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mRNA Expression Regularity and Localization of GTHR in Uterus of the Estrous Cycle in YiMeng Black Goat

Author: ShiZhongQiang
Tutor: WangShuYing
School: Shandong Agricultural University
Course: Basic Veterinary Science
Keywords: FSHR LHR Quantitative PCR Estrous cycle Uterus Yimeng black goat
CLC: S827
Type: Master's thesis
Year: 2011
Downloads: 9
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Abstract

Yimeng black goat local varieties in Shandong Province, is a dual-purpose type of velvet, leather, meat, known for its large, fleshy physique, cashmere yield, but its low reproductive rate, an average of 110% lambing, and therefore improve its breeding The rate has very important significance to the development of animal husbandry. Follicle-stimulating hormone (FSH) to stimulate ovarian follicle development and maturation and granulosa cells produce estrogen, and through the influence of Sertoli cell function and thus indirectly affect sperm formation. Luteinizing hormone (LH) mainly to stimulate the theca cell androgen production, ovarian granulosa cells synthesize estrogen substrates, as well as the start the animals of ovulation and maintain luteal progesterone stimulate Ledig cells produced in the testis produce androgens suitable androgen environment for the development of sperm. Gonadotropin in combination with these gonadal organ specificity of the target cell membrane receptors, causing receptor conformational change, regulate the activity of adenylate cyclase, the catalytic conversion of adenosine triphosphate (ATP) to cyclic monophosphate adenosine (cAMP), Further dependence of cAMP protein kinase is activated, the synthesis of the corresponding mRNA, produces its receptor protein. Goat FSHR and LHR extragonadal tissue expression studies reported rarely. In order to explore the the low fan goats uterus FSHR and LHR receptor distribution and its mRNA expression variation, this study extracted total RNA from Yimeng black goat uterus, using the same RT-QPCR method, respectively, at different stages in the estrous cycle Yimeng the black goats uterus paragraphs FSHR gene and LHR gene quantitative analysis at the mRNA level. Immunohistochemical method study Yimeng black goat estrous cycle stage FSHR and LHR protein location in the uterus different parts of the distribution. The main results are as follows: 1 FSHRmRNA and LHRmRNA are expressed in the uterus throughout the estrous cycle. Average overall expression levels of water in FSHRmRNA to higher than LHRmRNA expression; FSHRmRNA in the uterine horn metestrous highest expression level, getting as low as proestrus minimum; highest expression level of the uterine caruncle diestrus to proestrus minimum, four periods between The difference was not significant (P gt; 0.05); cervix highest expression level in estrus, diestrus was the lowest significant difference (P lt; 0.05), with other periods. LHRmRNA in the uterine horn, the uterine caruncle cervix in diestrus expression highest significant difference (P lt; 0.05) and the other three periods. Lower expression levels of two genes in the body of the uterus, and the law is not obvious. Proestrus and estrus uterine horn and uterine meat Fu inherent layer scattered immune positive reaction; endometrial epithelium, uterine glands of the glandular cells and vascular endothelium of the epithelial cells are not found positive staining; in the uterus and sub- cervical glandular epithelial cells and luminal epithelial cells are displayed as a negative reaction; weak at other stages of the estrous cycle FSHR positive. Uterine horns and uterine the caruncle endometrial cavity epithelial cells in diestrus, presented strong positive LHR reaction; were not found to have a positive reaction in the lamina propria and muscle layer, and serosa; uterine body and cervix of the same negative reaction; estrous cycle other period LHR weak positive reaction.

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