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Effect of Different Rootstocks on Tolerance of Grafted Cucumber to Low Temperature, Weak Light and Salt Stress

Author: TianXueMei
Tutor: WeiZuo
School: Shandong Agricultural University
Course: Olericulture
Keywords: Cucumber Grafting Rootstocks Low temperature and low light NaCl stress Principal Component Analysis
CLC: S642.2
Type: Master's thesis
Year: 2011
Downloads: 87
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Abstract


Cucumber (Cucumis sativus L.) in northern China thermophilic fruit and vegetable cultivation area larger one winter chilling and low light is an important factor to limit its high productivity and efficiency. Meanwhile, with the increase in facilities for the duration of use, soil salinization has been increasing, affecting growth and development of cucumber, led to the decline in yield and quality. Overcome cold damage and even salt damage is one of the urgent need to address an important issue in the current solar greenhouse cucumber production. Select resistant to low temperature and light intensity, salt-tolerant rootstock grafting cultivation facilities cucumber resistance, and effective way to obtain high yield and efficiency. The trial on the basis of collected the 15 cucumber rootstocks varieties and resistance to low temperature and poor light and salt tolerance identification, choose patience different rootstocks cucumber, research grafted seedlings of chilling and low light (5 ℃, 100μmol · m -2 · s -1 ) and NaCl (100 mmol · L -1 ) Physiological Response to explore differences in the mechanism, not only rich in vegetable grafting resilience theory, at the same time provide the basis for cucumber production reasonable choice of rootstock, alleviating stress disorder, to improve the yield and quality. The main findings are as follows: using low temperature and low light stress treatment, research rootstock seedling growth and physiological changes, through the establishment of the value of the membership function, and principal component regression analysis: y = 1.976 x 1 2 (leaf area relative increase in the amount of the membership function value) 1.943 x 3 (leaf electrolyte leakage membership function value) 0.176, based on the size of the y values ??can judge different rootstocks resistant to chilling under low light capabilities. The cluster analysis showed that Yunnan black seed pumpkin the Tieli anvil chilling and low light ability of rootstocks resistant, Japanese Tosa, Black Tatari Neko pumpkin, Green research rootstock One is for the resistance to low-temperature low-light ability rootstock improvement of new Tosa One pumpkin, Tie-ka ぶ と ji ャ su Suites, Hui Taro, Industries イ ブ Hikaru resistance to low-temperature low-light ability to moderate the rootstock, Fire Phoenix, シ Industries Hikaru Ailuropoda, グ リ ツ プ resistance to low-temperature low-light ability weaker rootstocks, と ki wa Ailuropoda ヮ-Z ki ra め ki is resistant to low temperature weak light weak rootstocks. By the seedling stage NaCl stress treatment, research rootstock seedling growth and physiological changes, through the establishment of the value of the membership function, and principal component regression analysis, y = 1.853 x 1 (salt stress index membership function value) 2.116 x 2 (plant height relative increase in the amount of the membership function value) 1.907 x 3 (leaf area relative increase in the amount of the membership function value) 0.076, based on the size of the y values You can judge the salt tolerance of different rootstocks. The cluster analysis showed that Yunnan black seed pumpkin Tieli anvil the new Japanese Tosa, Black Tatari Neko pumpkin, green research rootstock One for salt tolerance rootstock; the Tie-ka ぶ と, improved the new Tosa One pumpkin for salt tolerance rootstocks; ジ ャ su Suites, Hui Taro Industries イ ブ Hikaru と ki wa Ailuropoda ヮ-Z, グ リ ツ プ, ki ra め ki is weaker salt tolerance rootstock, Fire Phoenix, シ Industries Hikaru Ailuropoda for salt tolerance weak material. 3 ki ra め ki Fire Phoenix, iron force anvil and Yunnan black seed pumpkin four kinds of resistance to low-temperature low-light ability of different rootstock grafted 'Xintai dense thorn' cucumber, self - rooted for the control and study the seedlings at 5 ℃, 100μmol · m -2 · s -1 Low Light Stress Growth and physiological responses, results showed that: grafting recruits and high relative increase in leaf area and dry weight was significantly higher than OWN ROOT; electrolyte leakage and malondialdehyde content was significantly lower than that of rooted, figleaf gourd seedlings grafted lowest, followed Tieli anvil, the Fire Phoenix ki the ra め ki grafted seedlings; proline content in the leaves of grafted net photosynthetic rate (Pn) and peroxidase (POD), superoxide dismutase (SOD), hydrogen peroxide enzyme (CAT), ascorbate peroxidase (APX) activity was significantly higher than that of rooted, Yunnan black seed Pumpkin grafted seedlings highest Tieli anvil centered the the Fire Phoenix, and ki ra め ki grafted seedlings lowest. Chilling under low light stress conditions, grafted cucumber seedlings and from root seedling leaves of Cu / Zn-SOD, Mn-SOD and CAT gene mRNA relative expression to match changes in the amount of the corresponding activity changes, grafted seedlings relative gene expression amount significantly higher than the self - rooted, the grafted seedlings highest figleaf gourd and iron force. The the APX gene mRNA relative expression amount of change and activity changes are not exactly the same, there are other factors involved in the regulation of APX in, but the the grafted seedlings APX gene mRNA relative expression level is always higher than the self - rooted. 5 Select シ Industries Hikaru Ailuropoda new Japanese Tosa, the Tieli anvil and figleaf gourd salt tolerance of different rootstocks 'Xintaimici' cucumber, OWN ROOT control to study 100 mmol · L - 1 NaCl stress on seedling growth and physiological metabolism results: NaCl stress, grafted recruits and high leaf area, dry weight by the degree of inhibition was significantly lower than that of rooted, electrolyte leakage and malondialdehyde The content is also significantly lower than the self - rooted in Yunnan black seed pumpkin grafted seedlings lowest, followed by is iron force anvil Japan new soil Zuo and シ エ Hikaru Ailuropoda grafted seedlings; leaves of grafted proline, soluble sugar content and POD, SOD, CAT, APX activity was significantly higher than the self - rooted figleaf gourd highest grafted seedlings, the Tieli anvil and the new Japanese Tosa grafted seedlings were no significant differences between. シ Industries ru パ the grafted seedlings lowest. NaCl stress after grafting seedling leaves Na content Yunnan black seed pumpkin lt; iron forces anvil lt; new Japanese Tosa lt; シ lt Industries Hikaru パ; OWN ROOT; K content in Yunnan black seed pumpkin, the difference between the the Tieli anvil and the new Japanese Tosa grafted seedlings not シ Industries Hikaru Ailuropoda grafted seedlings were significantly higher than the lowest content OWN ROOT; grafted seedlings of Na / K The ratio was significantly lower than that of rooted, the grafted seedlings lowest figleaf gourd. NaCl stress conditions, the grafted cucumber seedlings leaves Cu / Zn-SOD, Mn-SOD, CAT and APX gene mRNA relative expression were higher than the self - rooted to the the the figleaf gourd grafted seedlings highest, シ Industries ru パ the lowest. Grafting seedling leaves Mn-SOD, APX gene mRNA relative expression of the amount of the increase is an important reason to maintain the higher Mn-SOD and APX. The increase of NaCl stress, Yunnan black seed pumpkin, iron force anvil grafting seedling leaves of Cu / Zn-SOD and CAT activity of the change and its mRNA relative expression in keeping with the amount but シ Industries Hikaru Ailuropoda grafted seedlings and cucumber self-root seedlings Cu / Zn -SOD and CAT mRNA relative expression level showed a downward trend, is not fully consistent with the activity changes, there are other factors involved in the regulation of enzyme activity.

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