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Scallop BAC clones colocalization and physical map contig validation studies related

Author: HuangChao
Tutor: XiangJianHai
School: Graduate School , Chinese Academy of Sciences ( Institute of Oceanography )
Course: Marine biology
Keywords: Scallop BAC contig Physical map Chlamys Genome Sequencing Chromosomal localization scallop Nucleoside diphosphate kinase Map-based cloning Gene mapping Genetic linkage map Genetic improvement FISH Fingerprint analysis Scallop Probe labeling Scallop aquaculture Gene Chromosome walking
CLC: S917.4
Type: Master's thesis
Year: 2011
Downloads: 69
Quote: 0
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Abstract


Zhikong scallop(Chlamys farreri) is one of most economicly important aquaculture species in China. Physical map is a crucial tool for genome sequencing, gene mapping, genetic improvement and selective breeding. We have developed a genome-wide, BAC-based physical map for the species recently. A total of 81,408 clones from two scallop BAC libraries were fingerprinted using ABI 3130xl Genetic Analyzer. After Data processing, A total of 3,072 contigs were assembled. Each contig contains an average of 41.2 clones, with an average size of 521 Kb and the combined total size of approximately 1.6 Gb for all contigs, coverage of 1.3×the genome.Using the technology of BAC-FISH, we localize the BAC clones containing NDPK gene in a pair of homologous chromosomes. The result of the location of the two clones will certificate the correction of the integration of the graph. NDPK gene is a widely distributed, conserved enzyme of importance in the flow of information of cells. The localization of NDPK gene in chromosome will provide theory support for research of structures and functions and the application of the gene. This research is the practice of localizing genes of low copies. The result will provide reference to lucubrating and distinguish chromosomes and integrate of the physical map and cytogenetics map of Zhikong scallop. Otherwise, we could validate the two clones containing NDPK gene whether localized on the same position of chromosome, identification of chromosomes and integrate graphs.Meanwhile, we could use the technology of BAC-FISH to certificate the correction of the Physical map.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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