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Dilophodes elegans sinica Prout was the main defoliator in Illicium verum. The development of Illicium verum industry was seriously hindered, and the major economic was immeasurably lost when this insect attack severely occurring. In order to realize the effective management and make use of the pests, and improve value-added of Illicium verum industry, the author preliminary studied on the biological activity of Dilophodes elegans sinica Prout frass and its host plant extracts. The results were summarized as follows:1. The main volatile matters of Dilophodes elegans sinica Prout frass were detected by means of Solid Phase microextraction and Gas Chromatography-Mass Spectrometry (GC-MS), they were anisole p-propenyl(83.24%),α-farnesene, (2.95%), bicycle[3.1.1]hept-2-ene,2,6-dimethyl-6-(4-methyl-3-pentenyl)(4.20%), undecanoic acid, methyl ester (0.71%), nonadecanoic acid, ethyl ester(1.37%),11-octadecenoic acid methyl ester(2.40%) respectively. The six main volatile matters occupied 94.87% of the total in the peak area.2. Dilophodes elegans sinica Prout adult showed the strong EAG responses to the volatile matters of Dilophodes elegans sinica Prout frass. Among the five compounds tested, EAG responses of Dilophodes elegans sinica Prout adult to Anisole, p-propenyl were stronger than that of other compounds tested, but the weakest to nonadecanoic acid, ethyl ester, all of the compounds no representation sex difference except anisole, p-propenyl. Dosage response curves of EAG of Dilophodes elegans sinica Prout adult showed that the male and the female adult showed positive correlation EAG response to concentration’s varying of undecanoic acid, methyl ester and anisole, p-propenyl and 11-octadecenoic acid, methyl ester, showed "U" model EAG response to the a-farnesene, showed the maximum value of EAG response to nonadecanoic acid, ethyl ester in 0.01 (v/v). The EAG responses of female and male adults to different compositions mixture showed that there were no synergy effects between anisole, p-propenyl and other compositions to female. But the males showed synergy effects between anisole, p-propenyl and a-farnesene, undecanoic acid, methyl ester.3. The bacteriostasis effect of different extracts of larvae frass to different strains existed some difference. Acetone extracts had a stronger effect to bacterium which including S. aureus and E. coli, and distilled water extracts had a stronger effect to fungus which including P. curinum and R. stolonifer.4. All of the different extracts from the larvae frass in this paper had scavenging activity to the free radicals including DPPH free radical,-OH free radical,and O2- free radical. Concretely speaking, the extracts from the larvae frass had a weak scavenging activity to DPPH free radical, which was significantly weaker than the extract from Illicium verum leaves under the same concentration. The extracts of chloroform and ethyl acetate had a much stronger scavenging activity to the·OH free radical, while the scavenging activity of extracts of Petroleum ether, butanol and distilled water to·OH free radical was weaker than that of extracts from Illicium verum leaves. The scavenging activity from larvae frass to the O2- free radical was weaker than that of extracts from I. verum leaves under the same conditions.5. The ethanol, acetone, ethyl acetate and petroleum ether, etc. four extracts all had a strong repellent and fumigate effect, and the repellent and fumigate effect of the acetone extract was strongest while that of petroleum ether extract was weakest among them. In various extracts of acetone extract, the petroleum ether extracts had the best fumigate action, which included 21 kinds of chemical composition of olefine, alcohols, acids, ketones and so on, and the most content were p-anisaldehyde and anethole among all kinds of chemical composition.6. The ethanol and acetone extracts of I, verum leaves showed a stimulative effect to three protective enzymes (CAI、POD and SOD) of adults of G. ficorum in the early treatment and a inhibition effect in the late treatment. The carboxylesterase activity in adults of G. ficorum significantly increased when the two solvent extracts treated on G. ficorum after two hours. The CAT activity of the treated G. ficorum rapidly decreased down to lower than untreated group when the acetone extract treated after six hours. On the contrary, the CAT activity kept to rise during a long time to maintain a high level when the ethanol extract treated after six hours. The two solvent extracts above showed a stimulative effect to GSTs six hours before treatment and showed a inhibition effect six hours after treatment.
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