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Extraction of Soybean Protein Isolate by Multi-stage Countercurrent Solid-liquid Extraction

Author: GaoJianPing
Tutor: WangMingLin
School: Shandong Agricultural University
Course: Of Food Science
Keywords: Soy protein isolate Liquid chromatography MS A multistage countercurrent solid-liquid extraction Release behavior
CLC: TQ936.2
Type: Master's thesis
Year: 2011
Downloads: 99
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Abstract


The soy protein isolate (SPI) in defatted soybean meal prepared from raw materials through alkali-soluble and acid precipitation of protein products, traditional extraction process to extract low water consumption, whey wastewater emissions. Soybean meal protein, types of mass spectrometry-based protein identification based on the effects of extraction conditions on the release behavior of different types of protein soybean meal, and the establishment of the SPI-based the multistage countercurrent solid-liquid extraction techniques to extract new technology, Based on this study and the establishment of the soybean processing industry whey protein extraction of high value-added and comprehensive utilization, aimed at reducing the water consumption of the extraction process and improve protein yield. The main conclusions are as follows: 1. HPLC separation of protein extracts and whey collected isolated main protein component; enzyme technology combined with liquid chromatography-mass spectrometry protein isolated species identification. The results show that a wide range of the retention time distribution of the different kinds of proteins, the retention time in the chromatogram of the protein extract of 7S and 11S components are mainly concentrated in the subsequent 24 min, containing a small amount of 2S component, whey main 2S component, its retention time was 24 min ago. The time of flight mass spectrometry, and gel filtration chromatography analysis of protein extracts and whey; results showed that the molecular weight range of the SPI 10 kDa-320 kDa, the molecular weight range of the whey 10 - kDa-30 kDa. Protein components identified on the basis of the protein in soybean meal dissolution process monitoring, research and SPI extract the release behavior of soybean meal protein components in the process. The results show that the faster release rate 2S component After three extraction capacity of 84% relative emission can reach more than 90%; 7S and 11S component release rate than 2S low release rates of the components, the four extracted relative release , increase the concentration gradient between the solid and liquid phases in the extraction process and the extension of the 7S and 11S fractions the relative extraction time is conducive to improve the release rate of the 7S and 11S fractions. 3 examines the impact of the multi-stage countercurrent solid-liquid extraction process in the extraction conditions on total protein extraction rate, the high molecular weight component of the acid precipitation rate. The results show that using the a multistage countercurrent solid-liquid extraction technology can be in the same protein yield under the conditions of water consumption savings of 16% extract liquid higher 7S and 11S concentration, acid precipitation stage 7S and 11S yield than The traditional process of acid precipitation stage yield increased by 3%. Achieve a multistage countercurrent solid-liquid extraction process scale-up, obtained SPI crude protein content and nitrogen solubility index were 94.9%, 99.4%, higher than the traditional extraction process to obtain SPI. 4 by using hydrophobic interaction chromatography for purification of trypsin inhibitor in the whey, and to characterize the purification results using HPLC and MALDI-TOF. The results show that the hydrophobic chromatography is the effective separation and purification of the trypsin inhibitor, and purified trypsin inhibitor to aglucone, epoxy activated agarose gel as a carrier system to take affinity media, in a standard Characterization of trypsin solution was used as the sample to the affinity chromatography media. The results show that, to obtain two kinds of affinity medium can achieve the purification of trypsin. In this study, the multi-stage countercurrent solid-liquid extraction technology used in SPI extract, and the effects of different extraction conditions on protein yield and release rate, separation and purification of trypsin inhibitor whey wastewater establishment section water and improve protein yield of the the SPI extraction process has important reference value.

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CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Protein ( PrP ) and the chemical processing industry > Chemical processing > Various proteins
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