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Under simulated physiological conditions, the purpose of research the two coumarins active ingredients imperatorin (imperatorin) and the different European decursin (isoimperatorin) and three chalcone ketones chalcone ketone (chalcone), 4 ' - methoxy-(4'-methoxychalcone) chalcone Chalcone, 4'-chloro-(4'-chlorochalcone) with biological macromolecules calf thymus DNA (ct-DNA), bovine serum albumin (BSA), human serum albumin (HSA), lysozyme (LYSO) interactions, and to explore the structure-activity relationship between them; interaction of the five compounds with HSA, fluorescence spectrometry to establish the quantitative determination of HSA in biological samples. Methods UV difference spectra method concept Chaou decursin and different before and after the the European decursin DNA by UV absorption spectra change; relevant parameters of the the two coumarins active ingredients with DNA using fluorescence spectroscopy and force type determine imperatorin salt effect on DNA thermal denaturation temperature, viscosity experiments investigated Mg 2 seven metal ions such as the impact on the the European decursin and isoimperatorin with DNA; prime and role model isoimperatorin with DNA. UV difference spectra method concept Chaou decursin to, isoimperatorin, chalcone, 4'-methoxy chalcone Chalcone, 4'-chloro serum albumin (SA) as well as three chalcone UV absorption spectra of ketones LYSO before and after; quenching mechanism using fluorescence spectrometry study five compounds with SA as well as the three chalcone ketones with LYSO interaction and related parameters; investigated Mg 2 < / sup> seven metal ions of the five compounds with SA interaction and a series of metal ions on the interaction of the three kinds of chalcone compounds with LYSO; synchronous fluorescence spectrometry study five compounds SA and three of chalcone compounds LYSO conformation. Using five compounds interact with HSA, using the established Quantitative determination of the fluorescence spectra of HSA content in the biological sample. Results for five compounds form 1:1 complexes with metal ions; the UV experimental results show that adding DNA Europe decursin, and isoimperatorin UV spectrum occurred hypochromic effect along is decursin and different imperatorin effect; assay results showed that the fluorescence intensity quenching of imperatorin and different European decursin DNA-berberine, tables along is decursin and isoimperatorin of berberine with DNA DNA binding competitive inhibition; the viscosity experiments show that imperatorin isoimperatorin make DNA viscosity first increases and then decreases. The UV results show that the five compounds with SA three chalcone ketones LYSO interact. Fluorescence spectroscopy showed that: five compounds SA, three chalcone compounds LYSO fluorescence quenching mechanisms are static quenching and non-radiative energy transfer force type are hydrogen bonds and van der Waals forces. Five compounds with BSA binding constants K were 1.48 × 10 4 , 1.04 × 10 4 , 3.16 × 10 5 , 3.02 × 10 5 and 2.94 × 10 5 L · mol -1 binding sites n for 0.915,0.742,0.996,0.999 and 0.991 respectively, combined distance r 0.73,0.66,3.35,3.22 and 3.39 nm; HSA binding constants K were 2.45 × 10 3 , 2.27 × 10 3 , 2.50 × 10 4 , 0.697 × 10 4 , 0.277 × 10 4 L · mol -1 , binding sites n respectively. The for 0.930,0.776,1.00,0.980 and 1.02, combined with the distance r were 2.97,2.74,3.93,3.78 and 4.25nm; the three chalcone ketones LYSO binding constants K were 2.90 × 10 < / sup>, 2.08 × 10 4 , 1.29 × 10 4 L · mol -1 , the number of binding sites n respectively 0.713,0.787 and 0.654, combined with the distance r respectively 4.23,4.45 and 4.25nm. Synchronous fluorescence results show that the the: five compounds SA conformational change, the three chalcone ketones LYSO the conformation change. Used to establish the measured fluorescence spectrometry in biological samples HSA, the average percentage of the mass fraction of 97.3%, 96.2%, 94.9%, 99.2% and 93.0%, the average recovery (98.5 ± 3.67)% and (99.5 ± 2.92 )%, (99.6 ± 2.14)%, (100 ± 1.4)% and (99.7 ± 2.03)%, the relative standard deviation of 3.7%, 2.9%, 2.2%, 1.4% and 2.0%, respectively. Conclusion imperatorin isoimperatorin with DNA binding, added on imperatorin and isoimperatorin and DNA binding of metal ions to produce varying degrees of impact. Salt effect, DNA melting experiments, experimental viscosity method table the along is decursin and mode of action of isoimperatorin with DNA intercalation with static mixing mode. Five compounds SA, three chalcone compound with LYSO interact. Produce different degrees of interaction between the metal ions on the five compounds with three globulin. Five compounds with HSA interaction, to establish the quantitative determination of biological samples HSA fluorescence spectrometry simple operation, high sensitivity, and provide a new method for the determination of the protein content.
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