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Objective: To observe the brain tissue around the hematoma after intracerebral hemorrhage in rats moisture content changes, neuronal apoptosis protease activated receptor -1 (PAR-1), hypoxia-inducible factor-1α (HIF-1α) expression and its vermiculite Long Huoxue Tongyu Capsule above expression, to further clarify the mechanisms of injury of cerebral hemorrhage and its possible cerebral protective effects for the clinical use of vermiculite dragon blood stasis capsules experimental theoretical basis for the treatment of cerebral hemorrhage. Methods: 1, the grouping method: 200 adult male SD rats were randomly divided into sham operation group, model group, vermiculite Lung promoting blood circulation stasis capsule, in the high-dose group (n = 40); 2, method of administration: vermiculite dragon promoting blood circulation and stasis capsule low, medium and high dose groups, respectively one week before modeling began promoting blood circulation and stasis capsule 0.4g/kg/d given vermiculite Long, 0.8g/kg/d 1.6g/kg/d gavage one week before the model group and the sham group in modeling given daily volume of saline; modeling method: application of rat brain stereotaxic apparatus directed, autologous blood injection in rat caudate nucleus preparation of a cerebral hemorrhage model; sham group volume of saline injection; 4, specimen preparation methods: respectively after 6h, 1d, 3d, 7d, each group randomly selected 10 rats, five brains were removed, with wet and dry weight measured brain tissue water content of 5 by cardiac perfusion fixation, the brain was sliced, the expression of apoptotic cells was determined by TUNEL PAR-1 protein and HIF-1α protein measured by immunohistochemistry method expressed. Results: 1, rats the general neurological signs score: Compared with the sham group, model group, and the vermiculite Long promoting blood circulation stasis capsule low, medium, and high dose group rats were significantly neurological signs score postoperative 6h, 1d, 3d increased significant difference (P lt; 0.01); compared with the model group, the high dose group rats neurological signs score after 6h lower, the difference was statistically significant (P lt; 0.05) in postoperative 1d, 3d were significantly reduced, there is a significant difference (P lt; 0.01), there are different degrees of reduction in the low-dose group were neurological signs score, but in addition to postoperative 1d (P lt; 0.01), the other point in time the difference was not statistically significant (P gt; 0.05); compared with the low-dose group, middle and high dose rats neurological signs score reduced in varying degrees, but the difference was not statistically significant (P gt; 0.05);, high dose rats neurological signs score group pairwise comparison, the difference was not statistically significant (P gt; 0.05). 2, brain water content, apoptotic cells, PAR-1, HIF-1α protein expression: the model group promoting blood circulation and vermiculite Long stasis capsule low, brain water content in the high-dose group, apoptotic cells, PAR-1, HIF -1α protein expression began to rise after 6h, 1d after further elevated, 3d peaked and began to decline, the 7th day after. Compared with the sham group, there is a significant difference (P lt; 0.01); compared with the model group, vermiculite dragon and blood stasis capsule, brain water content in the high-dose group, apoptotic cells, PAR-1 protein expression were decreased , HIF-1α protein expression increased, there is a significant difference (P lt; 0.01); compared with the low-dose group, brain water content, high dose group at each time point, apoptotic cells, PAR-1 protein expression decreased HIF-1α protein expression is elevated, there is a significant difference (P lt; 0.01);, high dose group comparison group, the difference was not statistically significance (P gt; 0.05). 3, correlation analysis: brain water content and the expression of apoptotic cells was positively correlated (r = 0.971, P = 0.000 lt; 0.05), PAR-1 expression was positively correlated (r = 0.988, P = 0.000 lt; 0.05), and HIF-1α expression was positively correlated (r = 0.901, P = 0.000 lt; 0.05). Conclusion: 1 vermiculite dragon blood stasis capsule can significantly improve the neurological deficit symptoms intracerebral hemorrhage in rats, its main mechanism by inhibiting PAR-1 activation, promote HIF-1α expression, inhibition of apoptosis, cerebral edema, in order to achieve the repair of damaged tissue. 2, vermiculite dragon promoting blood stasis capsule of intracerebral hemorrhage in rats has a significant role in brain protection, and there is a certain dose-effect relationship.
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