Dissertation > Excellent graduate degree dissertation topics show

Mechnism of Pathogenesis Mediated by Key Amino Acid of Cucumber Mosaic Virus 2b Protein

Author: LiXiYing
Tutor: ChenJiShuang
School: Zhejiang University of Technology
Course: Biochemistry and Molecular Biology
Keywords: Cucumber mosaic virus 2b protein Post-transcriptional gene silencing AGO RDR
CLC: S432.41
Type: Master's thesis
Year: 2010
Downloads: 67
Quote: 0
Read: Download Dissertation

Abstract


Cucumber mosaic virus (Cucumber Mosaic Virus, CMV) is one of the most serious harm to the agricultural economy of the plant virus. CMV 2b protein plays a causative factor in the pathogenesis of CMV RNA silencing promoters and virus long-distance move decision factors and other important role. Therefore, to clarify the pathogenesis of CMV 2b protein has important theoretical significance for the development of to control CMV new methods and new strategy. 2b gene recombinant virus FRad35 2b -CMV Rad35-CMV 2b gene strains accurate replacement Fny-CMV strain is built in a variety of tobacco host, the recombinant virus induced light flower leaf symptoms. Mutant FRad35 2bLeu -CMV virus and FRad35 2b -CMV just one amino acid difference, i.e. # 55 Leu by FRad35 2b -CMV Pro mutation from. FRad35 2bLeu -CMV in N. benthamiana caused serious curled leaves, plant height, necrosis and even plants. Northern blot analysis -CMV and FRad35 2bLeu -CMV in N. benthamiana host progeny RNA accumulation FRad35 2b, 14 days after inoculation, the results show: FRad35 < sup> 2bLeu -CMV progeny RNA accumulation was significantly higher than FRad35 2b -CMV. Thus, FRad35 2bLeu -CMV virulent their progeny RNA accumulation in the host body. Has the highly pathogenic FRad35 2bLeu -CMV significantly inhibit host N. benthamiana AGO family by quantitative PCR gene silencing pathway in protein the factor-ago family of proteins and RDR protein, the results show: AGO1-1, AGO4-1 expression, while the weakly pathogenic FRad35 2b -CMV infection in N. benthamiana, these silencers factor expression and Mock compared to control both liter high. Based on previous studies, the use of constructed containing FRad35 2b -CMV and FRad35 2b Leu-CMV 2b gene of Agrobacterium transient expression vector 35S-2B and 35S-2b Leu 2b protein transient expression of exogenous GFP post-transcriptional gene silencing. Electricity into Agrobacterium tumefaciens containing different carriers and express the 2b protein Agrobacterium different with 35S-GFP Agrobacterium co-infiltration, transient expression of GFP and 2b protein in turn GFP N. benthamiana 16c leaf tissue. Co-infiltration on day 3, 35S-2b Leu and 35S-Fny2b of infiltration area appears bright green spots, 35S-2b Pro infiltration darken, GFP mRNA Northern blot analysis to determine the massive accumulation of GFP mRNA in the 35S-2b Leu and 35S-Fny2b of infiltration area; seven days after co-infiltration, despite the 35S-Fny2b and 35S-2b Leu still show bright fluorescence signal, but both infiltration area GFPmRNA accumulation was significantly lower than mock control, and 35S-2b Pro , and these results illustrate of 2b Leu Although the initial partial silencing of GFP was significantly inhibited, but over time, 2b Leu does not inhibit the infiltration area siRNA generated, resulting in localized GFP mRNA degradation. The 14th day of the co-infiltration, infiltration of 35S-2b Pro the top of the plant leaves performance of GFP system silence, and the infiltration of 35S-2b Leu plants remained significant green its top leaf GFP is not induced silencing. Northern blot results also show that: in the top of the plant leaves infiltration 35S-2b Pro detected a small amount of GFP mRNA accumulation was significantly lower than in the 35S-2b Leu or 35S accumulation in the top of the plants-Fny2b infiltration system leaves. The results show: compared with Mock, a bright green spots 35S-2b Leu infiltration AGO4 gene silencing pathway by quantitative PCR three days infiltration area of ??AGO4-1 and RDR6 -1 and RDR6 accumulation, subject to inhibition, the GFP degradation 35S-2b Pro infiltration area AGO4-1 and the expression amount of RDR6 but elevated to a certain extent. Therefore, we hypothesized that 55 Leu have a certain impact on the high-level structure of the 2b protein, which play an inhibitory effect on of AGO4-1 and RDR6, so as to achieve the purpose of suppression of systemic silencing and DNA methylation. In summary, we hypothesized that the highly pathogenic 2b Leu -mediated prevent siRNA system through its mobile, and inhibit the expression of the AGO4-1 and RDR6 silence factor to achieve efficient virus system to move and copy, resulting host showed severe symptoms of the virus.

Related Dissertations

  1. Patients with panic disorder attention automatic processing auditory information before the event-related potential study,R749.7
  2. Shandong Province 64kg men's boxers before Zhiquan Biomechanical analysis technique,G886.1
  3. Study of Transgenic Technology in Processing Tomato Resist Cucumber Mosaic Virus,Q943.2
  4. The Molecular Detection of Important Viruses and Population Dynamics on Processing Tomato in North Xinjiang,S436.412.1
  5. Before the Tang Dynasty Fu Sports,I207.22
  6. Construction and Genetic Transformation of Artificial MicroRNAs for Interfering Cucumber Mosaic Virus (CMV),Q78
  7. Watercolor watercolor ago,J215
  8. Tang Romances in front of male images,I207.41
  9. The Preliminary Research of Processing Tomato Interaction with ToMV and CMV Relatives Proteins,S436.412
  10. Effect of Neonatal Maternal Separations on Visceral Hyperalgesia in the Developing Rats and FOS Expression in Hypothalamus Paraventricular Nucleus and Anterior Cingulated Cortex,R748
  11. Low-dose heparin before intervention DIC in sepsis prospective clinical study,R459.7
  12. Task-based teaching model in high school English reading teaching,G633.41
  13. Studies on the Clone of Pepper Resistance Gene Analogues and Molecular Marker Linked to Cucumber Mosaic Virus (CMV),S641.3
  14. Gene Expression Analysis of Psuedo-recombination between Cucumber Mosaic Virus M and Fny Strains in Systemically Infected Nicotiana Benthamiana Seedlings,S432.41
  15. Al / AgO battery cathode materials Preparation and Properties,TM912
  16. The Design of Acoustic Logging System Based on 8 Channels,TP274
  17. Tomato antiviral gene cloning and comparative analysis,S641.2
  18. The Complete Genomic Sequence and Infectious Clones of Cucumber Mosaic Virus Infecting Musa Basjoo,S436.8
  19. Gene Diversity of Cucumber Mosaic Virus Infecting Field Tomato in Eastern China,S436.412.1
  20. Before the watershed landscape pattern of yellow and dynamics of,S181

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant diseases and their prevention > Transgression ( pass ) an infectious agent harmful > Virus
© 2012 www.DissertationTopic.Net  Mobile