Dissertation > Excellent graduate degree dissertation topics show

Swine Serologic Cross-Reactivity with Pandemic (H1N1) 2009 Influenza Virus in Parts of China

Author: GaoLei
Tutor: LiuSiDang
School: Shandong Agricultural University
Course: Basic Veterinary Science
Keywords: Cross-immunity Pandemic (H1N1) 2009 influenza virus Current epidemic swine influenza virus (H1N1) ELISA
CLC: S855.3
Type: Master's thesis
Year: 2011
Downloads: 69
Quote: 0
Read: Download Dissertation

Abstract


The pandemic (H1N1) 2009 influenza virus is a new triple swine-origin reassortant that has caused globally human to human transmission and developed into the first influenza pandemic of the twenty-first century. This specific reassortant had never been found in swine when it was first detected in humans. However, after the outbreak of pandemic influenza H1N1 2009 in human, the pandemic (H1N1) 2009 virus was isolated from swine in more than ten countries during May–November 2009. Moreover, studies had confirmed that pandemic (H1N1) 2009 virus could infect pigs and caused swine to swine transmission. As domestic pigs have been described as a hypothetical‘mixing-vessel’, mediating by reassortment the emergence of new influenza viruses with avian or avian-like genes into the human population, and triggering a pandemic associated with antigenic shift. Meanwhile, to this day, there is still no evidence of isolation of the pandemic (H1N1) 2009 virus from swine in the mainland of China and the immunity of Chinese domestic pigs to pandemic (H1N1) 2009 virus is unknown. So it is of vital importance to investigate the cross-immunity level of swine in China to this new influenza virus.In order to investigate whether field swine serums in China have cross-reactive antibodies against pandemic (H1N1) 2009 influenza virus, first we respectively amplified the HA1 gene of A/California/04/2009H1N1 (2009 pandemic H1N1 virus)and A/Swine/Shandong/LY/2008(H1N1) (current epidemic SIV H1N1) by PCR, and cloned the PCR products into plasmid pET-30a(+).The recombinant plasmid pET-HA1(2009 pandemic H1N1) and pET-HA1(current epidemic SIV H1N1) were both expressed in in E. coli BL21 strain by the induction of IPTG and the recombinant His-HA1 protein was purified by an Ni-affinity chromatography. Then we respectively developed two enzyme-linked immunosorbent assay (ELISA) to detect antibodies against pandemic (H1N1) 2009 influenza virus and Chinese current epidemic swine influenza A virus (H1N1), using recombinant His-HA1 protein as antigen. Seven hundred and eighty-five field swine serums and one hundred and forty-three human serum obtained from patients diagnosed with pandemic (H1N1) 2009 were collected and detected by these two ELISAs.Then we made correlation analysis between two ELISA results by software Statistical Product and Service Solutions (SPSS), which indicated that there was a significant linear positive correlation between detecting results of SIV (H1N1) HA1-ELISA and that of 2009H1N1 HA1-ELISA. According to this relationship, it could be considered that swine serum positive to SIV (H1N1) had cross-reactivity with 2009H1N1 while human serum obtained from obtained from patients diagnosed with pandemic (H1N1) 2009 had cross-reactivity with SIV (H1N1). Based on this it could be suggested pigs in China had partial immunity to pandemic (H1N1) 2009 virus and in swine serum, the antibody level against pandemic (H1N1) 2009 had direct proportion with that against current epidemic swine influenza virus (H1N1).Combined with the results of sequence alignments, it can be suggested that A/California/04/2009 H1N1 and A/Swine/Shandong/1123/2008(H1N1) may had cross-reactive epitopes, which was the cause of cross-immunity.In this study, we carried out investigated the swine serologic cross-reactivity with pandemic (H1N1) 2009 influenza virus in China. These results enriched the content of pandemic (H1N1) 2009 influenza virus epidemiology in China, and indicated we must enhance the need for systematic surveillance of SIV in China.

Related Dissertations

  1. Study on Bispecific Monoclonal Antibody to Pesticide Imidacloprid and Parathion-Methyl,S482.2
  2. Establishment of ELISA for Detecting Cd-MT of Pteria Penguin and Preliminary Study of ELISA-kit,X835
  3. The Research on Enzyme-Linked Immunosorbent Assay for Pretilachlor and Acetochlor,S482.4
  4. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  5. Epidemiologic Studies on Duck Circovirus Infection in East China and Development of Monoclonal Antibody Against Duck Circovirus,S858.32
  6. Establishment of ELISA Based on Recombinant Protein NS1 of Swine Influenza Virus and Nuclear Localization of SIV NS and NP Proteins,S858.28
  7. The Dynamical Evaluation of Three Methods to Diagnose Mycoplasma Pneumoniae Infection in Children,R725.6
  8. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  9. Adiponectin gene signal peptide region prokaryotic expression and ELISA detection method,R392.1
  10. Japanese encephalitis virus monoclonal antibodies and preliminary application,R392
  11. The Research of Situation of BVDV Infection in Swinery and Preliminary Analyze the Cause of BVDV Infection,S858.28
  12. Experimental Study of Effects of Hizikia Fusiformis to Reduce Serum Lipids and Antioxidant in Hyperlipenmia Rats,R285.5
  13. Study on Rapid Immunoassay of Small Molecules Organic Pollutants in Urine Samples of Gastric Cancer Patients,R735.2
  14. Prokaryotic Expression and Renaturation of Streptavidin,Q78
  15. Development of Biological Methods for Detection of Anisakid Larvae in Seafood,TS254.7
  16. Experimental study of multiple myeloma thrombosis related factors,R733.3
  17. A Molecular Epidemiology Study on the Associations Between the MTHFR Polymorphisms and Genetic Susceptibility of Acute Lymphoblastic Leukemia,R733.7
  18. The Effect on Immune Function of Bal b/c Mouse by SGT,R285.5
  19. The Preparation of Anti-ovalbumin Monoclone Antibody and the Quantitative Determination of Ovalbumin and Gliadin in Foods by ELISA,R155.5
  20. Development and Application of ELISA Method Based on PCV Rep and Cap Proteins,S855.3
  21. Developmental Changes in Concentrations of Vitellin, Vitellogenin and Vitellogenin Gene Expression during the Ovarian Development of Swimming Crab, Portunus Trituberculatus,S917.4

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Veterinary Infectious Diseases > Virus disease
© 2012 www.DissertationTopic.Net  Mobile