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Purpose: application of immunohistochemistry to detect cyclooxygenase -2 (Cyclooxygenase-2 and COX-2) in the normal cervix, cervical intraepithelial neoplasia (Cervical intraepithelial neoplasia, CIN) and cervical cancer tissue expression levels differences, to explore the development of the relationship between COX-2 and cervical cancer. 2. Detect COX-2 before radiotherapy, the expression changes after cervical squamous cell carcinoma, explore the relevance of COX-2 in cervical squamous cell carcinoma radiotherapy. Methods: Immunohistochemistry SP method detected 20 cases of normal cervical of COX-2 expression in cervical cancer tissue samples of 30 cases of CIN and 61 cases of direct surgery, simultaneous detection of COX-2 prior to radiotherapy, 29 cases of cervical squamous cell carcinoma expression and change. Application SPSS17.0 statistical software using x2 test and Mann-Whiteney test, P lt; 0.05 difference was statistically significant. Results: 1.COX-2 no expression in normal cervical tissue, in CIN tissue expression (positive expression rate of 56.67%), cervical carcinoma significantly increased (positive rate was 88.52%) among the three groups, the overall The difference was statistically significant (x2 = 39.01, P = 0.000 lt; 0.05). Pairwise comparisons among the three groups, the difference was statistically significant (P lt; 0.05). 2.COX-2 in adenocarcinoma positive expression rate was 91.67%, HSCORE median score of 135, with an average to 135.83 ± 54.85; COX-2 positive expression rate was 87.76% in squamous cell carcinoma, HSCORE score median 70, an average of 77.14 ± 49.16, and the difference was statistically significant (Z = -3.314, P = -0.001 lt; 0.05). COX-2 expression in cervical squamous cell carcinoma with lymph node metastasis, interstitial depth of invasion (P = 0.03 lt; 0.05; P = 0.006 lt; 0.05), and the differences were statistically significant; with clinical stage and lesions size, histological grade, no significant difference (P gt; 0.05). The 3.COX-2 expression in cervical squamous cell carcinoma before radiotherapy, after the organization, there are obvious changes, the difference was significant (Z = 3.192, P = 0.001 lt; 0.05). COX-2 in complete remission group (CR), there were statistically significant after cervical tissue expression before radiotherapy, after radiotherapy COX-2 was significantly decreased (Z = -2.647, P = 0.008 lt; 0.01); COX-2 in some before radiotherapy in remission (PR) after COX-2 expression showed a downward trend, but not statistically significant (Z = -1.652, P = 0.099 gt; 0.05). Between two different efficacy group after radiotherapy of COX-2 expression difference was statistically significant (Z = -2.923, P = 0.003 lt; 0.05), the expression of COX-2 in the complete remission group radiotherapy decreased more significantly. Conclusion: 1.COX-2 no expression in normal cervical tissue, increased expression in cervical intraepithelial neoplasia (CIN) organization, strong expression in cervical cancer, increase the expression of COX-2 enhanced with cervical lesions, suggesting that COX-2 may be involved process of development of cervical cancer. 2.COX-2 expression in adenocarcinoma was significantly higher than squamous cell carcinoma, COX-2 expression in cervical squamous cell carcinoma with lymph node metastasis, interstitial depth of invasion and clinical stage, tumor size, histological level between significant differences, suggesting that COX-2 may be involved in the invasion and metastasis of the cervix. The 3.COX-2 expression in cervical squamous cell carcinoma before radiotherapy, after the organization there are significant differences in COX-2 expression was significantly decreased overall trend after radiotherapy; complete remission (CR) after radiotherapy in decreased more significantly, in part remission group (PR) expression of a downward trend, but not statistically significant, suggesting that COX-2 expression and cervical cancer radiotherapy.
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