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With China's sustained, rapid development, growing lawn area from the courtyard, landscaping to the playground, water conservation, sand control, saline-alkali soil improvement and other areas. Accelerated urbanization lawn industry has brought to China's rapid development opportunities. But more than 90% of turfgrass is to rely on foreign imports. These introduced species in biology as well as local non-adaptive With the introduction of ecological problems caused by the expansion of the more obvious each day, the development and utilization of domestic high-quality turfgrass is our turf industry problems to be solved. Our complex and diverse climate, topography, soil and other environmental cause of wild grass lawn with rich resources and genetic variability of ecological types, the development prospects of large, has attracted the attention of domestic breeding expert. In order to better exploitation abundance of wild turfgrass germplasm resources, the study used AFLP markers on bermudagrass and seashore paspalum germplasm material identification and genetic diversity were studied. In this experiment, collected 36 wild species and cultivars of bermudagrass for the study of materials, from morphology and DNA molecular level for the test materials to study the genetic diversity, the purpose of the genetic material of proven provenance relations to establishing fingerprint for bermudagrass effective protection of wild germplasm resources, development, utilization and construction of core collection to provide theoretical and practical scientific data. The results showed that: (1) 16 pairs of band pattern stable, clear uniform polymorphic primer pairs were amplified polymorphic markers 327 polymorphic loci ratio was 81.75%, among the tested materials There are obvious differences; (2) primer combinations E47M48, E33M48, E47M63, E51M66, E47M59 specifically constructed DNA fingerprinting was tested materials can be distinguished from each other; (3) for the test material AFLP similarity coefficient 0.5286- 0.8857 between. The results from the molecular cluster analysis can be seen, all materials in similarity coefficient 0.590 clustered into one large group, including 31 from the three provinces of wild bermudagrass materials in addition to 6,7,21 Number Material outside the similarity coefficient of 0.698 clustered into one large group, showing a close genetic relationship; (4) the morphological characteristics of the tested materials genetic distance (TD) in between 0.483-7.960. The results showed that the genetic distance between 9.21-10.95, tested materials can be grouped into three categories. Which were from Fujian Province, a total of 10 wild bermudagrass materials, in addition to material outside No. 13,16,19, genetic distance of 4.01 in Jinhua, Zhejiang No. 1 from poly material for the class Ⅰ. To solve seashore paspalum (Paspalum vaginatum) propagation process large variety purity nutritional problems, this study AFLP marker technology, combined with morphological characteristics of seashore paspalum cultivars 'Salam', 'SeaSpray', 'SeaDwarf', 'SeaIsle 2000' and a 'SeaDwarf' thick stems Mutant genetic differences were analyzed to investigate the seashore paspalum cultivars rapid identification methods. The results showed that: (1) seashore paspalum genetic distance and morphological similarity coefficient AFLP markers consistent clustering results, show that 'Salam' and 'SeaSpray' genetic differences between small and can be clustered together, ' SeaDwarf 'and' SeaIsle2000 'close genetic relationship can be clustered in another; (2)' SeaDwarf 'thick stems mutant lines with' SeaDwarf 'AFLP markers between genetic similarity coefficient was 0.5281, morphology, genetic distance is 4.8570, indicate that the genetic difference between the two, in terms of morphology, or AFLP markers were confirmed 'SeaDwarf' thick stems indeed has mutated strains; (3) there are 11 pairs of AFLP primer random combination with type of stable, clear uniform, polymorphism good, seashore paspalum cultivars by AFLP fingerprinting obvious differences; (4) found a distinction seashore paspalum cultivars 8 DNA characteristic bands, further illustrate the method of AFLP markers There may be used to seashore paspalum cultivars or mutants assisted identification.
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