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Study on Propagation Culture of LAB and Processing of DVS Yogurt Starters

Author: LiangMinShan
Tutor: JiangQiBin
School: West China University
Course: Of Food Science
Keywords: Lactobacillus plantarum Proliferation culture Lyophilized protection DVS yoghurt culture
CLC: TS252.4
Type: Master's thesis
Year: 2010
Downloads: 209
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Abstract


Fermented dairy products with the development of the dairy industry, with its rich nutrition and pleasant taste, more and more consumers and researchers concerned. Dairy researchers in the field have mainly focused on DVS yoghurt culture, which is of great significance for improving the competitiveness of domestic fermentation agent, and to promote the development of the dairy industry. The test mainly discuss the proliferation of Lactobacillus plantarum culture conditions, bacterial concentration and separation processes, screening and optimization of lactic acid bacteria foundation enrichment medium and Lyoprotectant of quality and efficient DVS yoghurt culture prepared. The powder will later access through the activation of four different medium, and ultimately selected proliferative effect preferred MRS medium as a basal medium for use by the front of the pilot phase and to lay the foundation for the next step of the concentrated culture. In order to understand the growth of Lactobacillus plantarum and grasp the regularity of its growth, the culture liquid of the Lactobacillus 30h tracking measurement according to the pH value, 0D value and the number of viable cells were drawn and the corresponding growth curves. Timely adjust the acidity of the culture medium by adding buffers to inhibit Lactobacillus plantarum 'autolysis', to create a good environment for their growth and reproduction, and thus effectively reduce mortality. The results show that the addition of 2% buffer Ⅰ can play a very good stabilizing effect. The growth curve of lactic acid bacteria, some changes occurred adding buffers need to be further determined by measuring its variation. \That: after adding buffers lactic acid bacteria viable count maximum time from 15h to 18h, the lactic acid bacteria 3h extended logarithmic phase. Appropriate growth factor and a better foundation medium formula is determined by a single factor and orthogonal experiment results: sucrose, yeast extract and potato juice is more to promote the growth of Lactobacillus plantarum, an increase of 2% sucrose in the basal medium, 2% yeast extract and 10% potato juice cultivate the 6.88 × 1010cfu/ml after the number of viable cells from increase to 1.58 × 1011cfu/ml. Concentration and separation stage separation enrichment by centrifugation, drawn and tested: 0 ° C, 3000rpm, 10min of centrifugation conditions can be more reasonable for lactic acid bacteria bacterial sludge and the number of viable cells, also good to consider the cost problem. Product features and the number of viable cells, and finally select the freeze-drying method as the last drying method of using spray-drying and freeze-dried two drying method. Lactobacillus added after concentrated by centrifugation in which the lyoprotectant to prevent lactic acid bacteria of the large number of deaths in the freezing process. Using glycerol, Tween 80 and sodium glutamate lyophilized protection play better after adding the role of the protection of bacteria, and the added amount: Tween 80 0.5 ml of / L, glycerol 0.5 ml of / L, glutamyl sodium 5g / L, cultured the solution lyophilized viable count reaches 2.69 × 1011cfu/ml.

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