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The purpose of the use of color Doppler ultrasound detects rabbit renal ischemia-reperfusion injury after renal hemodynamics, analysis of tubular epithelial Bcl-2 expression, color Doppler ultrasound evaluation of renal ischemia-reperfusion the value of the degree of injury. METHODS: A rabbit renal ischemia-reperfusion model, 56 weight 1.8-2.4kg Japanese rabbits were randomly divided into three groups: normal control group (control group) 8, ischemia-reperfusion group (I / R group) 24 only the sham group (S) 24. Ischemia-reperfusion group after anesthesia, laparotomy, resection of the right kidney, the blood supply is restored after clipping the left renal artery 1h divided into different reperfusion time 2h, 8h, 24h subgroups. Sham group only anesthesia open belly isolated renal artery, blocking blood flow; 2h, 8h, 24h three subgroups divided according to the retention of the left kidney time. Rabbits were sacrificed animals in each group at a predetermined time points to detect renal artery hemodynamic changes (Vmax, Vd, Tamax, PI, RI), renal tissue. Paraffin-embedded sections, HE staining analysis of the degree of renal tissue injury, renal tubules Bcl-2 protein expression levels detected by immunohistochemistry (SABC). Image Plus Pro 6.0 image analysis software to analyze and calculate the mean optical density value of positive cells. Results ischemia-reperfusion renal hemodynamics: normal control group, sham-operated subgroups were compared, 2h subgroup reperfusion renal aortic the segmental arteries interlobar arteries Vmax, Vd, Tamax , PI, RI change had no significant difference (P gt; 0.05); sham-operated subgroups were compared with the normal control group, reperfusion 8h subgroups renal artery Vmax of the Vd, Tamax, PI, RI changes had no significant difference (P gt; 0.05), segmental arteries, interlobar artery RI increased (P lt; 0.05); sham-operated subgroups were compared with normal control group, reperfusion 24h subgroups renal artery, segmental arteries Vmax increased, interlobar arteries showed increased PI and RI (P lt; 0.05), while no significant differences (P gt the Vd and Tamax change; 0.05). 2. Renal pathological changes: sham group tubular no obvious lesions. Ischemia reperfusion 2h, 8h, 24h renal tubular epithelial cells with varying degrees of swelling, luminal expansion, vacuolization seen in some cells, the heaviest 24 tubular injury, we can see a large number of necrotic cells, interstitial inflammation cell infiltration. Renal tissue expression of Bcl-2: sham group tubular epithelial Bcl-2 was weakly positive expression, with the extension of time to retain the left kidney, divided into 2h, 8h, 24h three time points, the average optical density values ??were 0.2487 ± 0.0232,0.2516 ± 0.0347,0.2613 ± 0.0414, no significant difference in mean optical density value of the Bcl-2 expression between each time point; Bcl-2 expression increased after ischemia-reperfusion, expressed mainly in the outer medulla, and far The convoluted tubule significantly closer tubule strong With ischemia reperfusion time increased expression range. Ischemia reperfusion group was divided into 2h, 8h, 24h three time points, Bcl-2 expression mean optical density values ??were 0.2757 ± 0.0321,0.2833 ± 0.0214,0.3312 ± 0.0523 were higher than the corresponding group of sham group, and gradually increased with ischemia reperfusion time, reached the peak at 24h reperfusion increased Bcl-2 protein with ischemia-reperfusion injury aggravated expression. 4. Renal aorta, segmental arteries, the interlobar arteries Vmax PI, RI and the expression of Bcl-2 positive linear correlation which indicators at all levels of the artery RI values ??and interlobar arteries is the most obvious. Conclusions in rabbit renal ischemia-reperfusion experiments, color Doppler ultrasound monitoring of real-time quantitative rabbit ischemic reperfusion injury within 24 hours after the kidney hemodynamics; Bcl-2 expression increased after ischemia-reperfusion mainly expressed in the outer medulla and distal convoluted tubule, significantly closer convoluted tubule, ischemia-reperfusion prolonged expression range increase, reached a peak at 24h of reperfusion; renal artery hemodynamics and tubular Bcl-2 expression of significant positive linear correlation exists; Color Doppler ultrasound is expected to become a practical technology evaluation rabbit renal ischemia-reperfusion injury severity and prognosis.
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