Dissertation > Excellent graduate degree dissertation topics show

Heme Oxygenase is Involved in Salinity-Induced Tomato Lateral Root Development and Its Relationship with Hydrogen Peroxide

Author: ShiZuoZuo
Tutor: ShenWenZuo
School: Nanjing Agricultural College
Course: Biochemistry and Molecular Biology
Keywords: NaCl Heme oxygenase Cell cycle gene Hydrogen peroxide Tomato lateral root
CLC: S641.2
Type: Master's thesis
Year: 2009
Downloads: 8
Quote: 0
Read: Download Dissertation


Heme oxygenese has recently been proved to be an important signal molecule in mammalian cells, and plays a putative role in keeping cell survival and stabilization. Recently, some results proved that HO was involved in modulating the tolerance of salt stress, inducing stomata closure and promoting adventitious roots development.To elucidate the function of HO in the development of lateral toot induced by salinity, we identify the effects of endogenous HO induced by NaCl on lateral root formation and its signal transduction. In this report, we discovered that NaCl induced lateral root development in a dose-dependent manner, also increased LeHO-1/2 transcripts and HO-1 protein expression. In order to check whether HO is involved in the lateral rooting process, HO-1 specific inhibitor ZnPP and HO-1 inducer hemin was applied. The application of ZnPP could reverse the inducing effects of 5 mM NaCl, and hemin could recover the inhibition effects of 25 mM NaCl on lateral root development and HO activity. The molecular mechanism further indicates that NaCl promotes the development of lateral root by up-regulating cell cycle gene LeCYCD3;1, LeCDKAl, LeCYCA2;1 and LeCYCA3;1, and down-regulating LeKRP2 transcripts, while ZnPP reverses the effects. Thus, above results indicate that HO was involved in NaCl-induced lateral root development by modulating cell cycle gene expression.Moreover, we investigated the role of H2O2 in tomato lateral rooting process. The fluorescence detection results found that salt-induced H2O2 could be scavenged by the H2O2 scavenger CAT and DMTU, leading to the reduction of lateral root formation. CAT and DMTU could also inhibit HO transcripts, HO-1 protein expression and HO activity. Exogenous H2O2 treatment could mimic the effects of endogenous H2O2 on the formation of tomato lateral root and HO expression. It was also found that H2O2 promotes the development of lateral root by up-regulating cell cycle gene LeCYCD3;1 and LeCDKAl and down-regulating LeKRP2 transcripts, while ZnPP reverses these effects. These suggest that H2O2 may act as the upstream signal molecule of HO action.Taken together, we provided pharmacological, physiological and molecular evidence that the signal transduction in lateral root development is mediated by NaCl→H2O2→HO→LR development.

Related Dissertations

  1. Study of Inhibitors of Aluminum Anode in NaCl Solution,TG174.42
  2. Studies under Ultra-high Pressure on Goose,TS251.1
  3. New Technology and Basic Study on the Direet Preparation of PbO Powder from Galena by Selective Leaching Technology,TQ134.33
  4. Heme Oxygenase is Involved in Cytokinin-Induced Alleviation of Senescence Induced by Darkness in Detached Wheat Leaves,S512.1
  5. The Effects of Exogenous H2O2 and AsA on Hyperhydricity and the Characteristics of Endogenous Reactive Oxygen Species Metabolism of the Garlic Plantlet in Vitro,S633.4
  6. Heme Oxygenase-1 is Involved in the Modulatory Role of Salicylic Acid in Cadmium-Induced Oxidative Stress in Medicago Sativa,S541.9
  7. The Modulatory Role and Relationship between Heme Oxygenase and Reduced Glutathione in High-Iron-Induced Oxidative Stress in Detached Leaves of Oryza Sativa L.,S511
  8. Kinase Activities Analysis and Localization of ZmCCaMK Induced by Abscisic Acid in Leaves of Maize Plants,S513
  9. Cross-Talk between Ethylene and Heme-Heme Oxygenase Signal System in Primary Root Growth Inhibition of Etiolated Tomato Seedlings,S641.2
  10. The Involvement of Heme Oxygenase/Carbon Monoxide System in Cobalt Chloride-Induced Lateral Root Development in Tomato Seedlings,S641.2
  11. Responses of Wheat and Tomato Heme Oxygenase to Hydrogen Peroxide and Heat Shock Stresses and its Role in Antioxidative Defense,S512.1
  12. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  13. The Effects of Reserving Time of Filling Urokinase on Stereotactic Hematoma Aspiration of the Rabbit Intracerebral Hemotoma and the Heme Oxygenase -1 (HO-1) Expression,R743.3
  14. The Research on Hydrogen Peroxide Sensor Based on Nanomaterials Modified Electrodes,TP212.2
  15. The Effect of Different Concentration of Urokinase on Stereotactic Aspiration of the Rabbit Intracerebral Hematoma and the Expression of the Heme Oxygenase-1,R743.34
  16. Effect of Hydrogen Peroxide and X-ray on Calcium Oscillation in Airway Epithelial Cells and Pulmonary Macrophages,R563.9
  17. Study on the Different Expression of Heme Oxygenase Enzyme in Human Testis of Fertile and Infertile Males,R698.2
  18. Carbide slurry sulfide and cyanide treatment process,X701.3
  19. Cotton fabrics cryogenic hydrogen peroxide bleaching system research,TS192
  20. Five kinds of papaya salt tolerance of plants,S685.99

CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Solanaceous > Tomatoes ( tomatoes )
© 2012 www.DissertationTopic.Net  Mobile