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Chlamys farreri (Chlamys farreri ) collected the papers from a farming area in Weihai, Shandong ontogeny in embryos and larvae of the period , including the blastocyst stage , gastrula , trochophore larval stage , early D-shaped larvae , D -shaped larval stage , shell top and spat . Samples collected from the C. farreri blood cell monoclonal antibody library fixed , paraffin-embedded sections , histochemical larval morphological changes observed in each period ; screened a fluorescent positive strong , and other tissue cells without cross the monoclonal antibody , using it as a tracer of blood cells , using immunohistochemical method to observe and analyze samples of Chlamys farreri blood cell , occur with positioning . The results showed that the positive signal for the first time in about two days after fertilization, D-shaped larval stage , the distribution of positive blood cells in the mantle, the base of the face plate of the larvae , digestive tract , digestive gland , foot and other tissues and organs . Discernible positive blood cells appear in the top housing of 7 days after fertilization , distributed in the larvae of the mantle surface of the tray base , the digestive gland and gastrointestinal and around , the cells were oval , approximately 3 to 5 μm diameter . Spats period of about 15 days after fertilization , extragonadal , development of various tissues and organs of the juveniles and Tony almost the same , can be clearly observed particles of blood cells in the mantle, gills , digestive gland , the digestive tract , and adductor muscle around distribution, the cells were oval, approximately from 4 ~~ 6 μm diameter . Concluded that , of C. farreri the granulosa cells appear in the early development of the D -shaped larval stage , and distribution of blood cells in the ingestion , digestion , respiratory -related , such as the face plate , mantle and digestive gland and gastrointestinal tissues and organs in and around .
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