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Protective Effect of Combined Ganglioside GM1 and Shenfu Injection in Newborn Rats with Hypoxia-ischemic Brain Damage

Author: ZhangHaiJiao
Tutor: YinHuaiQing
School: Shanxi Medical
Course: Pediatrics
Keywords: Ganglioside Shenfu Injection Brain damage Apoptosis Bcl-2 Bax
CLC: R722.1
Type: Master's thesis
Year: 2011
Downloads: 42
Quote: 1
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Abstract


Objective: To neonatal hypoxic-ischemic brain injury (hypoxic-ischemic encephalopathy, HIE) refers to infants with perinatal asphyxia, resulting in brain tissue hypoxia injury, often the cause of early neonatal death, children with mental and somatic development obstacle to an important cause. HIE complex pathogenesis, apoptosis is an important cause of late nerve cell loss and dysfunction. Therefore, appropriate treatment measures taken according to the pathogenesis of the disease has a very important role in the prognosis of HIE. This experiment focuses on hypoxic-ischemic brain injury after application ganglioside Shenfu Injection their brain tissue of Bcl-2, Bax changes in trend analysis from a theoretical point of view, the joint application of two drugs on the nervous system function in neonatal rats HIE recovery is more obvious improvement. Methods: (1) Animal groups: newborn 7 days 90 SD rats were randomly divided into sham operation group, ischemia and hypoxia group (HIBD group), ischemia and hypoxia GM1 intervention group (GM1 group), ischemia and hypoxia Shenfu intervention group (Shenfu Group) and hypoxic-ischemic GM1 and Shenfu intervention group (combination group) five groups, 18 in each group. Depending on the time of the animals were killed after four groups made HIBD model divided into three subgroups: 6h, 24h, 48h group, six in each subgroup. (2) model preparation: sham group give the neck middle of incision and free the left common carotid artery, not hypoxia ischemia. Placed in a hypoxic environment 2h prepared from the model group after ligation of the left common carotid artery. The GM1 group and the SFI group were given intraperitoneal injection of GM1 immediately after hypoxic-ischemic or Shenfu Injection. Intraperitoneal injection of two drugs in the combination group on hypoxic-ischemic. (3) observe the changes in the brain tissue: the animals in each group were sacrificed at the corresponding time points, whichever is the brain tissue, visual observation of changes in the general form of the brain tissue, the HE staining light microscope to observe the pathological changes of the left side of the brain tissue, immunohistochemistry semi-quantitative detection of the left side of brain tissue Bcl-2, Bax expression. Results: (1) changes in behavior: neonatal rats after hypoxic-ischemic varying degrees of abnormal behavior. (2) naked eye observation: the brain tissue of sham group generally did not change significantly; brain tissue of HIBD group general form of varying degrees of abnormal changes; each treatment group compared with HIBD group changed significantly reduced. (3) HE staining results: the hippocampus of the sham group organizational structure is basically normal cells arranged in neat close morphologically normal. High magnification visible neurons nuclear large and round, transparent; the HIBD group of loose structure of the hippocampus, cerebral cortex, hippocampus cells arranged in disorder, and the vacuolar degeneration and necrosis, normal neuron cells decreased; intervention groups at each time point The degree of injury is significantly reduced compared to the HIBD group, the cerebral cortex, hippocampus cell degeneration and necrosis significantly reduced cell arrangement still rules, especially in the combination group changed significantly. (4) immunohistochemical staining results: (1) the expression of Bcl-2: Bcl-2 positive cells widely expressed in the cerebral cortex and hippocampus, cytoplasm coloring, dyeing brownish yellow or yellow-brown particles. HIBD and each treatment group, Bcl-positive expression peak in the 24 hours after HIBD. Sham group lowly, no significant difference at all time points; HIBD group at each time point Bcl-2 expression is increased, the average gray value of positive cells compared to the sham group, the differences were statistically significance (P lt; 0.01 ); positive expression of Bcl-2 GM1 group, SFI group and the combination group at each time point compared HIBD group further increased to further reduce the average gray value of positive cells compared HIBD group, the differences were statistically significant (P lt; 0.01); The combination group at each time point the expression of Bcl-2 positive than GM1 group and the Shenfu group to increase the degree of more obvious differences were statistically significant (P lt; 0.01). The ② Bax expression: Bax cells mainly expressed in the cytoplasm, stained brown particles. HIBD group and treatment group Bax positive peak expression at 24 hours after HIBD. A very small amount of Bax protein expression in the sham group; of HIBD group at all time points Bax protein expression increased significantly deepest stain positive average gray value expression compared with the sham group was significantly lower, the difference had statistical significance (P lt; 0.01 ); the Bax positive expression of GM1 group, SFI group and the combination group at each time point compared HIBD group decreased expression of positive average gray value than HIBD group increased, the differences were statistically significant (P lt; 0.01); combined group point-in-time expression of Bax positive than GM1 group and the Shenfu group decreased more obvious, the differences were statistically significant (P lt; 0.01). Conclusion: (1) the successful establishment of hypoxic-ischemic brain injury model after a single intraperitoneal injection of gangliosides or Shenfu injection can be varying degrees of to reduce neonatal rat brain damage, joint use of intraperitoneal injection of the two drugs their brain injury improving effect is more apparent. (2) ganglioside or Shenfu injection can be to play the role of the brain tissue upregulation of Bcl-2, Bax expression decreased to change the brain tissue Bcl-2 / Bax ratio, thereby reducing HIBD pathological damage and inhibition of neuronal apoptosis.

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CLC: > Medicine, health > Pediatrics > Newborns, premature children disease > Neonatal disease
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