteome
CLC: S562
Type: Master's thesis
Year: 2010
Downloads: 7
Quote: 0
Read: Download Dissertation


Plant cytoplasmic male sterility (CMS) is a maternally inherited trait that is unable to produce viable pollens, which plays an important role in utilization of hybrid vigor. As cotton cytoplasmic male sterility mechanism is unclear and the restore gene has not been cloned, the study of cotton CMS is far behind of maize and rice,etc.therefore,it has theoretical and practical significance for investigating the molecular mechanism of cotton CMS in order to utilize the cotton hybrid vigor better. A upland cotton CMS with the cytoplasm of G harknessii is controlled by cytoplasmic genes and repressed by nuclear restorer genes. Sterile-line anthers begin to degenerate in the sporogenous cell division stages and reach abortion peak in the pollen mother cell meiosis stage, so that it can not form tetrad, which is named as non-pollen male-sterility,and is appropriate for the hybrid seed production and study of the CMS mechanism.In this paper, the anthers of CMS, maintainer, restorer and Fi were employed to investigate the cotton CMS-associated and fertility restoring genes and proteins by using differential display of anthers cDNA-AFLP and mitochondrial proteome, the results were shown as follows: 1. Using sucrose Percoll and Nycdenze gradient ultracentrifugation technique to isolate anthers’ mitochondria from CMS, maintainer, restorer and F1. then identificate them by measuring mitochondrial marker enzyme and transmission electron microscopy,At last we establish a way to isolate highly qualified mitochondria from frozen cotton anthers.2. In the present research, the cDNA-AFLP technology was employed to analyze the differential gene expression profiles among the anthers of 6 days before anthesis of CMS line, maintainer line, restorer line, and F1, the results showed:that there is tremendous difference of the gene expression profiles of the anthers between the CMS line and its "isonuclear alloplasmic" maintainer line, suggesting that organelle gene may play important roles in modulation of the nuclear gene expression; the distorted nuclear genome expression triggered by CMS gene must contribute more directly to cotton cytoplasmic male sterility. Gene expression profile of the restored fertile plants of F1, i.e. the CMS line after introducing restorer gene, exhibited similar to that of the fertile lines including maintainer and restorer, and almost same as that of the restorer, implying that nuclear genes may counter the effect of CMS gene in regulating nuclear gene expression, thereby restore the fertility. The regulatory roles of CMS gene in modulating nuclear gene expression and the fact that the restoring gene may compromise this modulation effect must contribute a lot to explain the molecular mechanism of sterility and its restoration. Furthermore, we identified 36 amplified cDNA fragments which predominantly expressed in restorer line and F1, while showed no or very slight expression in CMS and maintainer lines, which observation shed lights on cloning of the restorer or restorer-related genes in cotton.3. By comparing mitochondrial proteome of CMS line with fertile lines and CMS line with restorer line, we obtain 46 CMS-associated proteins and 1 fertility restoring protein. Then, we identify 34 CMS-associated and 1 fertility restoring protein by MALDI-TOF/TOF.as cotton mitochondrial genome is hidden so, we only obtain 17 CMS-associated and 1 fertility restoring protein functional verification in the end.We divided the 34 CMS-associated proteins into five categories according to function, they were:energy metabolism related proteins, protein processing related protein, hormone synthesis related proteins, and toxin related proteins and other proteins. They confirmed that cotton CMS was related to energy metabolism, enzymatic activity, hormone and active oxygen.ATPase is an important enzyme in energy metabolism of living organisms. It is ATPase beta subunit related CMS fertility restoration. Therefore,We speculate that the ATP synthase beta subunit can restore the CMS mitochondrial energy metabolism By regulating the activity of the ATP synthase.Then restored the CMS fertility.

Related Dissertations

  1. Photosynthetic Apparatus Response to Heat Stress and the Mechanisms in Two Different Cotton with Various Leaf Colours,Q945.11
  2. Phosphoproteomics Studies on Cotton (Gossypium Hirsutum) Fiber Initiation Development,S562
  3. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  4. Control of Verticillium Wilt Disease of Cotton Plants with the Application of a Bio-Organic Fertilizer and Its Microbiologecal Mechanism in Rhizosphere,S144.1
  5. Yeast Two-Hybrid Screening of 14-3-3-Interacted Proteins During Early Cotton Fiber Development,S562
  6. Expressive Characterization of Transcription Factors in Cotton Fibre Initiation and Cloning and Characterization of Three Transcription Factor Genes in Gossypium Hirsutum L.,Q943
  7. Selection and Optimization of Control Technics in CODCr and Colority of the Refined Cotton Wastewater Treatment Process in a Refined Cotton Enterprise in Rudong, Jiangsu Province,X791
  8. Determination of Soil Enzyme Activity and Extraction, Purification and MS Identification of Soil Microbial Total Protein,S154
  9. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  10. Fitness Costs of a Disrupted Cadherin Allele Conferring Cry1Ac Resistance of Helicoverpa Armigera,S435.622.3
  11. Preparition and Biological Control Effect of Microbial Organical Fertilizer Against Cotton Verticillium Wilt,S435.621
  12. Study on the Recovery of Growth and Its Physiological Mechanisim of Cotton After Waterlogging During the Bud Stage and Flowering and Boll-Forming Stage,S562
  13. Effects of Shading on Growth and Nitrogen Metabolism in Cotton Plant (Gossypium Hirsutum L.) during Flowering and Boll-Forming Stage,S562
  14. Effects of Salinity Stress on Cotton (Gossypium Hirsutum L.) Root Growth and Cotton Field Soil Micro-Ecology,S562
  15. Effect on Yield and Quality of Cotton with Soil Waterloged and the Physiological Basis,S562
  16. Study on the Morphology, Physiology and QTLS Mapping of a Dwarf Mutant Ari1327 from Cotton (Gossypium Hirsutum L.),S562
  17. Preliminary Function Analysis of Two Annexins in Cotton (Gossypium Hirsutum L.),S562
  18. SSR Marker-Assisted Pyramiding of Verticillum Wilt Resistant QTL in Upland Cotton,S562
  19. The cDNA-AFLP Analysis of Gene Differential Expression and RNA Editing of ATP6 Gene between Cytoplasmic Male Sterile Lines and Their Maintainer Lines in Soybean (Glycine max (L.) Merrill),S565.1
  20. The Genes Expression Analysis of Cotton Fiber Initiation Stage and Characterization of Three New Genes in Gossypium,S562
  21. The Molecular Evolution Analysis of Genes Related with Cotton Fiber Development in Different Cotton Species in Gossypium,S562

CLC: > Agricultural Sciences > Crop > Economic crops > Fiber crops > Cotton
© 2012 www.DissertationTopic.Net  Mobile