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Lignocellulose is the most abundant on earth, the cheapest, transformed by microbial fermentation of sugars resources for the renewable energy degradation of various types of cellulose to obtain a variety of energy has become a highly topical issue around the world. China is a large agricultural biomass resources are very rich, but the low energy density of biomass resources in China, widely distributed, is not easy to collect use. Cellulose conversion technology research and development straw based raw materials, the development of new energy sources, environmental protection has a very important practical significance. Straw substances from the perspective of sustainable development, as a raw material for hydrogen production will be the hydrogen production industry is a very promising development direction. The paper is a \Based on crop stalks own ingredients (lignocellulose pretreatment into renewable sugars resources) characteristics and photosynthetic bacteria to produce hydrogen research group screened photosynthetic bacteria mixed flora, research in hydrogen production by photosynthetic bacteria process as the best pretreatment conditions for hydrogen production from raw material to the straw substances, and further study of the system of biological hydrogen system in optimal pretreatment conditions the amount of hydrogen production and hydrogen production rate, straw hydrogen production ability, the purpose is to provide reference and basis for the study of photosynthetic hydrogen production crop straw. The results show that: (1) found that these factors through the study of the conditions of pH, pretreatment temperature, raw material particle size single factor reducing sugar yield of crop stalks of acetic acid pretreatment has a more significant impact. Acetic acid pretreatment crop straw pretreatment conditions were obtained by a single factor: raw material particle size to the ultrafine (300nm), pretreatment temperature of 121 ° C, the acetic acid concentration of 25%, the ratio of 1:20 pretreatment time of 30 min, pretreated feed solution adjusted to pH 7.0, the highest rate of reducing sugar. (2) identify the primary and secondary relationship of the various factors that impact on reducing sugar yield from the orthogonal experimental design and experimental results of the single factor: the granularity → pretreatment temperature → pretreatment time → → acetic acid concentration ratio of solid . By the variance analysis, the raw material particle size, the concentration of acetic acid, the pretreatment temperature of acetic acid pretreatment sorghum reducing sugar yield more significantly, wherein the influence of the raw material particle size is the most significant influence factors. By orthogonal verify experimental results shows that the best of acetic acid pretreatment process conditions for the crop straw: the particle size is selected as Advanced Micro Devices, the acetic acid concentration of 25%, the ratio of 1:15, the pretreatment temperature of 121 ° C, pre-processing time for 20min. (3) by changing the substrate concentration, temperature, light intensity, inoculum size, the use of a pH of 7.0, inoculum culture 48h OD value of 0.4 to 0.6 in the logarithmic phase of photosynthetic bacteria, after acetic acid pretreatment of straw matrix for hydrogen production, the amount of hydrogen production and hydrogen production rate under different conditions to understand the use of acetic acid pretreatment optimal hydrogen production condition under the conditions of crop straw by the results: should choose straw substrate concentration of 60 g / L, the initial temperature of 30 ℃, light intensity 3000Lx, inoculum size 30%.
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