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Objective: To observe the intermittent administration of recombinant human parathyroid hormone (1-34) [rhPTH (1-34)] in vitro osteoblast proliferation and type I collagen protein (Collagen I) and Osterix mRNA expression, explore rhPTH ( 1-34) on osteoblasts in vitro mechanism of action, provide some theoretical basis for the clinical use of drugs. Method: 1, cultured in vitro osteoblast and observe the morphology and function. Neonatal rat osteoblasts in vitro model will become bone cells were divided into a control group and 10 -11 10 -10 , 10 - 9 , 10 -8 , 10 -7 M rhPTH (1-34) 5 Ge dose administered group, a culture period of 24 hours 12 hours plus rhPTH (1-34), only the first 48 hours after using the MTT assay cell proliferation, measured by semi-quantitative RT-PCR method the osteoblasts Collagen I, Osterix mRNA expression. 3, the experimental data statistical methods expressed as mean ± standard deviation, using SPSS13.0 statistical software to analyze the experimental data, each group in general, there is no difference in using one-way ANOVA, pairwise comparison between groups using LSD analysis, P lt; 0.05 was considered statistically significant. Results: 1 cultured neonatal rat bone cells mostly fusiform, triangular or irregular polygons, rounded or oval nucleus, center or biased towards one side, surrounding cells to processes connected. Alkaline phosphatase staining was positive, cytoplasmic grayish black particles; alizarin red stained crimson nodules. 2, the different concentrations rhPTH (1-34) acting on osteoblasts, each group OD values ??were higher in 10 -1 1-10 -9 M was dose-dependent, 10 -9 M proliferation highest. Comparison to the control group and the experimental group, the experimental group group, the difference was statistically significant (P lt; 0.05). 3, different concentrations of rhPTH (1-34) acting on osteoblasts, Collagen I Osterix mRNA expression increased the ratio of increase in the optical density in the 10 -1 1-10 -9 M dose-dependent manner, 10 -9 M highest expression. Comparison to the control group and the experimental group, the experimental group group, the difference was statistically significant (P lt; 0.05). Conclusion: 1, recombinant human parathyroid hormone (1-34) can promote the proliferation of osteoblasts. 2, recombinant human parathyroid hormone (1-34) can increase Collagen I and Osterix mRNA expression. 3, recombinant human parathyroid hormone (1-34) may by regulation Collagen I and Osterix mRNA expression to promote osteoblast proliferation, differentiation and maturation.
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