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Recently, the disease rate of the metabolic syndrome with the hyperuricemic and insulin resistance is increasing. The metabolic syndrome is caused by the accumulation of a group of diseases with insulin resistance as the central, whose typical signs are high blood pressure, obesity, dyslipidemia, and hyperglycemia and so on. Additionally, the insulin resistance also accouts for the increased level of the uric acid, and is a dangerous facor for the hyperuricemia. As the level of the uric acid is increasing beyond the standard, the massive accumulation of the urate will lead to the cardiovascular diseases and intensify the insulin resistance. And the relationship between these two has become the heated research both at home and abroad. It is payed widespread attention to by controlling and improving the patients’ condition through dietary factors.Objective:To study the effect of the casein phosphopeptides combined with high calcium diet on the hyperuricemia model and the insulin resistance combined with hyperuricemia model animals and the available mechanisms through observing the changes of xanthine oxidase, adenosine deaminase and other enzymes related with uric acid metabolism during this process.Materials and methods:1. Ten rats were picked up from 50 as controlled randomly. The others were gavaged by adenine and ethambutol for three weeks to make up a hyperuricemia rat model successfully, and divided into four groups, Ca, CPPs, the compound, and model sets. The four groups of hyperuricemia rats were fed by feeding stuff with CaHPO4, with CPPs, with the compound and normal respectively for 28 days. Many indexes were texted, including uric acid, total cholesterol, triglycercide, glucose in the serum, and superoxide dismutase, malondialdehyde, and catalase in the serum and liver, together with the activity changes of the rats xanthine oxidase, adenosine deaminase and guaninedeaminase in the serum and liver.2. The same methods as it in part 1 was utilized to make up a hyperuricemia rat model successfully, and the rats was divided into four groups, low, middle, high, and model sets. The four groups of hyperuricemia rats were fed by feeding stuff with different dosages compounds and normal respectively for 28 days, and then the same indexes as it in part 1 were texted.3. Ten rats were picked up from 50 as controlled at random. The others were fed by high-fat, high-sugar and high-salt for eight weeks and then gavaged by adenine and ethambutol for three weeks to make up an insulin resistance model combined with hyperuricemia rat model successfully. The 40 rats were divided into four groups, low, middle, high, and model sets. The four groups of hyperuricemia rats were fed by feeding stuff with different dosages compounds and normal respectively for 28 days, and then the same indexes as it in part 1 were texted.Results:1. The uric acid, total cholesterol, triglycercide, malondialdehyde levels and activity of adenosine deaminase in the liver of the rats with intervention by the compound were lower than those of model group rats, significantly (P<0.05).2. The uric acid and liver malondialdehyde levels in the all dosage groups of rats were lower than the model group rats. The difference between high and low dosage groups was significant (P＜0.05). The activities of adenosine deaminase both in serum and liver of all dosage groups of rats were lower than those of model ones (P＜0.05).3. The uric acid, total cholesterol, triglycercide, glucose and insulin levels and HOMA-IR in the all dosage groups of rats were lower than the model group rats. The effect was enhanced with the increasing dosage.Conclusions:The casein phosphopetides combined with the high calcium had the relatively obvious effect on decreasing serum uric acid compared with other intervention methods. This effect was strengthened as increasing dosage of the compound. Based on the fact, the mechanism was speculated that the intervering substances could descrease the activity of xanthine oxidase in the liver, and adenosine deaminase in serum and liver. Meanwhile, casein phosphopeptides was playing a role in promoting absorption and using of calcium from the diet that was important to decrease the serum total triglyceride, total cholesterol and increase the insulin sensitivity. The decreased levels of serum insulin and blood glucose could accelerate the serum uric acid reduction.
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