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The Interaction between Gene PDSS2 and Small G Protein RhoA in Regulating Gastric Cancer Proliferation

Author: WangZheng
Tutor: LiJianDing
School: Shanxi Medical
Course: Medical Imaging and Nuclear Medicine
Keywords: AGS Cell cycle GTPsea Prenyl diphosphate synthase subunit 2
CLC: R735.2
Type: Master's thesis
Year: 2011
Downloads: 65
Quote: 0
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Abstract


Objective: In china gastric cancer is an important disease with a very high fatality rate, and how to get effective early diagnosis and treatment is the main points of today’s study, the current medical imaging research focus on molecular imaging fields, including the targeted gene and protein markers and tracing and so on. PDSS2 recently been discovered as a new candidate tumor-related genes may be involved in a variety of gastric cancer development and progression of cancer. Its impact on the role of tumor rarely carried out, and the mechanism is not clear. Small G proteins are a class of small monomeric G protein, has been recognized widely involved in tumor cell proliferation, invasion and metastasis, apoptosis. The RhoA, Rac1, Cdc42, of Rho family as the representatives of small molecular protein well known. This object to choose gastric adenocarcinoma cell line AGS to study, observe PDSS2 gene’s function in AGS cell growth and relationship between RhoA, Rac1, Cdc42.This could give some important reference of gastric cancer study in molecular imaging .Methods:PDSS2 gene of cell line AGS was knockout by PDSS2 siRNA. Check the AGS cell cycle by flow cytometry after the PDSS2 siRNA transfection. Also Rho A, Rac1 and Cdc42, members of small G protein family, mRNA relative expression level was observed by Real Time-PCR after PDSS2 siRNA transfection.Result:After PDSS2 siRNA 24 hour-transfection, the efficiency is 98%. The level of both mRNA and protein are down expression after PDSS2 knockout. Cell Cycle analysis shows that AGS cell line proliferation is inhibited by arresting the cell cycle in G0/G1 phase,however, the cell event in G2/M phase in siRNA interference group is lower than that in control group. And real time PCR result reports that Rho mRNA expression level is down regulated by PDSS2 knockout.Conclusion:PDSS2 plays an important role in AGS cell proliferation by cell cycle regulation. And RhoA, one of small G protein members, is involved in this mechanism.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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