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Experimental Study on the Apoptosis of Vascular Endothelial Cell Induced by the Intravenous Catheter System

Author: ChenHaiYan
Tutor: LuXueQin；YinMingWei
School: Henan University
Course: Nursing
Keywords: Intravenous catheter Retention time Vascular endothelial cells Apoptosis Detect
CLC: R472
Type: Master's thesis
Year: 2011
Downloads: 113
Quote: 0
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Abstract


The purpose of this project through animal experiments, intravenous catheter simulated clinical infusion detect impaired vascular endothelial cell apoptosis, to study the relationship between vascular endothelial cell apoptosis and vein damage, to explore the mechanisms of vascular injury caused by indwelling needle for clinical intravenous catheter applications and prevention of complications provide a theoretical basis. Methods rabbit ear vein as experimental vascular research two steps. Step 1: Create a phlebitis model 20 election in New Zealand white rabbits, right ear and the outer edge of the vein is not to take measures to control vessels. Left ear, the outer edge of the vein indwelling catheter catheter, as an experimental vessel, intravenous saline 20ml, 1 times / d after administration of heparin saline sealed tube. Indwelling 168 h extubation vivo after intraperitoneal anesthesia drawn Bouin's fixative 24h, dehydrated, embedded in paraffin, and serial sections (experimental vascular puncture point to the direction of the proximal end of the slice). The two groups of blood vessels are the statutory testing with HE staining and TUNEL semi-quantitative detection of vascular endothelial cell apoptosis. Step 2: grouping experiments selected 30 New Zealand white rabbits were randomly divided into 6 groups (n = 5), the double ear vein as experimental vascular indwelling catheter fixed intravenous saline 20ml, 1 / d after administration of heparin saline sealed tube. In medication 48h, 72h, 96h, 120h, 144h, 168h extubation after intraperitoneal anesthesia cut a live specimen. Drawn method: puncturing a blood vessel the midline width 0.1cm, the puncture site is marked distal end of 0.5cm, the proximal end of a total length of about 2.5cm 3.0cm of rectangular specimens, interception puncture point at both ends of 0.5cm the indwelling needle cannula at the end of the two sections of specimens of both ends of 0.5cm, were placed in Bouin's fixative fixed 24h. Conventional dehydrated, and embedded in paraffin. From the puncture site, intravenous indwelling the end of the needle cannula to the direction of the proximal end of each section specimens sliced ??4 for the qualitative detection of HE staining each piece of sections 1, 3, 2,4 Zhang slice for semi-quantitative detection of TUNEL method, observation vascular endothelial apoptosis circumstances. Results indwelling 168h lead to vascular injury compared with normal vascular endothelial cell apoptosis positive rate and the degree of apoptosis differences with statistical significance (** P lt; 0.01). 2 the same retention time, the the different the sliced ??parts endothelial apoptosis-positive rate and the degree of apoptosis. At the end of the needle cannula puncture site with intravenous indwelling vascular endothelial cell apoptosis positive rate and the degree of apoptosis difference was statistically significance (P lt; 0.05). 3. Different parts of the same slice, indwelling time endothelial cell apoptosis positive rate and the degree of apoptosis. With indwelling extension of time, the degree of vascular endothelial cell apoptosis gradually increased, the difference was statistically significance (** P lt; 0.01) apoptosis positive rate between the groups and the degree of apoptosis. Conclusion 1. Venous catheter mechanical stimulation can induce apoptosis in endothelial cells increased vascular injury. Vascular endothelial cell apoptosis intravenous catheter mechanical irritation related to the degree of venous indwelling needle aspiration at the point of vascular endothelial cell apoptosis positive rate and apoptosis severity than indwelling needle sleeve at the end. Degree of vascular endothelial cell apoptosis and the retention time, the retention time of 96h vascular endothelial cells, significant apoptosis phenomenon, optimal indwelling time should not exceed the 96h.

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